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Nonadrenergic noncholinergic neurotransmitter of feline trachealis: VIP or nitric oxide?
J Appl Physiol (1985). 1993 Jan; 74(1):31-9.JA

Abstract

We tested the hypothesis that vasoactive intestinal peptide (VIP) or nitric oxide (NO) is the nonadrenergic noncholinergic (NANC) neurotransmitter in feline trachealis. Isometric tension was measured in trachealis (open or closed tracheal rings) in vitro. Propranolol (10 microM) and atropine (1 microM) were present throughout the experiment, and smooth muscle tone was increased to 60-90% maximal with 5-hydroxytryptamine. We used three methodologies to reduce the relaxation function of VIP, which in turn should reduce NANC-mediated relaxation. 1) The putative VIP antagonist peptide T (10 microM) did not affect VIP concentration-response curves or electrical field stimulation- (EFS) induced NANC responses. 2) Incubation of tissue in specific VIP antiserum (16 h at 4 degrees C) did not reduce EFS-induced NANC relaxations relative to tissue incubated in normal rabbit serum (P > 0.05). On the basis of our passive immunization techniques, it is not possible to absolutely reject VIP as the NANC transmitter. We speculate that nonspecific peptidases present in normal serum and VIP antiserum reduce EFS-induced responses similarly. 3) VIP desensitization, confirmed by a significant rightward shift (P < 0.01) in the VIP concentration-response curve, was achieved by exposing tissues (n = 11) to 1.0 microM VIP for 30 min. Desensitization did not reduce the EFS-induced NANC relaxatory response (P < 0.05) compared with control tissues, suggesting that VIP is not the NANC mediator.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Physiology, Queen's University, Kingston, Ontario, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7680336

Citation

Fisher, J T., et al. "Nonadrenergic Noncholinergic Neurotransmitter of Feline Trachealis: VIP or Nitric Oxide?" Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 74, no. 1, 1993, pp. 31-9.
Fisher JT, Anderson JW, Waldron MA. Nonadrenergic noncholinergic neurotransmitter of feline trachealis: VIP or nitric oxide? J Appl Physiol. 1993;74(1):31-9.
Fisher, J. T., Anderson, J. W., & Waldron, M. A. (1993). Nonadrenergic noncholinergic neurotransmitter of feline trachealis: VIP or nitric oxide? Journal of Applied Physiology (Bethesda, Md. : 1985), 74(1), 31-9.
Fisher JT, Anderson JW, Waldron MA. Nonadrenergic Noncholinergic Neurotransmitter of Feline Trachealis: VIP or Nitric Oxide. J Appl Physiol. 1993;74(1):31-9. PubMed PMID: 7680336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonadrenergic noncholinergic neurotransmitter of feline trachealis: VIP or nitric oxide? AU - Fisher,J T, AU - Anderson,J W, AU - Waldron,M A, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 31 EP - 9 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J. Appl. Physiol. VL - 74 IS - 1 N2 - We tested the hypothesis that vasoactive intestinal peptide (VIP) or nitric oxide (NO) is the nonadrenergic noncholinergic (NANC) neurotransmitter in feline trachealis. Isometric tension was measured in trachealis (open or closed tracheal rings) in vitro. Propranolol (10 microM) and atropine (1 microM) were present throughout the experiment, and smooth muscle tone was increased to 60-90% maximal with 5-hydroxytryptamine. We used three methodologies to reduce the relaxation function of VIP, which in turn should reduce NANC-mediated relaxation. 1) The putative VIP antagonist peptide T (10 microM) did not affect VIP concentration-response curves or electrical field stimulation- (EFS) induced NANC responses. 2) Incubation of tissue in specific VIP antiserum (16 h at 4 degrees C) did not reduce EFS-induced NANC relaxations relative to tissue incubated in normal rabbit serum (P > 0.05). On the basis of our passive immunization techniques, it is not possible to absolutely reject VIP as the NANC transmitter. We speculate that nonspecific peptidases present in normal serum and VIP antiserum reduce EFS-induced responses similarly. 3) VIP desensitization, confirmed by a significant rightward shift (P < 0.01) in the VIP concentration-response curve, was achieved by exposing tissues (n = 11) to 1.0 microM VIP for 30 min. Desensitization did not reduce the EFS-induced NANC relaxatory response (P < 0.05) compared with control tissues, suggesting that VIP is not the NANC mediator.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/7680336/Nonadrenergic_noncholinergic_neurotransmitter_of_feline_trachealis:_VIP_or_nitric_oxide L2 - http://www.physiology.org/doi/full/10.1152/jappl.1993.74.1.31?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -