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The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections.
J Med Vet Mycol. 1995 May-Jun; 33(3):167-70.JM

Abstract

To understand the significance of proteolytic enzymes in the pathogenesis of dermatophyte infections, we have studied the expression of proteolytic activity in vitro. Cultures were extracted after 1, 2, 3, 4 and 5 weeks of growth and the proteolytic activity measured by a peptide release assay using human stratum corneum as a substrate. Maximum activity was noted at 3 weeks which corresponded to the dissolution of the stratum corneum particles. Isolates of Trichophyton mentagrophytes and Trichophyton rubrum from 42 patients with tinea pedis or tinea unguium were established on 2% glucose peptone plates. Using the peptide release assay it was found that after 3 weeks culture, T. mentagrophytes showed higher activity that T. rubrum in tinea pedis (2044 +/- 1067 micrograms protein released h-1 mg-1, n = 13 compared to 828 +/- 614 micrograms protein released h-1 mg-1, n = 13). In tinea unguium, the values at 3 weeks were generally lower. Trichophyton mentagrophytes activity was 837 +/- 121 micrograms protein released h-1 mg-1 (n = 3) compared to 470 +/- 271 micrograms protein released h-1 mg-1 (n = 13) for T. rubrum. Thus, as well as a variation in activity between the two species, there appears to be a variation in site in terms of expression of activity.

Authors+Show Affiliations

Department of Dermatology, University of Wales College of Medicine, Cardiff, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7666296

Citation

Samdani, A J., et al. "The Proteolytic Activity of Strains of T. Mentagrophytes and T. Rubrum Isolated From Tinea Pedis and Tinea Unguium Infections." Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology, vol. 33, no. 3, 1995, pp. 167-70.
Samdani AJ, Dykes PJ, Marks R. The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections. J Med Vet Mycol. 1995;33(3):167-70.
Samdani, A. J., Dykes, P. J., & Marks, R. (1995). The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections. Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology, 33(3), 167-70.
Samdani AJ, Dykes PJ, Marks R. The Proteolytic Activity of Strains of T. Mentagrophytes and T. Rubrum Isolated From Tinea Pedis and Tinea Unguium Infections. J Med Vet Mycol. 1995 May-Jun;33(3):167-70. PubMed PMID: 7666296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections. AU - Samdani,A J, AU - Dykes,P J, AU - Marks,R, PY - 1995/5/1/pubmed PY - 1995/5/1/medline PY - 1995/5/1/entrez SP - 167 EP - 70 JF - Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology JO - J. Med. Vet. Mycol. VL - 33 IS - 3 N2 - To understand the significance of proteolytic enzymes in the pathogenesis of dermatophyte infections, we have studied the expression of proteolytic activity in vitro. Cultures were extracted after 1, 2, 3, 4 and 5 weeks of growth and the proteolytic activity measured by a peptide release assay using human stratum corneum as a substrate. Maximum activity was noted at 3 weeks which corresponded to the dissolution of the stratum corneum particles. Isolates of Trichophyton mentagrophytes and Trichophyton rubrum from 42 patients with tinea pedis or tinea unguium were established on 2% glucose peptone plates. Using the peptide release assay it was found that after 3 weeks culture, T. mentagrophytes showed higher activity that T. rubrum in tinea pedis (2044 +/- 1067 micrograms protein released h-1 mg-1, n = 13 compared to 828 +/- 614 micrograms protein released h-1 mg-1, n = 13). In tinea unguium, the values at 3 weeks were generally lower. Trichophyton mentagrophytes activity was 837 +/- 121 micrograms protein released h-1 mg-1 (n = 3) compared to 470 +/- 271 micrograms protein released h-1 mg-1 (n = 13) for T. rubrum. Thus, as well as a variation in activity between the two species, there appears to be a variation in site in terms of expression of activity. SN - 0268-1218 UR - https://www.unboundmedicine.com/medline/citation/7666296/The_proteolytic_activity_of_strains_of_T__mentagrophytes_and_T__rubrum_isolated_from_tinea_pedis_and_tinea_unguium_infections_ L2 - https://medlineplus.gov/athletesfoot.html DB - PRIME DP - Unbound Medicine ER -