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Protective action of zinc against glutamate neurotoxicity in cultured retinal neurons.
Invest Ophthalmol Vis Sci. 1995 Sep; 36(10):2048-53.IO

Abstract

PURPOSE

To examine the effects of Zn2+ on glutamate-induced neurotoxicity in cultured retinal neurons.

METHODS

Primary cultures obtained from fetal rat retinas (16 to 19 days gestation) were used. The neurotoxic effects of excitatory amino acids were quantitatively assessed using the trypan blue exclusion method.

RESULTS

A brief exposure of retinal cultures to glutamate or N-methyl-D-aspartate (NMDA) induced delayed cell death. Zn2+ at concentrations of 3 to 30 microM ameliorated glutamate- and NMDA-induced neurotoxicity in a dose-dependent manner. By contrast, neurotoxicity induced by a 1-hour exposure to kainate was not affected by Zn2+.

CONCLUSIONS

These findings demonstrate that Zn2+ protects retinal neurons from NMDA receptor-mediated glutamate neurotoxicity.

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Publisher Full Text

Authors+Show Affiliations

Kikuchi M
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Kashii S
No affiliation info available
Honda Y
No affiliation info available
Ujihara H
No affiliation info available
Sasa M
No affiliation info available
Tamura Y
No affiliation info available
Akaike A
No affiliation info available

MeSH

AnimalsCell SurvivalCells, CulturedChloridesDose-Response Relationship, DrugFetusGlutamic AcidKainic AcidN-MethylaspartateNeuronsRatsReceptors, GlutamateRetinaZinc Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7657543

Citation

Kikuchi, M, et al. "Protective Action of Zinc Against Glutamate Neurotoxicity in Cultured Retinal Neurons." Investigative Ophthalmology & Visual Science, vol. 36, no. 10, 1995, pp. 2048-53.
Kikuchi M, Kashii S, Honda Y, et al. Protective action of zinc against glutamate neurotoxicity in cultured retinal neurons. Invest Ophthalmol Vis Sci. 1995;36(10):2048-53.
Kikuchi, M., Kashii, S., Honda, Y., Ujihara, H., Sasa, M., Tamura, Y., & Akaike, A. (1995). Protective action of zinc against glutamate neurotoxicity in cultured retinal neurons. Investigative Ophthalmology & Visual Science, 36(10), 2048-53.
Kikuchi M, et al. Protective Action of Zinc Against Glutamate Neurotoxicity in Cultured Retinal Neurons. Invest Ophthalmol Vis Sci. 1995;36(10):2048-53. PubMed PMID: 7657543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective action of zinc against glutamate neurotoxicity in cultured retinal neurons. AU - Kikuchi,M, AU - Kashii,S, AU - Honda,Y, AU - Ujihara,H, AU - Sasa,M, AU - Tamura,Y, AU - Akaike,A, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 2048 EP - 53 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 36 IS - 10 N2 - PURPOSE: To examine the effects of Zn2+ on glutamate-induced neurotoxicity in cultured retinal neurons. METHODS: Primary cultures obtained from fetal rat retinas (16 to 19 days gestation) were used. The neurotoxic effects of excitatory amino acids were quantitatively assessed using the trypan blue exclusion method. RESULTS: A brief exposure of retinal cultures to glutamate or N-methyl-D-aspartate (NMDA) induced delayed cell death. Zn2+ at concentrations of 3 to 30 microM ameliorated glutamate- and NMDA-induced neurotoxicity in a dose-dependent manner. By contrast, neurotoxicity induced by a 1-hour exposure to kainate was not affected by Zn2+. CONCLUSIONS: These findings demonstrate that Zn2+ protects retinal neurons from NMDA receptor-mediated glutamate neurotoxicity. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/7657543/Protective_action_of_zinc_against_glutamate_neurotoxicity_in_cultured_retinal_neurons_ L2 - https://iovs.arvojournals.org/article.aspx?volume=36&issue=10&page=2048 DB - PRIME DP - Unbound Medicine ER -