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Gas chromatographic-mass spectrometric identification and quantitation of ethylene glycol in serum after derivatization with perfluorooctanoyl chloride: a novel derivative.
J Chromatogr B Biomed Appl. 1995 Apr 07; 666(1):63-70.JC

Abstract

Ethylene glycol poisoning is a common clinical problem and identification as well as quantitation of ethylene glycol in serum is important for medical and legal purposes. Most investigators described determination of ethylene glycol by gas chromatography without derivatization or derivatives forming a molecular ion < 200. We describe a novel derivatization technique of ethylene glycol using perfluorooctanoyl chloride, after extraction from serum using acetone. This derivative has a molecular mass of 854 and produces a base peak at m/z 441 and other diagnostic strong peaks for unambiguous identification. Moreover, this derivative is less volatile and is free from interferences from endogenous serum components. Quantitation can be achieved by using 1,4-butanediol as an internal standard. The assay showed within-run and between-run precision of 7.2% and 8.0%, respectively, and linearity over the serum ethylene glycol concentration range 70-2240 micrograms/ml with a detection limit of 5 micrograms/ml.

Authors+Show Affiliations

Department of Pathology, University of New Mexico, Albuquerque 87106, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7655622

Citation

Dasgupta, A, et al. "Gas Chromatographic-mass Spectrometric Identification and Quantitation of Ethylene Glycol in Serum After Derivatization With Perfluorooctanoyl Chloride: a Novel Derivative." Journal of Chromatography. B, Biomedical Applications, vol. 666, no. 1, 1995, pp. 63-70.
Dasgupta A, Blackwell W, Griego J, et al. Gas chromatographic-mass spectrometric identification and quantitation of ethylene glycol in serum after derivatization with perfluorooctanoyl chloride: a novel derivative. J Chromatogr B, Biomed Appl. 1995;666(1):63-70.
Dasgupta, A., Blackwell, W., Griego, J., & Malik, S. (1995). Gas chromatographic-mass spectrometric identification and quantitation of ethylene glycol in serum after derivatization with perfluorooctanoyl chloride: a novel derivative. Journal of Chromatography. B, Biomedical Applications, 666(1), 63-70.
Dasgupta A, et al. Gas Chromatographic-mass Spectrometric Identification and Quantitation of Ethylene Glycol in Serum After Derivatization With Perfluorooctanoyl Chloride: a Novel Derivative. J Chromatogr B, Biomed Appl. 1995 Apr 7;666(1):63-70. PubMed PMID: 7655622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas chromatographic-mass spectrometric identification and quantitation of ethylene glycol in serum after derivatization with perfluorooctanoyl chloride: a novel derivative. AU - Dasgupta,A, AU - Blackwell,W, AU - Griego,J, AU - Malik,S, PY - 1995/4/7/pubmed PY - 1995/4/7/medline PY - 1995/4/7/entrez SP - 63 EP - 70 JF - Journal of chromatography. B, Biomedical applications JO - J. Chromatogr. B, Biomed. Appl. VL - 666 IS - 1 N2 - Ethylene glycol poisoning is a common clinical problem and identification as well as quantitation of ethylene glycol in serum is important for medical and legal purposes. Most investigators described determination of ethylene glycol by gas chromatography without derivatization or derivatives forming a molecular ion < 200. We describe a novel derivatization technique of ethylene glycol using perfluorooctanoyl chloride, after extraction from serum using acetone. This derivative has a molecular mass of 854 and produces a base peak at m/z 441 and other diagnostic strong peaks for unambiguous identification. Moreover, this derivative is less volatile and is free from interferences from endogenous serum components. Quantitation can be achieved by using 1,4-butanediol as an internal standard. The assay showed within-run and between-run precision of 7.2% and 8.0%, respectively, and linearity over the serum ethylene glycol concentration range 70-2240 micrograms/ml with a detection limit of 5 micrograms/ml. SN - 1572-6495 UR - https://www.unboundmedicine.com/medline/citation/7655622/Gas_chromatographic_mass_spectrometric_identification_and_quantitation_of_ethylene_glycol_in_serum_after_derivatization_with_perfluorooctanoyl_chloride:_a_novel_derivative_ DB - PRIME DP - Unbound Medicine ER -