Tags

Type your tag names separated by a space and hit enter

Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins.
Proc Natl Acad Sci U S A. 1995 Jul 18; 92(15):6925-9.PN

Abstract

The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55 and rad57 mutant strains is suppressible by overexpression of RAD51 or RAD52. Virtually complete suppression is provided by the simultaneous overexpression of RAD51 and RAD52. This suppression occurs at 23 degrees C, where these mutants are more sensitive to x-rays, as well as at 30 degrees C and 36 degrees C. In addition, a recombination defect of rad55 and rad57 mutants is similarly suppressed. Direct in vivo interactions between the Rad51 and Rad55 proteins, and between Rad55 and Rad57, have also been identified by using the two-hybrid system. These results indicate that these four proteins constitute part of a complex, a "recombinosome," to effect the recombinational repair of double-strand breaks.

Authors+Show Affiliations

Department of Biochemistry, Stanford University School of Medicine, Stanford University, CA 94305, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7624345

Citation

Hays, S L., et al. "Complex Formation in Yeast Double-strand Break Repair: Participation of Rad51, Rad52, Rad55, and Rad57 Proteins." Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 15, 1995, pp. 6925-9.
Hays SL, Firmenich AA, Berg P. Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proc Natl Acad Sci U S A. 1995;92(15):6925-9.
Hays, S. L., Firmenich, A. A., & Berg, P. (1995). Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proceedings of the National Academy of Sciences of the United States of America, 92(15), 6925-9.
Hays SL, Firmenich AA, Berg P. Complex Formation in Yeast Double-strand Break Repair: Participation of Rad51, Rad52, Rad55, and Rad57 Proteins. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6925-9. PubMed PMID: 7624345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. AU - Hays,S L, AU - Firmenich,A A, AU - Berg,P, PY - 1995/7/18/pubmed PY - 1995/7/18/medline PY - 1995/7/18/entrez SP - 6925 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 92 IS - 15 N2 - The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55 and rad57 mutant strains is suppressible by overexpression of RAD51 or RAD52. Virtually complete suppression is provided by the simultaneous overexpression of RAD51 and RAD52. This suppression occurs at 23 degrees C, where these mutants are more sensitive to x-rays, as well as at 30 degrees C and 36 degrees C. In addition, a recombination defect of rad55 and rad57 mutants is similarly suppressed. Direct in vivo interactions between the Rad51 and Rad55 proteins, and between Rad55 and Rad57, have also been identified by using the two-hybrid system. These results indicate that these four proteins constitute part of a complex, a "recombinosome," to effect the recombinational repair of double-strand breaks. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/7624345/Complex_formation_in_yeast_double_strand_break_repair:_participation_of_Rad51_Rad52_Rad55_and_Rad57_proteins_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=7624345 DB - PRIME DP - Unbound Medicine ER -