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Supplemented gamma-linolenic acid and eicosapentaenoic acid influence bone status in young male rats: effects on free urinary collagen crosslinks, total urinary hydroxyproline, and bone calcium content.

Abstract

The effect of different ratios of the prostaglandin precursors gamma-linolenic (GLA) and eicosapentaenoic (EPA) acids on bone status in growing rats measured as a function of free urinary pyridinium crosslinks and hydroxyproline levels was investigated. Male Sprague-Dawley rats were weaned onto an essential fatty acid deficient diet and from their fifth week, different groups of rats received a balanced, semisynthetic diet, supplemented with different ratios of GLA:EPA supplied as a mixture of evening primrose oil (EPO) and fish oil (FO). Controls were supplemented with linoleic (LA; sunflower oil) and alpha-linolenic (ALA; linseed oil) acids (3:1) or a commercially available rat chow. Animals were terminated at 84 days and femur length, ash weight, calcium content, free urinary pyridinium crosslinks (Pyd and Dpyd), total hydroxyproline (Hyp), and creatinine levels measured. Free urinary Pyd and Dpyd are good indicators of bone status and they correlated well with Hyp. Pyd and Dpyd excretion were significantly decreased in the higher GLA:EPA dietary groups and correlated well (r = 0.7) with Hyp levels. Concomitantly, bone calcium content increased significantly in the same dietary groups. These results suggest that diet supplementation with relatively high GLA:EPA ratios are more effective in inhibiting bone resorption than LA:ALA.

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  • Authors+Show Affiliations

    ,

    Department of Physiology, Faculty of Medicine, University of Pretoria, Republic of South Africa.

    , , , , ,

    Source

    Bone 16:4 Suppl 1995 Apr pg 385S-392S

    MeSH

    Amino Acids
    Animals
    Anti-Inflammatory Agents, Non-Steroidal
    Bone Resorption
    Calcium
    Eating
    Eicosapentaenoic Acid
    Fatty Acids
    Fatty Acids, Essential
    Femur
    Fish Oils
    Hydroxyproline
    Linoleic Acids
    Lipids
    Male
    Organ Size
    Plant Oils
    Rats
    Rats, Sprague-Dawley
    Spectrometry, Fluorescence
    gamma-Linolenic Acid

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    7626328

    Citation

    Claassen, N, et al. "Supplemented Gamma-linolenic Acid and Eicosapentaenoic Acid Influence Bone Status in Young Male Rats: Effects On Free Urinary Collagen Crosslinks, Total Urinary Hydroxyproline, and Bone Calcium Content." Bone, vol. 16, no. 4 Suppl, 1995, 385S-392S.
    Claassen N, Potgieter HC, Seppa M, et al. Supplemented gamma-linolenic acid and eicosapentaenoic acid influence bone status in young male rats: effects on free urinary collagen crosslinks, total urinary hydroxyproline, and bone calcium content. Bone. 1995;16(4 Suppl):385S-392S.
    Claassen, N., Potgieter, H. C., Seppa, M., Vermaak, W. J., Coetzer, H., Van Papendorp, D. H., & Kruger, M. C. (1995). Supplemented gamma-linolenic acid and eicosapentaenoic acid influence bone status in young male rats: effects on free urinary collagen crosslinks, total urinary hydroxyproline, and bone calcium content. Bone, 16(4 Suppl), 385S-392S.
    Claassen N, et al. Supplemented Gamma-linolenic Acid and Eicosapentaenoic Acid Influence Bone Status in Young Male Rats: Effects On Free Urinary Collagen Crosslinks, Total Urinary Hydroxyproline, and Bone Calcium Content. Bone. 1995;16(4 Suppl):385S-392S. PubMed PMID: 7626328.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Supplemented gamma-linolenic acid and eicosapentaenoic acid influence bone status in young male rats: effects on free urinary collagen crosslinks, total urinary hydroxyproline, and bone calcium content. AU - Claassen,N, AU - Potgieter,H C, AU - Seppa,M, AU - Vermaak,W J, AU - Coetzer,H, AU - Van Papendorp,D H, AU - Kruger,M C, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 385S EP - 392S JF - Bone JO - Bone VL - 16 IS - 4 Suppl N2 - The effect of different ratios of the prostaglandin precursors gamma-linolenic (GLA) and eicosapentaenoic (EPA) acids on bone status in growing rats measured as a function of free urinary pyridinium crosslinks and hydroxyproline levels was investigated. Male Sprague-Dawley rats were weaned onto an essential fatty acid deficient diet and from their fifth week, different groups of rats received a balanced, semisynthetic diet, supplemented with different ratios of GLA:EPA supplied as a mixture of evening primrose oil (EPO) and fish oil (FO). Controls were supplemented with linoleic (LA; sunflower oil) and alpha-linolenic (ALA; linseed oil) acids (3:1) or a commercially available rat chow. Animals were terminated at 84 days and femur length, ash weight, calcium content, free urinary pyridinium crosslinks (Pyd and Dpyd), total hydroxyproline (Hyp), and creatinine levels measured. Free urinary Pyd and Dpyd are good indicators of bone status and they correlated well with Hyp. Pyd and Dpyd excretion were significantly decreased in the higher GLA:EPA dietary groups and correlated well (r = 0.7) with Hyp levels. Concomitantly, bone calcium content increased significantly in the same dietary groups. These results suggest that diet supplementation with relatively high GLA:EPA ratios are more effective in inhibiting bone resorption than LA:ALA. SN - 8756-3282 UR - https://www.unboundmedicine.com/medline/citation/7626328/Supplemented_gamma_linolenic_acid_and_eicosapentaenoic_acid_influence_bone_status_in_young_male_rats:_effects_on_free_urinary_collagen_crosslinks_total_urinary_hydroxyproline_and_bone_calcium_content_ L2 - https://linkinghub.elsevier.com/retrieve/pii/875632829500062I DB - PRIME DP - Unbound Medicine ER -