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Use of a stable copper isotope (65Cu) in the differential diagnosis of Wilson's disease.
Clin Sci (Lond). 1995 Jun; 88(6):727-32.CS

Abstract

1. 65Cu/63Cu stable-isotope ratios have been measured in blood serum after oral administration of the stable isotope 65Cu. The incorporation of the isotope into the plasma protein pool was followed at various times for up to 3 days. The resulting patterns of enrichment in healthy control subjects, in Wilson's disease patients and in heterozygotes for the Wilson's disease gene, were similar in appearance to those found by others using copper radioactive isotopes. After an initially high enrichment at 2 h after dosage, the Wilson's disease cases, in contrast to the control subjects, did not show a secondary rise in isotope enrichment of the plasma pool after 72 h, demonstrating a failure to incorporate copper into caeruloplasmin. The Wilson's disease heterozygotes had variable degrees of impairment of isotope incorporation, not always distinguished from those of control subjects. 2. The stability of the isotope also permits the copper tracer to be followed for a longer period. Ten healthy subjects were studied for over 40 days, allowing the biological half-time of an oral dose of copper to be determined (median 18.5 days, 95% confidence interval 14-26 days). Known heterozygotes for the Wilson's disease gene were found to have a significantly increased biological half-time for removal of copper from the plasma pool (median 43 days, 95% confidence interval 32-77 days). 3. The incorporation of 65 Cu in patients with diseases of the liver (other than Wilson's disease) was found to be similar to that in control subjects, aiding differential diagnosis.

Authors+Show Affiliations

Institute of Biochemistry, Royal Infirmary, Glasgow, U.K.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7634759

Citation

Lyon, T D., et al. "Use of a Stable Copper Isotope (65Cu) in the Differential Diagnosis of Wilson's Disease." Clinical Science (London, England : 1979), vol. 88, no. 6, 1995, pp. 727-32.
Lyon TD, Fell GS, Gaffney D, et al. Use of a stable copper isotope (65Cu) in the differential diagnosis of Wilson's disease. Clin Sci (Lond). 1995;88(6):727-32.
Lyon, T. D., Fell, G. S., Gaffney, D., McGaw, B. A., Russell, R. I., Park, R. H., Beattie, A. D., Curry, G., Crofton, R. J., & Gunn, I. (1995). Use of a stable copper isotope (65Cu) in the differential diagnosis of Wilson's disease. Clinical Science (London, England : 1979), 88(6), 727-32.
Lyon TD, et al. Use of a Stable Copper Isotope (65Cu) in the Differential Diagnosis of Wilson's Disease. Clin Sci (Lond). 1995;88(6):727-32. PubMed PMID: 7634759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of a stable copper isotope (65Cu) in the differential diagnosis of Wilson's disease. A1 - Lyon,T D, AU - Fell,G S, AU - Gaffney,D, AU - McGaw,B A, AU - Russell,R I, AU - Park,R H, AU - Beattie,A D, AU - Curry,G, AU - Crofton,R J, AU - Gunn,I, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 727 EP - 32 JF - Clinical science (London, England : 1979) JO - Clin Sci (Lond) VL - 88 IS - 6 N2 - 1. 65Cu/63Cu stable-isotope ratios have been measured in blood serum after oral administration of the stable isotope 65Cu. The incorporation of the isotope into the plasma protein pool was followed at various times for up to 3 days. The resulting patterns of enrichment in healthy control subjects, in Wilson's disease patients and in heterozygotes for the Wilson's disease gene, were similar in appearance to those found by others using copper radioactive isotopes. After an initially high enrichment at 2 h after dosage, the Wilson's disease cases, in contrast to the control subjects, did not show a secondary rise in isotope enrichment of the plasma pool after 72 h, demonstrating a failure to incorporate copper into caeruloplasmin. The Wilson's disease heterozygotes had variable degrees of impairment of isotope incorporation, not always distinguished from those of control subjects. 2. The stability of the isotope also permits the copper tracer to be followed for a longer period. Ten healthy subjects were studied for over 40 days, allowing the biological half-time of an oral dose of copper to be determined (median 18.5 days, 95% confidence interval 14-26 days). Known heterozygotes for the Wilson's disease gene were found to have a significantly increased biological half-time for removal of copper from the plasma pool (median 43 days, 95% confidence interval 32-77 days). 3. The incorporation of 65 Cu in patients with diseases of the liver (other than Wilson's disease) was found to be similar to that in control subjects, aiding differential diagnosis. SN - 0143-5221 UR - https://www.unboundmedicine.com/medline/citation/7634759/Use_of_a_stable_copper_isotope__65Cu__in_the_differential_diagnosis_of_Wilson's_disease_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/cs0880727 DB - PRIME DP - Unbound Medicine ER -