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Characterization of human homologue of 4-1BB and its ligand.
Immunol Lett. 1995 Feb; 45(1-2):67-73.IL

Abstract

The human homologue of 4-1BB (H4-1BB) cDNA was isolated from PMA plus ionomycin-treated human peripheral T-cell cDNA libraries. The amino acid sequence deduced from the nucleotide sequence showed that the protein is composed of 255 amino acids with 2 potential N-linked glycosylation sites. The molecular weight of its protein backbone is calculated to be 27 kDa. The H4-1BB contains features such as signal sequence and transmembrane domain, indicating that it is a receptor protein. This protein showed 60% identity of amino acid sequence to mouse 4-1BB. In the cytoplasmic domain there are 5 regions of amino acid sequences conserved from mouse to human, indicating that these residues might be important in the 4-1BB function. H4-1BB mRNA was detected in unstimulated peripheral blood T cells and was inducible in T-cell lines such as Jurkat and CEM. H4-1BB-AP, a fusion protein between the H4-1BB extracellular domain and alkaline phosphatase, was used to identify the ligand for the H4-1BB. Although the H4-1BB ligand was detected in both T and B cells of human peripheral blood, the ligand was preferentially expressed in primary B cells and B-cell lines. Daudi, a B-cell lymphoma, was one of the B-cell lines that carried a higher number of ligands. Scatchard analysis showed that the Kd = 1.4 x 10(9) M and the number of ligands in Daudi cell was 4.2 x 10(3).

Authors+Show Affiliations

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 56202-5120, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7622190

Citation

Zhou, Z, et al. "Characterization of Human Homologue of 4-1BB and Its Ligand." Immunology Letters, vol. 45, no. 1-2, 1995, pp. 67-73.
Zhou Z, Kim S, Hurtado J, et al. Characterization of human homologue of 4-1BB and its ligand. Immunol Lett. 1995;45(1-2):67-73.
Zhou, Z., Kim, S., Hurtado, J., Lee, Z. H., Kim, K. K., Pollok, K. E., & Kwon, B. S. (1995). Characterization of human homologue of 4-1BB and its ligand. Immunology Letters, 45(1-2), 67-73.
Zhou Z, et al. Characterization of Human Homologue of 4-1BB and Its Ligand. Immunol Lett. 1995;45(1-2):67-73. PubMed PMID: 7622190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of human homologue of 4-1BB and its ligand. AU - Zhou,Z, AU - Kim,S, AU - Hurtado,J, AU - Lee,Z H, AU - Kim,K K, AU - Pollok,K E, AU - Kwon,B S, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 67 EP - 73 JF - Immunology letters JO - Immunol Lett VL - 45 IS - 1-2 N2 - The human homologue of 4-1BB (H4-1BB) cDNA was isolated from PMA plus ionomycin-treated human peripheral T-cell cDNA libraries. The amino acid sequence deduced from the nucleotide sequence showed that the protein is composed of 255 amino acids with 2 potential N-linked glycosylation sites. The molecular weight of its protein backbone is calculated to be 27 kDa. The H4-1BB contains features such as signal sequence and transmembrane domain, indicating that it is a receptor protein. This protein showed 60% identity of amino acid sequence to mouse 4-1BB. In the cytoplasmic domain there are 5 regions of amino acid sequences conserved from mouse to human, indicating that these residues might be important in the 4-1BB function. H4-1BB mRNA was detected in unstimulated peripheral blood T cells and was inducible in T-cell lines such as Jurkat and CEM. H4-1BB-AP, a fusion protein between the H4-1BB extracellular domain and alkaline phosphatase, was used to identify the ligand for the H4-1BB. Although the H4-1BB ligand was detected in both T and B cells of human peripheral blood, the ligand was preferentially expressed in primary B cells and B-cell lines. Daudi, a B-cell lymphoma, was one of the B-cell lines that carried a higher number of ligands. Scatchard analysis showed that the Kd = 1.4 x 10(9) M and the number of ligands in Daudi cell was 4.2 x 10(3). SN - 0165-2478 UR - https://www.unboundmedicine.com/medline/citation/7622190/Characterization_of_human_homologue_of_4_1BB_and_its_ligand_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0165-2478(94)00227-I DB - PRIME DP - Unbound Medicine ER -