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[Detection of Pneumocystis carinii in clinical samples by the polymerase chain reaction. Comparison with the methenamine silver technique].
Enferm Infecc Microbiol Clin. 1993 Mar; 11(3):143-6.EI

Abstract

BACKGROUND

A comparison of polymerase chain reaction (PCR) with methenamine silver stain was performed on clinical specimens for diagnosis of Pneumocystis carinii pneumonia in immunosuppressed patients.

METHODS

25 clinical samples (10 induced sputa, 12 transthoracic needle aspirations and 2 bronchoalveolar lavages) from 17 patients with AIDS and respiratory symptoms were studied. We performed DNA amplification by using 2 primers that amplify the mitochondrial large rDNA sequence of P. carinii.

RESULTS

The sensitivity and specificity of the PCR technique were 92.8% and 100% respectively, with a positive predictive value of 100% and negative predictive value of 92.3%. The sensitivity of the silver stain were 65%, specificity 100%, positive predictive value 100% and negative predictive value 63%.

CONCLUSION

The PCR technique is more sensitive than the methenamine silver stain for detecting Pneumocystis carinii in respiratory specimens from patients with pneumonia. Consequently it could be a very good alternative to classical methods in diagnosis of P. carinii infections.

Authors+Show Affiliations

Servicio de Análisis Clínicos y Microbiología, Hospital Arnau de Vilanova, Universidad de Lérida.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

spa

PubMed ID

7684613

Citation

Nogués, A, et al. "[Detection of Pneumocystis Carinii in Clinical Samples By the Polymerase Chain Reaction. Comparison With the Methenamine Silver Technique]." Enfermedades Infecciosas Y Microbiologia Clinica, vol. 11, no. 3, 1993, pp. 143-6.
Nogués A, García M, Rivas C, et al. [Detection of Pneumocystis carinii in clinical samples by the polymerase chain reaction. Comparison with the methenamine silver technique]. Enferm Infecc Microbiol Clin. 1993;11(3):143-6.
Nogués, A., García, M., Rivas, C., Falguera, M., Puig, T., & Ruiz, A. (1993). [Detection of Pneumocystis carinii in clinical samples by the polymerase chain reaction. Comparison with the methenamine silver technique]. Enfermedades Infecciosas Y Microbiologia Clinica, 11(3), 143-6.
Nogués A, et al. [Detection of Pneumocystis Carinii in Clinical Samples By the Polymerase Chain Reaction. Comparison With the Methenamine Silver Technique]. Enferm Infecc Microbiol Clin. 1993;11(3):143-6. PubMed PMID: 7684613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of Pneumocystis carinii in clinical samples by the polymerase chain reaction. Comparison with the methenamine silver technique]. AU - Nogués,A, AU - García,M, AU - Rivas,C, AU - Falguera,M, AU - Puig,T, AU - Ruiz,A, PY - 1993/3/1/pubmed PY - 1993/3/1/medline PY - 1993/3/1/entrez SP - 143 EP - 6 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm Infecc Microbiol Clin VL - 11 IS - 3 N2 - BACKGROUND: A comparison of polymerase chain reaction (PCR) with methenamine silver stain was performed on clinical specimens for diagnosis of Pneumocystis carinii pneumonia in immunosuppressed patients. METHODS: 25 clinical samples (10 induced sputa, 12 transthoracic needle aspirations and 2 bronchoalveolar lavages) from 17 patients with AIDS and respiratory symptoms were studied. We performed DNA amplification by using 2 primers that amplify the mitochondrial large rDNA sequence of P. carinii. RESULTS: The sensitivity and specificity of the PCR technique were 92.8% and 100% respectively, with a positive predictive value of 100% and negative predictive value of 92.3%. The sensitivity of the silver stain were 65%, specificity 100%, positive predictive value 100% and negative predictive value 63%. CONCLUSION: The PCR technique is more sensitive than the methenamine silver stain for detecting Pneumocystis carinii in respiratory specimens from patients with pneumonia. Consequently it could be a very good alternative to classical methods in diagnosis of P. carinii infections. SN - 0213-005X UR - https://www.unboundmedicine.com/medline/citation/7684613/[Detection_of_Pneumocystis_carinii_in_clinical_samples_by_the_polymerase_chain_reaction__Comparison_with_the_methenamine_silver_technique]_ L2 - https://medlineplus.gov/hivaidsandinfections.html DB - PRIME DP - Unbound Medicine ER -