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Analysis of V beta 8.2 CDR3 sequences from spinal cord T cells of Lewis rats vaccinated or treated with TCR V beta 8.2-39-59 peptide.
J Immunol. 1995 Aug 01; 155(3):1556-64.JI

Abstract

Experimental autoimmune encephalomyelitis (EAE) in the Lewis rat can be induced with the administration of Gp-BP. This disease appears to be mediated at least in part by V beta 8.2+CD4+T cells, which specifically recognize the BP72-89 encephalitogenic peptide. Treatment or protection with V beta 8.2 CDR2 39-59 peptide can suppress or prevent clinical signs of EAE, presumably through the activation of regulatory T cells. Interestingly, V beta 8.2+ T cells continue to persist in the spinal cord of protected animals, although their appearance in the central nervous system (CNS) is delayed when compared with control animals with EAE. As part of our effort to elucidate the mechanism(s) of peptide protection and therapy, we sought to determine whether the V beta 8.2+ T cells in the spinal cord of protected or treated rats were truly representative of those found in rats with clinical EAE. Therefore, we examined the following CNS samples for the Asp96Ser97 motif, which has been identified previously in V beta 8.2+ BP-specific, encephalitogenic T cell clones: 1) rats protected with V beta 8.2-39-59 peptide, 2) rats treated with V beta 8.2-39-59 peptide, and 3) control rats with EAE. Our findings indicate that EAE-associated V beta 8.2+ sequences can still be found in both peptide-treated and peptide-protected rats. It appears that administration of V beta 8.2 CDR2 peptide does not prevent EAE-associated V beta 8.2+ T cells from infiltrating the CNS and that other mechanisms are at work to prevent the development of EAE.

Authors+Show Affiliations

Veterans Affairs Medical Center, Portland, OR 97201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7636217

Citation

Buenafe, A C., et al. "Analysis of V Beta 8.2 CDR3 Sequences From Spinal Cord T Cells of Lewis Rats Vaccinated or Treated With TCR V Beta 8.2-39-59 Peptide." Journal of Immunology (Baltimore, Md. : 1950), vol. 155, no. 3, 1995, pp. 1556-64.
Buenafe AC, Vainiene M, Celnik B, et al. Analysis of V beta 8.2 CDR3 sequences from spinal cord T cells of Lewis rats vaccinated or treated with TCR V beta 8.2-39-59 peptide. J Immunol. 1995;155(3):1556-64.
Buenafe, A. C., Vainiene, M., Celnik, B., Vandenbark, A. A., & Offner, H. (1995). Analysis of V beta 8.2 CDR3 sequences from spinal cord T cells of Lewis rats vaccinated or treated with TCR V beta 8.2-39-59 peptide. Journal of Immunology (Baltimore, Md. : 1950), 155(3), 1556-64.
Buenafe AC, et al. Analysis of V Beta 8.2 CDR3 Sequences From Spinal Cord T Cells of Lewis Rats Vaccinated or Treated With TCR V Beta 8.2-39-59 Peptide. J Immunol. 1995 Aug 1;155(3):1556-64. PubMed PMID: 7636217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of V beta 8.2 CDR3 sequences from spinal cord T cells of Lewis rats vaccinated or treated with TCR V beta 8.2-39-59 peptide. AU - Buenafe,A C, AU - Vainiene,M, AU - Celnik,B, AU - Vandenbark,A A, AU - Offner,H, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 1556 EP - 64 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 155 IS - 3 N2 - Experimental autoimmune encephalomyelitis (EAE) in the Lewis rat can be induced with the administration of Gp-BP. This disease appears to be mediated at least in part by V beta 8.2+CD4+T cells, which specifically recognize the BP72-89 encephalitogenic peptide. Treatment or protection with V beta 8.2 CDR2 39-59 peptide can suppress or prevent clinical signs of EAE, presumably through the activation of regulatory T cells. Interestingly, V beta 8.2+ T cells continue to persist in the spinal cord of protected animals, although their appearance in the central nervous system (CNS) is delayed when compared with control animals with EAE. As part of our effort to elucidate the mechanism(s) of peptide protection and therapy, we sought to determine whether the V beta 8.2+ T cells in the spinal cord of protected or treated rats were truly representative of those found in rats with clinical EAE. Therefore, we examined the following CNS samples for the Asp96Ser97 motif, which has been identified previously in V beta 8.2+ BP-specific, encephalitogenic T cell clones: 1) rats protected with V beta 8.2-39-59 peptide, 2) rats treated with V beta 8.2-39-59 peptide, and 3) control rats with EAE. Our findings indicate that EAE-associated V beta 8.2+ sequences can still be found in both peptide-treated and peptide-protected rats. It appears that administration of V beta 8.2 CDR2 peptide does not prevent EAE-associated V beta 8.2+ T cells from infiltrating the CNS and that other mechanisms are at work to prevent the development of EAE. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/7636217/Analysis_of_V_beta_8_2_CDR3_sequences_from_spinal_cord_T_cells_of_Lewis_rats_vaccinated_or_treated_with_TCR_V_beta_8_2_39_59_peptide_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=7636217 DB - PRIME DP - Unbound Medicine ER -