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Laminin-rich extracellular matrix maintains high level of hepatocyte nuclear factor 4 in rat hepatocyte culture.
Biochem Biophys Res Commun. 1995 Jul 26; 212(3):800-5.BB

Abstract

Laminin-rich extracellular matrix, EHS-gel, has been demonstrated to keep a high level of liver-specific gene expression in cultured rat hepatocytes. To obtain information about the effect of EHS-gel on liver-specific functions, gene expression of liver-enriched transcription factors in rat hepatocytes was investigated. The apolipoprotein A-I and albumin mRNA levels were higher in hepatocytes cultured on EHS-gel than in those cultured on type I collagen (TIC). The levels of mRNA for HNF-4, C/EBP alpha and C/EBP beta were also higher on EHS-gel than on TIC. The level of HNF-4 mRNA in hepatocytes on EHS-gel was almost comparable to that in liver. The HNF-3 alpha mRNA level was lower on EHS-gel than on TIC. C/EBP beta mRNA was induced by dexamethasone in both EHS-gel and TIC. The induction of C/EBP alpha and HNF-4 by dexamethasone was observed only on TIC. These data suggest that EHS-gel leads hepatocytes to keep the phenotypic expression through high expression of liver-enriched transcription factors, such as HNF-4.

Authors+Show Affiliations

Department of Applied Biological Sciences, Nagoya University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7626113

Citation

Oda, H, et al. "Laminin-rich Extracellular Matrix Maintains High Level of Hepatocyte Nuclear Factor 4 in Rat Hepatocyte Culture." Biochemical and Biophysical Research Communications, vol. 212, no. 3, 1995, pp. 800-5.
Oda H, Nozawa K, Hitomi Y, et al. Laminin-rich extracellular matrix maintains high level of hepatocyte nuclear factor 4 in rat hepatocyte culture. Biochem Biophys Res Commun. 1995;212(3):800-5.
Oda, H., Nozawa, K., Hitomi, Y., & Kakinuma, A. (1995). Laminin-rich extracellular matrix maintains high level of hepatocyte nuclear factor 4 in rat hepatocyte culture. Biochemical and Biophysical Research Communications, 212(3), 800-5.
Oda H, et al. Laminin-rich Extracellular Matrix Maintains High Level of Hepatocyte Nuclear Factor 4 in Rat Hepatocyte Culture. Biochem Biophys Res Commun. 1995 Jul 26;212(3):800-5. PubMed PMID: 7626113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laminin-rich extracellular matrix maintains high level of hepatocyte nuclear factor 4 in rat hepatocyte culture. AU - Oda,H, AU - Nozawa,K, AU - Hitomi,Y, AU - Kakinuma,A, PY - 1995/7/26/pubmed PY - 1995/7/26/medline PY - 1995/7/26/entrez SP - 800 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 212 IS - 3 N2 - Laminin-rich extracellular matrix, EHS-gel, has been demonstrated to keep a high level of liver-specific gene expression in cultured rat hepatocytes. To obtain information about the effect of EHS-gel on liver-specific functions, gene expression of liver-enriched transcription factors in rat hepatocytes was investigated. The apolipoprotein A-I and albumin mRNA levels were higher in hepatocytes cultured on EHS-gel than in those cultured on type I collagen (TIC). The levels of mRNA for HNF-4, C/EBP alpha and C/EBP beta were also higher on EHS-gel than on TIC. The level of HNF-4 mRNA in hepatocytes on EHS-gel was almost comparable to that in liver. The HNF-3 alpha mRNA level was lower on EHS-gel than on TIC. C/EBP beta mRNA was induced by dexamethasone in both EHS-gel and TIC. The induction of C/EBP alpha and HNF-4 by dexamethasone was observed only on TIC. These data suggest that EHS-gel leads hepatocytes to keep the phenotypic expression through high expression of liver-enriched transcription factors, such as HNF-4. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/7626113/Laminin_rich_extracellular_matrix_maintains_high_level_of_hepatocyte_nuclear_factor_4_in_rat_hepatocyte_culture_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(85)72039-6 DB - PRIME DP - Unbound Medicine ER -