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Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells.
Exp Cell Res. 1993 Jun; 206(2):311-7.EC

Abstract

The present study was designed to reexamine the interaction of granulocyte-macrophage colony-stimulating factor (GM-CSF) with endothelial cells (EC) and to investigate the expression of CSF receptor chains in these cells. In agreement with previous data, GM-CSF induced directional migration and, to a lesser degree, proliferation of human umbilical vein EC. When compared to basic fibroblast growth factor, GM-CSF was comparable in terms of chemotactic activity and was substantially less active in terms of proliferation. Binding studies confirmed the presence of receptors for GM-CSF (GM-CSFR) on EC. The expression of the beta chain common to the GM-CSFR, IL-3 receptor, and IL-5 receptor, as well as of the individual alpha chains, was studied by Northern analysis and/or reverse transcription and polymerase chain reaction. EC expressed high levels of the common beta chain transcripts. Expression of the alpha(GM) and alpha(IL-5) chain mRNA was minimal or absent in normal EC, though the transformed ECV304 endothelial cell line had substantial amounts of alpha(GM) chain mRNA. Unexpectedly, EC expressed alpha(IL-3) chain transcripts. IL-3 induced migration of EC across polycarbonate filters, whereas IL-5 was inactive.

Authors+Show Affiliations

Colotta F
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Bussolino F
No affiliation info available
Polentarutti N
No affiliation info available
Guglielmetti A
No affiliation info available
Sironi M
No affiliation info available
Bocchietto E
No affiliation info available
De Rossi M
No affiliation info available
Mantovani A
No affiliation info available

MeSH

AnimalsBase SequenceCell DivisionCells, CulturedChemotaxisEndothelium, VascularGranulocyte-Macrophage Colony-Stimulating FactorHumansInterleukin-3Interleukin-5KineticsMacromolecular SubstancesMiceMolecular Sequence DataOligodeoxyribonucleotidesPolymerase Chain ReactionRNARNA, MessengerReceptors, Granulocyte-Macrophage Colony-Stimulating FactorReceptors, ImmunologicReceptors, InterleukinReceptors, Interleukin-3Receptors, Interleukin-5Recombinant ProteinsTranscription, GeneticUmbilical Veins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7684696

Citation

Colotta, F, et al. "Differential Expression of the Common Beta and Specific Alpha Chains of the Receptors for GM-CSF, IL-3, and IL-5 in Endothelial Cells." Experimental Cell Research, vol. 206, no. 2, 1993, pp. 311-7.
Colotta F, Bussolino F, Polentarutti N, et al. Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. Exp Cell Res. 1993;206(2):311-7.
Colotta, F., Bussolino, F., Polentarutti, N., Guglielmetti, A., Sironi, M., Bocchietto, E., De Rossi, M., & Mantovani, A. (1993). Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. Experimental Cell Research, 206(2), 311-7.
Colotta F, et al. Differential Expression of the Common Beta and Specific Alpha Chains of the Receptors for GM-CSF, IL-3, and IL-5 in Endothelial Cells. Exp Cell Res. 1993;206(2):311-7. PubMed PMID: 7684696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. AU - Colotta,F, AU - Bussolino,F, AU - Polentarutti,N, AU - Guglielmetti,A, AU - Sironi,M, AU - Bocchietto,E, AU - De Rossi,M, AU - Mantovani,A, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 311 EP - 7 JF - Experimental cell research JO - Exp Cell Res VL - 206 IS - 2 N2 - The present study was designed to reexamine the interaction of granulocyte-macrophage colony-stimulating factor (GM-CSF) with endothelial cells (EC) and to investigate the expression of CSF receptor chains in these cells. In agreement with previous data, GM-CSF induced directional migration and, to a lesser degree, proliferation of human umbilical vein EC. When compared to basic fibroblast growth factor, GM-CSF was comparable in terms of chemotactic activity and was substantially less active in terms of proliferation. Binding studies confirmed the presence of receptors for GM-CSF (GM-CSFR) on EC. The expression of the beta chain common to the GM-CSFR, IL-3 receptor, and IL-5 receptor, as well as of the individual alpha chains, was studied by Northern analysis and/or reverse transcription and polymerase chain reaction. EC expressed high levels of the common beta chain transcripts. Expression of the alpha(GM) and alpha(IL-5) chain mRNA was minimal or absent in normal EC, though the transformed ECV304 endothelial cell line had substantial amounts of alpha(GM) chain mRNA. Unexpectedly, EC expressed alpha(IL-3) chain transcripts. IL-3 induced migration of EC across polycarbonate filters, whereas IL-5 was inactive. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/7684696/Differential_expression_of_the_common_beta_and_specific_alpha_chains_of_the_receptors_for_GM_CSF_IL_3_and_IL_5_in_endothelial_cells_ DB - PRIME DP - Unbound Medicine ER -
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