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Erythrocyte protoporphyrin/heme ratio in the assessment of iron status.
Clin Chem. 1979 Jan; 25(1):87-92.CC

Abstract

The protoporphyrinemia of iron deficiency is well recognized. Clinically, information on the protoporphyrin/heme molar ratio in whole blood offers certain advantages over protoporphyrin measurement alone. A procedure for determining this ratio is reported. Protoporphyrin is extracted, solubilized, and measured fluorometrically. Heme (as hemin chloride) is precipitated with the blood proteins, the precipitate is dissolved in an alkaline/pyridine solvent, and the resulting bispyridine ferriprotoporphyrin is measured spectrophotometrically. The molar ratio of these two metabolites correlates well with values for plasma ferritin, plasma iron, transferrin saturation, hemoglobin, and hematocrit. In some cases the ratio increases detectably while the other variables, especially hematocrit and hemoglobin, remain normal. Evidently it is a more sensitive index to iron status. For healthy men and women, the mean ratio is 16.0 (SD, 5.3). The mean + 3 SD, or a ratio of 32, is distinctly abnormal, as shown by a confirmatory test. We validated the test by surveying routine blood specimens obtained from several population groups.

Authors

Labbé RF
No affiliation info available
Finch CA
No affiliation info available
Smith NJ
No affiliation info available
Doan RN
No affiliation info available
Sood SK
No affiliation info available
Madan N
No affiliation info available

MeSH

Anemia, HypochromicErythrocytesHemeHumansIronPorphyrinsProtoporphyrinsSpectrophotometry

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

761386

Citation

Labbé, R F., et al. "Erythrocyte Protoporphyrin/heme Ratio in the Assessment of Iron Status." Clinical Chemistry, vol. 25, no. 1, 1979, pp. 87-92.
Labbé RF, Finch CA, Smith NJ, et al. Erythrocyte protoporphyrin/heme ratio in the assessment of iron status. Clin Chem. 1979;25(1):87-92.
Labbé, R. F., Finch, C. A., Smith, N. J., Doan, R. N., Sood, S. K., & Madan, N. (1979). Erythrocyte protoporphyrin/heme ratio in the assessment of iron status. Clinical Chemistry, 25(1), 87-92.
Labbé RF, et al. Erythrocyte Protoporphyrin/heme Ratio in the Assessment of Iron Status. Clin Chem. 1979;25(1):87-92. PubMed PMID: 761386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Erythrocyte protoporphyrin/heme ratio in the assessment of iron status. AU - Labbé,R F, AU - Finch,C A, AU - Smith,N J, AU - Doan,R N, AU - Sood,S K, AU - Madan,N, PY - 1979/1/1/pubmed PY - 1979/1/1/medline PY - 1979/1/1/entrez SP - 87 EP - 92 JF - Clinical chemistry JO - Clin Chem VL - 25 IS - 1 N2 - The protoporphyrinemia of iron deficiency is well recognized. Clinically, information on the protoporphyrin/heme molar ratio in whole blood offers certain advantages over protoporphyrin measurement alone. A procedure for determining this ratio is reported. Protoporphyrin is extracted, solubilized, and measured fluorometrically. Heme (as hemin chloride) is precipitated with the blood proteins, the precipitate is dissolved in an alkaline/pyridine solvent, and the resulting bispyridine ferriprotoporphyrin is measured spectrophotometrically. The molar ratio of these two metabolites correlates well with values for plasma ferritin, plasma iron, transferrin saturation, hemoglobin, and hematocrit. In some cases the ratio increases detectably while the other variables, especially hematocrit and hemoglobin, remain normal. Evidently it is a more sensitive index to iron status. For healthy men and women, the mean ratio is 16.0 (SD, 5.3). The mean + 3 SD, or a ratio of 32, is distinctly abnormal, as shown by a confirmatory test. We validated the test by surveying routine blood specimens obtained from several population groups. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/761386/Erythrocyte_protoporphyrin/heme_ratio_in_the_assessment_of_iron_status_ DB - PRIME DP - Unbound Medicine ER -