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Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus.
J Parasitol. 1995 Aug; 81(4):505-12.JP

Abstract

Cysteine protease activity was present in media collected after 24 hr in vitro culture of adult Haemonchus contortus. The released cysteine protease hydrolyzed the fluorogenic 7-amino-4-trifluoromethyl coumarin (AFC)-substituted synthetic peptides Z-phe-arg-AFC and Z-ala-arg-arg-AFC, but not Z-arg-arg-AFC or Z-arg-AFC, characterizing this activity as cathepsin L-like. Within the parasite, cysteine protease activity was highest in extracts of intestinal tissue. Secreted cysteine protease inhibited the clotting of sheep blood and hydrolyzed hemoglobin, fibrinogen, collagen, and IgG; the IgG hydrolysis site was within the hinge region. Four proteases with M(r) values of 30, 34, 37, and 41 kDa were identified with biotinylated-phenylalanine-arginine-fluoromethyl ketone, a specific probe that binds to active cysteine proteases. Adult parasites cultivated in the presence of 0.1 mM levamisole released 50% less protease activity compared to control cultures; in the presence of rafoxanide (0.1 mM), protease was not detected. Cathepsin L-like cysteine protease activity was released also by L4, but not the L3 larval stage. The active and developmentally regulated release of cysteine proteases by H. contortus may have a critical function in worm nutrition, immune evasion, or both.

Authors+Show Affiliations

Livestock and Poultry Sciences Institute, United States Department of Agriculture, Beltsville, Maryland 20705, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7623189

Citation

Rhoads, M L., and R H. Fetterer. "Developmentally Regulated Secretion of Cathepsin L-like Cysteine Proteases By Haemonchus Contortus." The Journal of Parasitology, vol. 81, no. 4, 1995, pp. 505-12.
Rhoads ML, Fetterer RH. Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus. J Parasitol. 1995;81(4):505-12.
Rhoads, M. L., & Fetterer, R. H. (1995). Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus. The Journal of Parasitology, 81(4), 505-12.
Rhoads ML, Fetterer RH. Developmentally Regulated Secretion of Cathepsin L-like Cysteine Proteases By Haemonchus Contortus. J Parasitol. 1995;81(4):505-12. PubMed PMID: 7623189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus. AU - Rhoads,M L, AU - Fetterer,R H, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 505 EP - 12 JF - The Journal of parasitology JO - J Parasitol VL - 81 IS - 4 N2 - Cysteine protease activity was present in media collected after 24 hr in vitro culture of adult Haemonchus contortus. The released cysteine protease hydrolyzed the fluorogenic 7-amino-4-trifluoromethyl coumarin (AFC)-substituted synthetic peptides Z-phe-arg-AFC and Z-ala-arg-arg-AFC, but not Z-arg-arg-AFC or Z-arg-AFC, characterizing this activity as cathepsin L-like. Within the parasite, cysteine protease activity was highest in extracts of intestinal tissue. Secreted cysteine protease inhibited the clotting of sheep blood and hydrolyzed hemoglobin, fibrinogen, collagen, and IgG; the IgG hydrolysis site was within the hinge region. Four proteases with M(r) values of 30, 34, 37, and 41 kDa were identified with biotinylated-phenylalanine-arginine-fluoromethyl ketone, a specific probe that binds to active cysteine proteases. Adult parasites cultivated in the presence of 0.1 mM levamisole released 50% less protease activity compared to control cultures; in the presence of rafoxanide (0.1 mM), protease was not detected. Cathepsin L-like cysteine protease activity was released also by L4, but not the L3 larval stage. The active and developmentally regulated release of cysteine proteases by H. contortus may have a critical function in worm nutrition, immune evasion, or both. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/7623189/Developmentally_regulated_secretion_of_cathepsin_L_like_cysteine_proteases_by_Haemonchus_contortus_ DB - PRIME DP - Unbound Medicine ER -