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Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells.
Exp Cell Res. 1993 Jun; 206(2):311-7.EC

Abstract

The present study was designed to reexamine the interaction of granulocyte-macrophage colony-stimulating factor (GM-CSF) with endothelial cells (EC) and to investigate the expression of CSF receptor chains in these cells. In agreement with previous data, GM-CSF induced directional migration and, to a lesser degree, proliferation of human umbilical vein EC. When compared to basic fibroblast growth factor, GM-CSF was comparable in terms of chemotactic activity and was substantially less active in terms of proliferation. Binding studies confirmed the presence of receptors for GM-CSF (GM-CSFR) on EC. The expression of the beta chain common to the GM-CSFR, IL-3 receptor, and IL-5 receptor, as well as of the individual alpha chains, was studied by Northern analysis and/or reverse transcription and polymerase chain reaction. EC expressed high levels of the common beta chain transcripts. Expression of the alpha(GM) and alpha(IL-5) chain mRNA was minimal or absent in normal EC, though the transformed ECV304 endothelial cell line had substantial amounts of alpha(GM) chain mRNA. Unexpectedly, EC expressed alpha(IL-3) chain transcripts. IL-3 induced migration of EC across polycarbonate filters, whereas IL-5 was inactive.

Authors+Show Affiliations

Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7684696

Citation

Colotta, F, et al. "Differential Expression of the Common Beta and Specific Alpha Chains of the Receptors for GM-CSF, IL-3, and IL-5 in Endothelial Cells." Experimental Cell Research, vol. 206, no. 2, 1993, pp. 311-7.
Colotta F, Bussolino F, Polentarutti N, et al. Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. Exp Cell Res. 1993;206(2):311-7.
Colotta, F., Bussolino, F., Polentarutti, N., Guglielmetti, A., Sironi, M., Bocchietto, E., De Rossi, M., & Mantovani, A. (1993). Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. Experimental Cell Research, 206(2), 311-7.
Colotta F, et al. Differential Expression of the Common Beta and Specific Alpha Chains of the Receptors for GM-CSF, IL-3, and IL-5 in Endothelial Cells. Exp Cell Res. 1993;206(2):311-7. PubMed PMID: 7684696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of the common beta and specific alpha chains of the receptors for GM-CSF, IL-3, and IL-5 in endothelial cells. AU - Colotta,F, AU - Bussolino,F, AU - Polentarutti,N, AU - Guglielmetti,A, AU - Sironi,M, AU - Bocchietto,E, AU - De Rossi,M, AU - Mantovani,A, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 311 EP - 7 JF - Experimental cell research JO - Exp Cell Res VL - 206 IS - 2 N2 - The present study was designed to reexamine the interaction of granulocyte-macrophage colony-stimulating factor (GM-CSF) with endothelial cells (EC) and to investigate the expression of CSF receptor chains in these cells. In agreement with previous data, GM-CSF induced directional migration and, to a lesser degree, proliferation of human umbilical vein EC. When compared to basic fibroblast growth factor, GM-CSF was comparable in terms of chemotactic activity and was substantially less active in terms of proliferation. Binding studies confirmed the presence of receptors for GM-CSF (GM-CSFR) on EC. The expression of the beta chain common to the GM-CSFR, IL-3 receptor, and IL-5 receptor, as well as of the individual alpha chains, was studied by Northern analysis and/or reverse transcription and polymerase chain reaction. EC expressed high levels of the common beta chain transcripts. Expression of the alpha(GM) and alpha(IL-5) chain mRNA was minimal or absent in normal EC, though the transformed ECV304 endothelial cell line had substantial amounts of alpha(GM) chain mRNA. Unexpectedly, EC expressed alpha(IL-3) chain transcripts. IL-3 induced migration of EC across polycarbonate filters, whereas IL-5 was inactive. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/7684696/Differential_expression_of_the_common_beta_and_specific_alpha_chains_of_the_receptors_for_GM_CSF_IL_3_and_IL_5_in_endothelial_cells_ DB - PRIME DP - Unbound Medicine ER -