Tags

Type your tag names separated by a space and hit enter

Insulin-like growth factor-II expression in carcinoma in colon cell lines: implications for autocrine actions.
J Am Coll Surg 1995; 181(2):145-54JA

Abstract

BACKGROUND

In the gastrointestinal tract, insulin-like growth factor-II (IGF-II) messenger RNA (mRNA) is localized mainly in mesenchymal cells, and is more abundant in the fetus than in the adult. The purposes of this study are to characterize the gene expression of IGF-II at the mRNA and protein level in seven different epithelial cell lines derived from colon carcinomas and to determine the action of IGF-II and IGF-receptors on a colon carcinoma cell line.

STUDY DESIGN

Insulin-like growth factor-II mRNAs were examined by Northern analysis; conditioned media from colon carcinoma cells were concentrated, chromatographed, and examined by a specific IGF-II radioreceptor assay. Insulin-like growth factor receptors were examined by radioligand binding assays. The mitogenic role of IGF-II was determined by a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay.

RESULTS

Multiple sizes of IGF-II mRNAs were expressed in all colon carcinoma cell lines tested (six human cell lines: HCT116, COLO 205, COLO 320 DM, LoVo, DLD-1, and HT29, and one mouse cell line: MC-26). In the conditioned media of COLO 205 and HCT116 cells, 7.5 kilodaltons IGF-II and high molecular form (IGF-II and IGF binding protein complex) were detected. Both Type I and Type II IGF receptors were present on COLO 205 cells whose growth was stimulated by IGF-II. Addition of anti-IGF-I receptor and anti-IGF-II antibody in the cell culture significantly depressed growth of the COLO 205 cell line in the presence or absence of exogenous IGF-II.

CONCLUSIONS

Insulin-like growth factor-II mRNAs are expressed in human and mouse colon carcinoma cell lines, which may induce production of a significant amount of biologically active IGF-II protein. The IGF-II secreted by COLO 205 cells may stimulate cell growth in an autocrine fashion through the Type I IGF receptors.

Authors+Show Affiliations

Department of Surgery, University of Texas Medical Branch, Galveston 77555-0527, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7627387

Citation

Guo, Y S., et al. "Insulin-like Growth factor-II Expression in Carcinoma in Colon Cell Lines: Implications for Autocrine Actions." Journal of the American College of Surgeons, vol. 181, no. 2, 1995, pp. 145-54.
Guo YS, Jin GF, Townsend CM, et al. Insulin-like growth factor-II expression in carcinoma in colon cell lines: implications for autocrine actions. J Am Coll Surg. 1995;181(2):145-54.
Guo, Y. S., Jin, G. F., Townsend, C. M., Zhang, T., Sheng, H. M., Beauchamp, R. D., & Thompson, J. C. (1995). Insulin-like growth factor-II expression in carcinoma in colon cell lines: implications for autocrine actions. Journal of the American College of Surgeons, 181(2), pp. 145-54.
Guo YS, et al. Insulin-like Growth factor-II Expression in Carcinoma in Colon Cell Lines: Implications for Autocrine Actions. J Am Coll Surg. 1995;181(2):145-54. PubMed PMID: 7627387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin-like growth factor-II expression in carcinoma in colon cell lines: implications for autocrine actions. AU - Guo,Y S, AU - Jin,G F, AU - Townsend,C M,Jr AU - Zhang,T, AU - Sheng,H M, AU - Beauchamp,R D, AU - Thompson,J C, PY - 1995/8/1/pubmed PY - 2001/3/28/medline PY - 1995/8/1/entrez SP - 145 EP - 54 JF - Journal of the American College of Surgeons JO - J. Am. Coll. Surg. VL - 181 IS - 2 N2 - BACKGROUND: In the gastrointestinal tract, insulin-like growth factor-II (IGF-II) messenger RNA (mRNA) is localized mainly in mesenchymal cells, and is more abundant in the fetus than in the adult. The purposes of this study are to characterize the gene expression of IGF-II at the mRNA and protein level in seven different epithelial cell lines derived from colon carcinomas and to determine the action of IGF-II and IGF-receptors on a colon carcinoma cell line. STUDY DESIGN: Insulin-like growth factor-II mRNAs were examined by Northern analysis; conditioned media from colon carcinoma cells were concentrated, chromatographed, and examined by a specific IGF-II radioreceptor assay. Insulin-like growth factor receptors were examined by radioligand binding assays. The mitogenic role of IGF-II was determined by a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay. RESULTS: Multiple sizes of IGF-II mRNAs were expressed in all colon carcinoma cell lines tested (six human cell lines: HCT116, COLO 205, COLO 320 DM, LoVo, DLD-1, and HT29, and one mouse cell line: MC-26). In the conditioned media of COLO 205 and HCT116 cells, 7.5 kilodaltons IGF-II and high molecular form (IGF-II and IGF binding protein complex) were detected. Both Type I and Type II IGF receptors were present on COLO 205 cells whose growth was stimulated by IGF-II. Addition of anti-IGF-I receptor and anti-IGF-II antibody in the cell culture significantly depressed growth of the COLO 205 cell line in the presence or absence of exogenous IGF-II. CONCLUSIONS: Insulin-like growth factor-II mRNAs are expressed in human and mouse colon carcinoma cell lines, which may induce production of a significant amount of biologically active IGF-II protein. The IGF-II secreted by COLO 205 cells may stimulate cell growth in an autocrine fashion through the Type I IGF receptors. SN - 1072-7515 UR - https://www.unboundmedicine.com/medline/citation/7627387/Insulin_like_growth_factor_II_expression_in_carcinoma_in_colon_cell_lines:_implications_for_autocrine_actions_ L2 - http://www.diseaseinfosearch.org/result/2708 DB - PRIME DP - Unbound Medicine ER -