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Parathyroidectomy does not prevent the renal PTH/PTHrP receptor down-regulation in uremic rats.
Kidney Int 1995; 47(6):1797-805KI

Abstract

In a recent study we demonstrated that the PTH/PTHrP receptor (PTH-R) mRNA was markedly down-regulated in the remnant kidney of uremic rats with severe secondary hyperparathyroidism. Among the factors potentially implicated in this down-regulation, to date only PTH has been demonstrated to modulate PTH-R expression. Here, we examined the effect of thyroparathyroidectomy (TPTX) on the renal expression of PTH-R in rats with normal renal function or with chronic renal failure (CRF) induced by 5/6 nephrectomy. Four groups of rats were studied: control, TPTX, CRF, and CRF + TPTX. Moderate-degree renal failure was documented by mean (+/- SD) creatinine clearances (microliter/min/100 g body wt) of 259 +/- 40 and 212 +/- 45 in CRF and CRF + TPTX rats, compared with 646 +/- 123 and 511 +/- 156 in control and TPTX rats, respectively. Plasma phosphorus, calcitriol, and ionized calcium were significantly lower in CRF and CRF + TPTX than in control animals. Plasma ionized calcium and calcitriol were also lower in TPTX than in control rats. Plasma PTH levels (pg/ml) were increased in CRF rats (41.8 +/- 29.4), and markedly decreased in TPTX (10.1 +/- 7.8) and CRF + TPTX (8.0 +/- 3.8) rats compared with control rats (21.7 +/- 7.5). Northern blot analysis showed that the level of the steady-state PTH-R mRNA in the kidney of CRF and CRF + TPTX rats was markedly decreased compared with that of control rats, the ratios of PTH-R mRNA/beta-actin mRNA being 0.28 +/- 0.04 and 0.27 +/- 0.03 versus 0.54 +/- 0.05, respectively.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

INSERM Unité 90, Hôpital Necker, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7643551

Citation

Ureña, P, et al. "Parathyroidectomy Does Not Prevent the Renal PTH/PTHrP Receptor Down-regulation in Uremic Rats." Kidney International, vol. 47, no. 6, 1995, pp. 1797-805.
Ureña P, Mannstadt M, Hruby M, et al. Parathyroidectomy does not prevent the renal PTH/PTHrP receptor down-regulation in uremic rats. Kidney Int. 1995;47(6):1797-805.
Ureña, P., Mannstadt, M., Hruby, M., Ferreira, A., Schmitt, F., Silve, C., ... Segre, G. V. (1995). Parathyroidectomy does not prevent the renal PTH/PTHrP receptor down-regulation in uremic rats. Kidney International, 47(6), pp. 1797-805.
Ureña P, et al. Parathyroidectomy Does Not Prevent the Renal PTH/PTHrP Receptor Down-regulation in Uremic Rats. Kidney Int. 1995;47(6):1797-805. PubMed PMID: 7643551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parathyroidectomy does not prevent the renal PTH/PTHrP receptor down-regulation in uremic rats. A1 - Ureña,P, AU - Mannstadt,M, AU - Hruby,M, AU - Ferreira,A, AU - Schmitt,F, AU - Silve,C, AU - Ardaillou,R, AU - Lacour,B, AU - Abou-Samra,A B, AU - Segre,G V, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 1797 EP - 805 JF - Kidney international JO - Kidney Int. VL - 47 IS - 6 N2 - In a recent study we demonstrated that the PTH/PTHrP receptor (PTH-R) mRNA was markedly down-regulated in the remnant kidney of uremic rats with severe secondary hyperparathyroidism. Among the factors potentially implicated in this down-regulation, to date only PTH has been demonstrated to modulate PTH-R expression. Here, we examined the effect of thyroparathyroidectomy (TPTX) on the renal expression of PTH-R in rats with normal renal function or with chronic renal failure (CRF) induced by 5/6 nephrectomy. Four groups of rats were studied: control, TPTX, CRF, and CRF + TPTX. Moderate-degree renal failure was documented by mean (+/- SD) creatinine clearances (microliter/min/100 g body wt) of 259 +/- 40 and 212 +/- 45 in CRF and CRF + TPTX rats, compared with 646 +/- 123 and 511 +/- 156 in control and TPTX rats, respectively. Plasma phosphorus, calcitriol, and ionized calcium were significantly lower in CRF and CRF + TPTX than in control animals. Plasma ionized calcium and calcitriol were also lower in TPTX than in control rats. Plasma PTH levels (pg/ml) were increased in CRF rats (41.8 +/- 29.4), and markedly decreased in TPTX (10.1 +/- 7.8) and CRF + TPTX (8.0 +/- 3.8) rats compared with control rats (21.7 +/- 7.5). Northern blot analysis showed that the level of the steady-state PTH-R mRNA in the kidney of CRF and CRF + TPTX rats was markedly decreased compared with that of control rats, the ratios of PTH-R mRNA/beta-actin mRNA being 0.28 +/- 0.04 and 0.27 +/- 0.03 versus 0.54 +/- 0.05, respectively.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0085-2538 UR - https://www.unboundmedicine.com/medline/citation/7643551/Parathyroidectomy_does_not_prevent_the_renal_PTH/PTHrP_receptor_down_regulation_in_uremic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0085-2538(15)59020-0 DB - PRIME DP - Unbound Medicine ER -