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Activation of the Na+/K(+)-pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery.
Br J Pharmacol. 1993 Jan; 108(1):120-5.BJ

Abstract

1. The activity of the Na+/K(+)-pump in rat peritoneal mast cells was measured at various time intervals after induction of cellular histamine release by compound 48/80 or by the antigen-antibody reaction. The Na+/K(+)-pump activity was assessed as the ouabain-sensitive potassium uptake of the cells using 86Rb+ as a tracer for potassium (K+(86Rb+)-uptake). 2. Stimulation of the cells with compound 48/80 induced a time and concentration dependent increase of the Na+/K(+)-pump activity. The pump activity was maximal 2 min after stimulation of the cells. Then, the activity gradually decreased and reached a level not significantly different from the controls after 2 h of incubation. 3. When the cells were stimulated by the antigen-antibody reaction, there was also a rapid (within 5 min) stimulation of the Na+/K(+)-pump. In contrast to the stimulation with compound 48/80, the pump activity returned to the control level after 60 min of incubation with antigen. 4. The ouabain-resistant potassium uptake of the cells was increased after stimulation of the cells, regardless of the secretagogue used. This probably reflects the increased surface area of the cells present after the histamine release. 5. On the basis of the present results, we suggest a role for the Na+/K(+)-pump in the recovery process of the mast cell following histamine release.

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Authors+Show Affiliations

Knudsen T
Department of Pharmacology, Odense University, Denmark.
Ferjan I
No affiliation info available
Johansen T
No affiliation info available

MeSH

AnimalsAntigen-Antibody ReactionsCopperHistamine ReleaseMaleMast CellsOuabainPeritoneal CavityPotassiumRatsRats, Sprague-DawleyRats, WistarSodium-Potassium-Exchanging ATPasep-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7679025

Citation

Knudsen, T, et al. "Activation of the Na+/K(+)-pump in Rat Peritoneal Mast Cells Following Histamine Release: a Possible Role in Cell Recovery." British Journal of Pharmacology, vol. 108, no. 1, 1993, pp. 120-5.
Knudsen T, Ferjan I, Johansen T. Activation of the Na+/K(+)-pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery. Br J Pharmacol. 1993;108(1):120-5.
Knudsen, T., Ferjan, I., & Johansen, T. (1993). Activation of the Na+/K(+)-pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery. British Journal of Pharmacology, 108(1), 120-5.
Knudsen T, Ferjan I, Johansen T. Activation of the Na+/K(+)-pump in Rat Peritoneal Mast Cells Following Histamine Release: a Possible Role in Cell Recovery. Br J Pharmacol. 1993;108(1):120-5. PubMed PMID: 7679025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the Na+/K(+)-pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery. AU - Knudsen,T, AU - Ferjan,I, AU - Johansen,T, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 120 EP - 5 JF - British journal of pharmacology JO - Br J Pharmacol VL - 108 IS - 1 N2 - 1. The activity of the Na+/K(+)-pump in rat peritoneal mast cells was measured at various time intervals after induction of cellular histamine release by compound 48/80 or by the antigen-antibody reaction. The Na+/K(+)-pump activity was assessed as the ouabain-sensitive potassium uptake of the cells using 86Rb+ as a tracer for potassium (K+(86Rb+)-uptake). 2. Stimulation of the cells with compound 48/80 induced a time and concentration dependent increase of the Na+/K(+)-pump activity. The pump activity was maximal 2 min after stimulation of the cells. Then, the activity gradually decreased and reached a level not significantly different from the controls after 2 h of incubation. 3. When the cells were stimulated by the antigen-antibody reaction, there was also a rapid (within 5 min) stimulation of the Na+/K(+)-pump. In contrast to the stimulation with compound 48/80, the pump activity returned to the control level after 60 min of incubation with antigen. 4. The ouabain-resistant potassium uptake of the cells was increased after stimulation of the cells, regardless of the secretagogue used. This probably reflects the increased surface area of the cells present after the histamine release. 5. On the basis of the present results, we suggest a role for the Na+/K(+)-pump in the recovery process of the mast cell following histamine release. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/7679025/Activation_of_the_Na+/K_+__pump_in_rat_peritoneal_mast_cells_following_histamine_release:_a_possible_role_in_cell_recovery_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1993&volume=108&issue=1&spage=120 DB - PRIME DP - Unbound Medicine ER -