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Combined histochemical stains in the differential diagnosis of Cryptococcus neoformans.
Mod Pathol. 1993 Jan; 6(1):80-4.MP

Abstract

Three combinations of histochemical stains were used to study 69 routinely processed tissues containing various "yeast-like" fungal organisms (Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, Paracoccidioides brasiliensis, and Candida albicans). Fontana-Masson stain combined either with mucicarmine or Alcian blue produced distinctive staining of C. neoformans (Fontana-Masson-positive wall and mucin-positive capsule) that was not identified in other fungi. The Alcian blue stain combined with periodic acid-Schiff reaction also was useful for fungal classification, because the Alcian blue component reacted with only C. neoformans (capsule) and B. dermatitidis (wall) and the periodic acid-Schiff reaction reacted with the cell wall and body of each fungus. We concluded that the combined histochemical stains used in conjunction with morphologic study of hematoxylin and eosin- and Gomori methenamine silver-stained sections are helpful in the differential diagnosis of yeast-like fungal organisms.

Authors+Show Affiliations

Lazcano O
Section of Surgical Pathology, Mayo Clinic, Rochester, Minnesota.
Speights VO
No affiliation info available
Strickler JG
No affiliation info available
Bilbao JE
No affiliation info available
Becker J
No affiliation info available
Diaz J
No affiliation info available

MeSH

Alcian BlueCarmineColoring AgentsCryptococcus neoformansDiagnosis, DifferentialHistocytochemistryHumansMitosporic FungiPeriodic Acid-Schiff ReactionStaining and Labeling

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7678937

Citation

Lazcano, O, et al. "Combined Histochemical Stains in the Differential Diagnosis of Cryptococcus Neoformans." Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc, vol. 6, no. 1, 1993, pp. 80-4.
Lazcano O, Speights VO, Strickler JG, et al. Combined histochemical stains in the differential diagnosis of Cryptococcus neoformans. Mod Pathol. 1993;6(1):80-4.
Lazcano, O., Speights, V. O., Strickler, J. G., Bilbao, J. E., Becker, J., & Diaz, J. (1993). Combined histochemical stains in the differential diagnosis of Cryptococcus neoformans. Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc, 6(1), 80-4.
Lazcano O, et al. Combined Histochemical Stains in the Differential Diagnosis of Cryptococcus Neoformans. Mod Pathol. 1993;6(1):80-4. PubMed PMID: 7678937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined histochemical stains in the differential diagnosis of Cryptococcus neoformans. AU - Lazcano,O, AU - Speights,V O,Jr AU - Strickler,J G, AU - Bilbao,J E, AU - Becker,J, AU - Diaz,J, PY - 1993/1/1/pubmed PY - 2001/3/28/medline PY - 1993/1/1/entrez SP - 80 EP - 4 JF - Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc JO - Mod Pathol VL - 6 IS - 1 N2 - Three combinations of histochemical stains were used to study 69 routinely processed tissues containing various "yeast-like" fungal organisms (Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, Paracoccidioides brasiliensis, and Candida albicans). Fontana-Masson stain combined either with mucicarmine or Alcian blue produced distinctive staining of C. neoformans (Fontana-Masson-positive wall and mucin-positive capsule) that was not identified in other fungi. The Alcian blue stain combined with periodic acid-Schiff reaction also was useful for fungal classification, because the Alcian blue component reacted with only C. neoformans (capsule) and B. dermatitidis (wall) and the periodic acid-Schiff reaction reacted with the cell wall and body of each fungus. We concluded that the combined histochemical stains used in conjunction with morphologic study of hematoxylin and eosin- and Gomori methenamine silver-stained sections are helpful in the differential diagnosis of yeast-like fungal organisms. SN - 0893-3952 UR - https://www.unboundmedicine.com/medline/citation/7678937/Combined_histochemical_stains_in_the_differential_diagnosis_of_Cryptococcus_neoformans_ DB - PRIME DP - Unbound Medicine ER -