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Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast.
Genes Dev. 1995 Sep 01; 9(17):2117-30.GD

Abstract

Simultaneous inactivation of pyp1 and pyp2 PTPases in fission yeast leads to aberrant cell morphology and growth arrest. Spontaneous recessive mutations that bypass the requirement for pyp1 and pyp2 and reside in two complementation groups were isolated, sty1 and sty2. sty1- and sty2- mutant cells are substantially delayed in the timing of mitotic initiation. We have isolated the sty1 gene, which encodes a MAP kinase that is closely related to a subfamily of MAP kinases regulated by osmotic stress including Saccharomyces cervisiae HOG1 and human CSBP1. We find that sty2 is allelic to the wis1 MAP kinase kinase and that delta sty1 and delta wis1 cells are unable to grow in high osmolarity medium. Osmotic stress induces both tyrosine phosphorylation of Sty1 and a reduction in cell size at division. Pyp2 associates with and tyrosine dephosphorylates Sty1 in vitro. We find that wis1-dependent induction of pyp2 mRNA is responsible for tyrosine dephosphorylation of Sty1 in vivo on prolonged exposure to osmotic stress. We conclude that Pyp1 and Pyp2 are tyrosine-specific MAP kinase phosphatases that inactivate an osmoregulated MAP kinase, Sty1, which acts downstream of the Wis1 MAP kinase kinase to control cell size at division in fission yeast.

Authors+Show Affiliations

Division of Yeast Genetics, National Institute for Medical Research, Ridgeway, London, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7657164

Citation

Millar, J B., et al. "Pyp1 and Pyp2 PTPases Dephosphorylate an Osmosensing MAP Kinase Controlling Cell Size at Division in Fission Yeast." Genes & Development, vol. 9, no. 17, 1995, pp. 2117-30.
Millar JB, Buck V, Wilkinson MG. Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast. Genes Dev. 1995;9(17):2117-30.
Millar, J. B., Buck, V., & Wilkinson, M. G. (1995). Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast. Genes & Development, 9(17), 2117-30.
Millar JB, Buck V, Wilkinson MG. Pyp1 and Pyp2 PTPases Dephosphorylate an Osmosensing MAP Kinase Controlling Cell Size at Division in Fission Yeast. Genes Dev. 1995 Sep 1;9(17):2117-30. PubMed PMID: 7657164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast. AU - Millar,J B, AU - Buck,V, AU - Wilkinson,M G, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 2117 EP - 30 JF - Genes & development JO - Genes Dev VL - 9 IS - 17 N2 - Simultaneous inactivation of pyp1 and pyp2 PTPases in fission yeast leads to aberrant cell morphology and growth arrest. Spontaneous recessive mutations that bypass the requirement for pyp1 and pyp2 and reside in two complementation groups were isolated, sty1 and sty2. sty1- and sty2- mutant cells are substantially delayed in the timing of mitotic initiation. We have isolated the sty1 gene, which encodes a MAP kinase that is closely related to a subfamily of MAP kinases regulated by osmotic stress including Saccharomyces cervisiae HOG1 and human CSBP1. We find that sty2 is allelic to the wis1 MAP kinase kinase and that delta sty1 and delta wis1 cells are unable to grow in high osmolarity medium. Osmotic stress induces both tyrosine phosphorylation of Sty1 and a reduction in cell size at division. Pyp2 associates with and tyrosine dephosphorylates Sty1 in vitro. We find that wis1-dependent induction of pyp2 mRNA is responsible for tyrosine dephosphorylation of Sty1 in vivo on prolonged exposure to osmotic stress. We conclude that Pyp1 and Pyp2 are tyrosine-specific MAP kinase phosphatases that inactivate an osmoregulated MAP kinase, Sty1, which acts downstream of the Wis1 MAP kinase kinase to control cell size at division in fission yeast. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/7657164/Pyp1_and_Pyp2_PTPases_dephosphorylate_an_osmosensing_MAP_kinase_controlling_cell_size_at_division_in_fission_yeast_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=7657164 DB - PRIME DP - Unbound Medicine ER -