Tags

Type your tag names separated by a space and hit enter

Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression.
EMBO J. 1995 Aug 01; 14(15):3706-11.EJ

Abstract

The growth transforming potential of Epstein-Barr virus (EBV) for Burkitt's lymphoma and nasopharyngeal carcinoma is now extended to other neoplasia, such as Hodgkin's disease, peripheral T-cell tumor and gastric cancer. We have generated an EBV recombinant with a selectable marker at the viral thymidine kinase locus. Recombinant EBV was successfully infected into a human T-cell line, MT-2. Following incubation in the selective medium, drug resistant MT-2 cell clones were isolated and proved to be infected with recombinant EBV. EBV-infected MT-2 cell clones expressed EBNA 1 and LMP 1 and very little of EBNA 2, showing the BamHI F promoter-driven latency II form of infection, which is seen in non-B-cell tumors. This is the first report of in vitro generation of latency II type EBV infection. The present system of persistent EBV infection in T cells should be a good model for investigating the pathogenic role of EBV in non-B-cell tumors.

Authors+Show Affiliations

Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7641689

Citation

Yoshiyama, H, et al. "Persistent Epstein-Barr Virus Infection in a Human T-cell Line: Unique Program of Latent Virus Expression." The EMBO Journal, vol. 14, no. 15, 1995, pp. 3706-11.
Yoshiyama H, Shimizu N, Takada K. Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression. EMBO J. 1995;14(15):3706-11.
Yoshiyama, H., Shimizu, N., & Takada, K. (1995). Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression. The EMBO Journal, 14(15), 3706-11.
Yoshiyama H, Shimizu N, Takada K. Persistent Epstein-Barr Virus Infection in a Human T-cell Line: Unique Program of Latent Virus Expression. EMBO J. 1995 Aug 1;14(15):3706-11. PubMed PMID: 7641689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression. AU - Yoshiyama,H, AU - Shimizu,N, AU - Takada,K, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 3706 EP - 11 JF - The EMBO journal JO - EMBO J VL - 14 IS - 15 N2 - The growth transforming potential of Epstein-Barr virus (EBV) for Burkitt's lymphoma and nasopharyngeal carcinoma is now extended to other neoplasia, such as Hodgkin's disease, peripheral T-cell tumor and gastric cancer. We have generated an EBV recombinant with a selectable marker at the viral thymidine kinase locus. Recombinant EBV was successfully infected into a human T-cell line, MT-2. Following incubation in the selective medium, drug resistant MT-2 cell clones were isolated and proved to be infected with recombinant EBV. EBV-infected MT-2 cell clones expressed EBNA 1 and LMP 1 and very little of EBNA 2, showing the BamHI F promoter-driven latency II form of infection, which is seen in non-B-cell tumors. This is the first report of in vitro generation of latency II type EBV infection. The present system of persistent EBV infection in T cells should be a good model for investigating the pathogenic role of EBV in non-B-cell tumors. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/7641689/Persistent_Epstein_Barr_virus_infection_in_a_human_T_cell_line:_unique_program_of_latent_virus_expression_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1995&volume=14&issue=15&spage=3706 DB - PRIME DP - Unbound Medicine ER -