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Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sézary syndrome.
Arch Dermatol. 1995 Sep; 131(9):1027-31.AD

Abstract

BACKGROUND AND DESIGN

We used the amplification of junctional V (variable)-J joining sequences of the rearranged T-cell receptor gamma (TCR gamma) genes by polymerase chain reaction for rapid and sensitive detection of a clonal T-cell population in a total of 51 skin specimens obtained from 45 patients with mycosis fungoides, five patients with Sézary syndrome, and 29 patients with chronic inflammatory dermatoses.

RESULTS

A clonal TCR gamma gene rearrangement was present in all tumors (3/3, 100%) and in most infiltrated plaques (16/22, 73%) and erythrodermas (10/12, 83%). In the patch stage, a clonal subset was found in more than half of the cases (8/14, 57%), whereas no clonality was observed in the controls. We also amplified the V-J sequences of the Igh locus coding for the heavy chain of immunoglobulins, without evidence of clonal rearrangement. These data were compared with those from in situ immunophenotypic analysis. Moreover, by using the same assay with successive dilutions of standard clonal T-cell DNA, a semiquantitative study of the T-cell clone was carried out in some cases. The highest ratios of clonal DNA were observed in advanced stages.

CONCLUSIONS

These data validate polymerase chain reaction V gamma-J gamma as a rapid, sensitive tool that can be used in the routine analysis of clonality in cutaneous lesions of mycosis fungoides and in the early diagnosis of mycosis fungoides and Sézary syndrome. Semiquantitative studies suggest that the malignant T-cell clone follows a selective process during the course of the progressive form of mycosis fungoides.

Authors+Show Affiliations

Clinique des Maladies Cutanées, Hôpital Saint-Louis, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7661604

Citation

Bachelez, H, et al. "Detection of Clonal T-cell Receptor Gamma Gene Rearrangements With the Use of the Polymerase Chain Reaction in Cutaneous Lesions of Mycosis Fungoides and Sézary Syndrome." Archives of Dermatology, vol. 131, no. 9, 1995, pp. 1027-31.
Bachelez H, Bioul L, Flageul B, et al. Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sézary syndrome. Arch Dermatol. 1995;131(9):1027-31.
Bachelez, H., Bioul, L., Flageul, B., Baccard, M., Moulonguet-Michau, I., Verola, O., Morel, P., Dubertret, L., & Sigaux, F. (1995). Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sézary syndrome. Archives of Dermatology, 131(9), 1027-31.
Bachelez H, et al. Detection of Clonal T-cell Receptor Gamma Gene Rearrangements With the Use of the Polymerase Chain Reaction in Cutaneous Lesions of Mycosis Fungoides and Sézary Syndrome. Arch Dermatol. 1995;131(9):1027-31. PubMed PMID: 7661604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sézary syndrome. AU - Bachelez,H, AU - Bioul,L, AU - Flageul,B, AU - Baccard,M, AU - Moulonguet-Michau,I, AU - Verola,O, AU - Morel,P, AU - Dubertret,L, AU - Sigaux,F, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 1027 EP - 31 JF - Archives of dermatology JO - Arch Dermatol VL - 131 IS - 9 N2 - BACKGROUND AND DESIGN: We used the amplification of junctional V (variable)-J joining sequences of the rearranged T-cell receptor gamma (TCR gamma) genes by polymerase chain reaction for rapid and sensitive detection of a clonal T-cell population in a total of 51 skin specimens obtained from 45 patients with mycosis fungoides, five patients with Sézary syndrome, and 29 patients with chronic inflammatory dermatoses. RESULTS: A clonal TCR gamma gene rearrangement was present in all tumors (3/3, 100%) and in most infiltrated plaques (16/22, 73%) and erythrodermas (10/12, 83%). In the patch stage, a clonal subset was found in more than half of the cases (8/14, 57%), whereas no clonality was observed in the controls. We also amplified the V-J sequences of the Igh locus coding for the heavy chain of immunoglobulins, without evidence of clonal rearrangement. These data were compared with those from in situ immunophenotypic analysis. Moreover, by using the same assay with successive dilutions of standard clonal T-cell DNA, a semiquantitative study of the T-cell clone was carried out in some cases. The highest ratios of clonal DNA were observed in advanced stages. CONCLUSIONS: These data validate polymerase chain reaction V gamma-J gamma as a rapid, sensitive tool that can be used in the routine analysis of clonality in cutaneous lesions of mycosis fungoides and in the early diagnosis of mycosis fungoides and Sézary syndrome. Semiquantitative studies suggest that the malignant T-cell clone follows a selective process during the course of the progressive form of mycosis fungoides. SN - 0003-987X UR - https://www.unboundmedicine.com/medline/citation/7661604/Detection_of_clonal_T_cell_receptor_gamma_gene_rearrangements_with_the_use_of_the_polymerase_chain_reaction_in_cutaneous_lesions_of_mycosis_fungoides_and_Sézary_syndrome_ DB - PRIME DP - Unbound Medicine ER -