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Identification of microsporidia in stool specimens by using PCR and restriction endonucleases.
J Clin Microbiol. 1995 Jul; 33(7):1739-41.JC

Abstract

We report the development of a PCR-based assay for the detection of microsporidia in clinical specimens. A single primer pair complementary to conserved sequences of the small-subunit rRNA enabled amplification of DNA from the four major microsporidian pathogens of humans: Encephalitozoon cuniculi, Encephalitozoon hellem, Enterocytozoon bieneusi, and Septata intestinalis. The extraction method allowed PCR amplification of E. bieneusi and S. intestinalis DNA from sodium hypochlorite-treated stool specimens. Differentiation of the microsporidian gastrointestinal pathogens E. bieneusi and S. intestinalis could be accomplished by restriction endonuclease digestion of PCR products using PstI and HaeIII.

Authors+Show Affiliations

Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7665639

Citation

Fedorko, D P., et al. "Identification of Microsporidia in Stool Specimens By Using PCR and Restriction Endonucleases." Journal of Clinical Microbiology, vol. 33, no. 7, 1995, pp. 1739-41.
Fedorko DP, Nelson NA, Cartwright CP. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. J Clin Microbiol. 1995;33(7):1739-41.
Fedorko, D. P., Nelson, N. A., & Cartwright, C. P. (1995). Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. Journal of Clinical Microbiology, 33(7), 1739-41.
Fedorko DP, Nelson NA, Cartwright CP. Identification of Microsporidia in Stool Specimens By Using PCR and Restriction Endonucleases. J Clin Microbiol. 1995;33(7):1739-41. PubMed PMID: 7665639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. AU - Fedorko,D P, AU - Nelson,N A, AU - Cartwright,C P, PY - 1995/7/1/pubmed PY - 1995/7/1/medline PY - 1995/7/1/entrez SP - 1739 EP - 41 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 33 IS - 7 N2 - We report the development of a PCR-based assay for the detection of microsporidia in clinical specimens. A single primer pair complementary to conserved sequences of the small-subunit rRNA enabled amplification of DNA from the four major microsporidian pathogens of humans: Encephalitozoon cuniculi, Encephalitozoon hellem, Enterocytozoon bieneusi, and Septata intestinalis. The extraction method allowed PCR amplification of E. bieneusi and S. intestinalis DNA from sodium hypochlorite-treated stool specimens. Differentiation of the microsporidian gastrointestinal pathogens E. bieneusi and S. intestinalis could be accomplished by restriction endonuclease digestion of PCR products using PstI and HaeIII. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7665639/Identification_of_microsporidia_in_stool_specimens_by_using_PCR_and_restriction_endonucleases_ L2 - https://journals.asm.org/doi/10.1128/jcm.33.7.1739-1741.1995?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -