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Interferon-gamma enhances the LPS-induced G-CSF gene expression in human adherent monocytes, which is regulated at transcriptional and posttranscriptional levels.
Exp Hematol. 1993 Jun; 21(6):785-90.EH

Abstract

Human adherent monocytes were studied with regard to the expression of granulocyte colony-stimulating factor (G-CSF) at mRNA and protein levels in response to lipopolysaccharide (LPS) and gamma-interferon (IFN-gamma) stimulation. Monocytes did not express G-CSF transcripts in response to IFN-gamma treatment. In contrast, monocytes exposed to IFN-gamma plus LPS showed a dose-dependent increase in G-CSF mRNA accumulation and protein secretion compared to LPS-stimulated monocytes. The augmented G-CSF mRNA expression in response to IFN-gamma plus LPS was the result of a slight increase in the G-CSF transcription rate (2.2-fold) and a more than 6-fold increase in the G-CSF mRNA half-life (20 minutes vs. > 120 minutes). In addition, it was shown that the effects of IFN-gamma on LPS-induced G-CSF protein secretion could be mimicked by the calcium ionophore A23187, suggesting that the Ca(2+)-dependent pathway might be triggered after binding of the ligand to the receptor. Finally, it was observed that the potentiating effects of IFN-gamma on LPS-induced G-CSF secretion could be blocked by interleukin-4 (IL-4). These data indicate that two cytokines produced by activated T cells have opposite effects on G-CSF production by human activated monocytes.

Authors+Show Affiliations

Department of Medicine, University of Groningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7684703

Citation

de Wit, H, et al. "Interferon-gamma Enhances the LPS-induced G-CSF Gene Expression in Human Adherent Monocytes, Which Is Regulated at Transcriptional and Posttranscriptional Levels." Experimental Hematology, vol. 21, no. 6, 1993, pp. 785-90.
de Wit H, Dokter WH, Esselink MT, et al. Interferon-gamma enhances the LPS-induced G-CSF gene expression in human adherent monocytes, which is regulated at transcriptional and posttranscriptional levels. Exp Hematol. 1993;21(6):785-90.
de Wit, H., Dokter, W. H., Esselink, M. T., Halie, M. R., & Vellenga, E. (1993). Interferon-gamma enhances the LPS-induced G-CSF gene expression in human adherent monocytes, which is regulated at transcriptional and posttranscriptional levels. Experimental Hematology, 21(6), 785-90.
de Wit H, et al. Interferon-gamma Enhances the LPS-induced G-CSF Gene Expression in Human Adherent Monocytes, Which Is Regulated at Transcriptional and Posttranscriptional Levels. Exp Hematol. 1993;21(6):785-90. PubMed PMID: 7684703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interferon-gamma enhances the LPS-induced G-CSF gene expression in human adherent monocytes, which is regulated at transcriptional and posttranscriptional levels. AU - de Wit,H, AU - Dokter,W H, AU - Esselink,M T, AU - Halie,M R, AU - Vellenga,E, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 785 EP - 90 JF - Experimental hematology JO - Exp Hematol VL - 21 IS - 6 N2 - Human adherent monocytes were studied with regard to the expression of granulocyte colony-stimulating factor (G-CSF) at mRNA and protein levels in response to lipopolysaccharide (LPS) and gamma-interferon (IFN-gamma) stimulation. Monocytes did not express G-CSF transcripts in response to IFN-gamma treatment. In contrast, monocytes exposed to IFN-gamma plus LPS showed a dose-dependent increase in G-CSF mRNA accumulation and protein secretion compared to LPS-stimulated monocytes. The augmented G-CSF mRNA expression in response to IFN-gamma plus LPS was the result of a slight increase in the G-CSF transcription rate (2.2-fold) and a more than 6-fold increase in the G-CSF mRNA half-life (20 minutes vs. > 120 minutes). In addition, it was shown that the effects of IFN-gamma on LPS-induced G-CSF protein secretion could be mimicked by the calcium ionophore A23187, suggesting that the Ca(2+)-dependent pathway might be triggered after binding of the ligand to the receptor. Finally, it was observed that the potentiating effects of IFN-gamma on LPS-induced G-CSF secretion could be blocked by interleukin-4 (IL-4). These data indicate that two cytokines produced by activated T cells have opposite effects on G-CSF production by human activated monocytes. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/7684703/Interferon_gamma_enhances_the_LPS_induced_G_CSF_gene_expression_in_human_adherent_monocytes_which_is_regulated_at_transcriptional_and_posttranscriptional_levels_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:7684703 DB - PRIME DP - Unbound Medicine ER -