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Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma.
J Clin Endocrinol Metab. 1995 Aug; 80(8):2342-6.JC

Abstract

The purpose of this study was to characterize the structure of big big PRL (bb-PRL) in patients with macroprolactinemia or prolactinomas. Serum samples from these patients were fractionated by Sephadex G-100 chromatography, and PRL was measured in the eluate by an immunoradiometric assay. The fractions containing bb-PRL were subjected to affinity chromatography with an antihuman immunoglobulin G (IgG) agarose column. PRL and IgG were assayed in the fractions obtained after affinity chromatography by immunoradiometric assay and radial immunodiffusion, respectively. bb-PRL was also immunoprecipitated with an antihuman PRL antibody, followed by polyacrylamide gel electrophoresis and Western blotting. We found that an average of 60% (range, 27-87%) of bb-PRL from patients with macroprolactinemia was retained by the agarose, indicating that this form of PRL contains an IgG. In one of the patients with prolactinoma, the big big form constituted 76% of the total PRL immunoreactivity. Most (75%) of the bb-PRL from this patient behaved as an IgG after affinity chromatography. In the two other patients with prolactinoma, we found that 22% and 25% of the bb-PRL, which represented only 2% and 3% of the total PRL in the serum, reacted as an IgG. In both groups of patients, the 23-kilodalton form of PRL was detected after the immunoprecipitation of bb-PRL. These results show that bb-PRL is in part a complex of 23-kilodalton PRL with an IgG and not an antibody mimicking the actions of PRL, as has been demonstrated for some large forms of growth-hormone.

Authors+Show Affiliations

Department of Endocrinology and Molecular Biology Laboratory, Portuguese Institute of Oncology, Lisbon.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7629227

Citation

Cavaco, B, et al. "Some Forms of Big Big Prolactin Behave as a Complex of Monomeric Prolactin With an Immunoglobulin G in Patients With Macroprolactinemia or Prolactinoma." The Journal of Clinical Endocrinology and Metabolism, vol. 80, no. 8, 1995, pp. 2342-6.
Cavaco B, Leite V, Santos MA, et al. Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. J Clin Endocrinol Metab. 1995;80(8):2342-6.
Cavaco, B., Leite, V., Santos, M. A., Arranhado, E., & Sobrinho, L. G. (1995). Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. The Journal of Clinical Endocrinology and Metabolism, 80(8), 2342-6.
Cavaco B, et al. Some Forms of Big Big Prolactin Behave as a Complex of Monomeric Prolactin With an Immunoglobulin G in Patients With Macroprolactinemia or Prolactinoma. J Clin Endocrinol Metab. 1995;80(8):2342-6. PubMed PMID: 7629227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. AU - Cavaco,B, AU - Leite,V, AU - Santos,M A, AU - Arranhado,E, AU - Sobrinho,L G, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 2342 EP - 6 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 80 IS - 8 N2 - The purpose of this study was to characterize the structure of big big PRL (bb-PRL) in patients with macroprolactinemia or prolactinomas. Serum samples from these patients were fractionated by Sephadex G-100 chromatography, and PRL was measured in the eluate by an immunoradiometric assay. The fractions containing bb-PRL were subjected to affinity chromatography with an antihuman immunoglobulin G (IgG) agarose column. PRL and IgG were assayed in the fractions obtained after affinity chromatography by immunoradiometric assay and radial immunodiffusion, respectively. bb-PRL was also immunoprecipitated with an antihuman PRL antibody, followed by polyacrylamide gel electrophoresis and Western blotting. We found that an average of 60% (range, 27-87%) of bb-PRL from patients with macroprolactinemia was retained by the agarose, indicating that this form of PRL contains an IgG. In one of the patients with prolactinoma, the big big form constituted 76% of the total PRL immunoreactivity. Most (75%) of the bb-PRL from this patient behaved as an IgG after affinity chromatography. In the two other patients with prolactinoma, we found that 22% and 25% of the bb-PRL, which represented only 2% and 3% of the total PRL in the serum, reacted as an IgG. In both groups of patients, the 23-kilodalton form of PRL was detected after the immunoprecipitation of bb-PRL. These results show that bb-PRL is in part a complex of 23-kilodalton PRL with an IgG and not an antibody mimicking the actions of PRL, as has been demonstrated for some large forms of growth-hormone. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/7629227/Some_forms_of_big_big_prolactin_behave_as_a_complex_of_monomeric_prolactin_with_an_immunoglobulin_G_in_patients_with_macroprolactinemia_or_prolactinoma_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.80.8.7629227 DB - PRIME DP - Unbound Medicine ER -