Tags

Type your tag names separated by a space and hit enter

Recombinant human insulin-like growth factor binding protein-3 (rhIGFBP-3): effects on the glycemic and growth promoting activities of rhIGF-1 in the rat.
Growth Regul. 1993 Mar; 3(1):50-2.GR

Abstract

The effects of IGFBPs on IGF-1 bioactivity in vivo remain largely unknown. We have tested the ability of rhIGFBP-3, made in 293 cells or CHO cells, to affect the glycemic and anabolic activities of co-administered rhIGF-1. For glycemic studies female dw/dw rats were anesthetized, a jugular catheter inserted, basal blood samples taken and i.v. injections given of rhIGF-1 (0.25 mg/kg), rhIGFBP-3 (0.50 mg/kg), or rhIGF-1 plus rhIGFBP-3 (0.25 plus 0.50 mg/kg, respectively). The blood glucose (20 min later, as a percent of initial, mean +/- SD) reached its nadir for rhIGF-1 alone (58 +/- 5%) but was not changed by rhIGFBP-3 alone (99 +/- 4%) or rhIGF-1 plus rhIGFBP-3 (93 +/- 5%). In growth studies, young female hypophysectomized rats (90-105 g) were injected s.c. twice daily for 3-4 days, or infused s.c. for 7 days, with excipient, rhIGF-1 or rhIGF-1 plus rhIGFBP-3 at doses similar to those used in the hypoglycemia studies. Weight gain induced by rhIGF-1 was either unchanged, or enhanced, when the rhIGF-1 was delivered with rhIGFBP-3. The hypoglycemic activity of IGF-1 was greatly reduced if IGF-1 was administered bound to 293-cell rhIGFBP-3 but anabolic activity was unchanged or enhanced.

Authors+Show Affiliations

Genentech, Inc., South San Francisco, CA 94080.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7683530

Citation

Clark, R G., et al. "Recombinant Human Insulin-like Growth Factor Binding Protein-3 (rhIGFBP-3): Effects On the Glycemic and Growth Promoting Activities of rhIGF-1 in the Rat." Growth Regulation, vol. 3, no. 1, 1993, pp. 50-2.
Clark RG, Mortensen D, Reifsynder D, et al. Recombinant human insulin-like growth factor binding protein-3 (rhIGFBP-3): effects on the glycemic and growth promoting activities of rhIGF-1 in the rat. Growth Regul. 1993;3(1):50-2.
Clark, R. G., Mortensen, D., Reifsynder, D., Mohler, M., Etcheverry, T., & Mukku, V. (1993). Recombinant human insulin-like growth factor binding protein-3 (rhIGFBP-3): effects on the glycemic and growth promoting activities of rhIGF-1 in the rat. Growth Regulation, 3(1), 50-2.
Clark RG, et al. Recombinant Human Insulin-like Growth Factor Binding Protein-3 (rhIGFBP-3): Effects On the Glycemic and Growth Promoting Activities of rhIGF-1 in the Rat. Growth Regul. 1993;3(1):50-2. PubMed PMID: 7683530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant human insulin-like growth factor binding protein-3 (rhIGFBP-3): effects on the glycemic and growth promoting activities of rhIGF-1 in the rat. AU - Clark,R G, AU - Mortensen,D, AU - Reifsynder,D, AU - Mohler,M, AU - Etcheverry,T, AU - Mukku,V, PY - 1993/3/1/pubmed PY - 1993/3/1/medline PY - 1993/3/1/entrez SP - 50 EP - 2 JF - Growth regulation JO - Growth Regul VL - 3 IS - 1 N2 - The effects of IGFBPs on IGF-1 bioactivity in vivo remain largely unknown. We have tested the ability of rhIGFBP-3, made in 293 cells or CHO cells, to affect the glycemic and anabolic activities of co-administered rhIGF-1. For glycemic studies female dw/dw rats were anesthetized, a jugular catheter inserted, basal blood samples taken and i.v. injections given of rhIGF-1 (0.25 mg/kg), rhIGFBP-3 (0.50 mg/kg), or rhIGF-1 plus rhIGFBP-3 (0.25 plus 0.50 mg/kg, respectively). The blood glucose (20 min later, as a percent of initial, mean +/- SD) reached its nadir for rhIGF-1 alone (58 +/- 5%) but was not changed by rhIGFBP-3 alone (99 +/- 4%) or rhIGF-1 plus rhIGFBP-3 (93 +/- 5%). In growth studies, young female hypophysectomized rats (90-105 g) were injected s.c. twice daily for 3-4 days, or infused s.c. for 7 days, with excipient, rhIGF-1 or rhIGF-1 plus rhIGFBP-3 at doses similar to those used in the hypoglycemia studies. Weight gain induced by rhIGF-1 was either unchanged, or enhanced, when the rhIGF-1 was delivered with rhIGFBP-3. The hypoglycemic activity of IGF-1 was greatly reduced if IGF-1 was administered bound to 293-cell rhIGFBP-3 but anabolic activity was unchanged or enhanced. SN - 0956-523X UR - https://www.unboundmedicine.com/medline/citation/7683530/Recombinant_human_insulin_like_growth_factor_binding_protein_3__rhIGFBP_3_:_effects_on_the_glycemic_and_growth_promoting_activities_of_rhIGF_1_in_the_rat_ L2 - https://medlineplus.gov/bloodsugar.html DB - PRIME DP - Unbound Medicine ER -