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Autoradiographic labelling of P2 purinoceptors in the guinea-pig cochlea.
Hear Res 1995; 84(1-2):177-93HR

Abstract

Two different radioligands were used to identify extracellular ATP binding sites specific to P2 purinoceptors in guinea-pig cochlear tissue. Deoxyadenosine 5'-(alpha-[35S]thio)triphosphate ([35S]dATP alpha S; 10 nM) provided a high activity probe for the P2y purinoceptor subtype on the basis of selective block by 2-methylthio-ATP (2MeSATP; 100 microM). [3H]alpha, beta-methylene-ATP (10 nM), a high affinity probe for a P2x purinoceptor subtype was selectively blocked by inclusion of the related compound beta, gamma-methylene-ATP (100 microM). Both probes labelled the organ of Corti, stria vascularis and spiral prominence regions. The P2x purinoceptor probe also bound to lateral wall tissue below the spiral prominence and insertion point of the basilar membrane within the scala tympani compartment, a region which failed to show significant binding using [35S]dATP alpha S. Frozen sections of whole cochlea permitted analysis of radioligand binding to the cell body region (spiral ganglion in Rosenthal's canal) of the primary auditory afferents and the auditory nerve itself, which lies within the central region of the modiolus of the cochlea. Both these regions exhibited 2MeSATP blockable [35S]dATP alpha S binding whereas specific [3H]alpha, beta-methylene-ATP binding was absent from spiral ganglion and minimal in the auditory nerve region. These results demonstrate a mixed P2 purinoceptor distribution in cochlear tissues and suggest that complex purine-mediated neurohumoral mechanisms may influence cochlear function at a number of sites.

Authors+Show Affiliations

Department of Physiology, School of Medicine, University of Auckland, New Zealand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7642450

Citation

Mockett, B G., et al. "Autoradiographic Labelling of P2 Purinoceptors in the Guinea-pig Cochlea." Hearing Research, vol. 84, no. 1-2, 1995, pp. 177-93.
Mockett BG, Bo X, Housley GD, et al. Autoradiographic labelling of P2 purinoceptors in the guinea-pig cochlea. Hear Res. 1995;84(1-2):177-93.
Mockett, B. G., Bo, X., Housley, G. D., Thorne, P. R., & Burnstock, G. (1995). Autoradiographic labelling of P2 purinoceptors in the guinea-pig cochlea. Hearing Research, 84(1-2), pp. 177-93.
Mockett BG, et al. Autoradiographic Labelling of P2 Purinoceptors in the Guinea-pig Cochlea. Hear Res. 1995;84(1-2):177-93. PubMed PMID: 7642450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoradiographic labelling of P2 purinoceptors in the guinea-pig cochlea. AU - Mockett,B G, AU - Bo,X, AU - Housley,G D, AU - Thorne,P R, AU - Burnstock,G, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 177 EP - 93 JF - Hearing research JO - Hear. Res. VL - 84 IS - 1-2 N2 - Two different radioligands were used to identify extracellular ATP binding sites specific to P2 purinoceptors in guinea-pig cochlear tissue. Deoxyadenosine 5'-(alpha-[35S]thio)triphosphate ([35S]dATP alpha S; 10 nM) provided a high activity probe for the P2y purinoceptor subtype on the basis of selective block by 2-methylthio-ATP (2MeSATP; 100 microM). [3H]alpha, beta-methylene-ATP (10 nM), a high affinity probe for a P2x purinoceptor subtype was selectively blocked by inclusion of the related compound beta, gamma-methylene-ATP (100 microM). Both probes labelled the organ of Corti, stria vascularis and spiral prominence regions. The P2x purinoceptor probe also bound to lateral wall tissue below the spiral prominence and insertion point of the basilar membrane within the scala tympani compartment, a region which failed to show significant binding using [35S]dATP alpha S. Frozen sections of whole cochlea permitted analysis of radioligand binding to the cell body region (spiral ganglion in Rosenthal's canal) of the primary auditory afferents and the auditory nerve itself, which lies within the central region of the modiolus of the cochlea. Both these regions exhibited 2MeSATP blockable [35S]dATP alpha S binding whereas specific [3H]alpha, beta-methylene-ATP binding was absent from spiral ganglion and minimal in the auditory nerve region. These results demonstrate a mixed P2 purinoceptor distribution in cochlear tissues and suggest that complex purine-mediated neurohumoral mechanisms may influence cochlear function at a number of sites. SN - 0378-5955 UR - https://www.unboundmedicine.com/medline/citation/7642450/Autoradiographic_labelling_of_P2_purinoceptors_in_the_guinea_pig_cochlea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-5955(95)00024-X DB - PRIME DP - Unbound Medicine ER -