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The expression of major histocompatibility antigens on human articular chondrocytes.

Abstract

Human articular chondrocytes were analyzed for major histocompatibility (MHC) antigens. The cells were obtained by enzymatic digestion of surgical specimens from patients with osteoarthrosis and rheumatoid arthritis having joint replacement. The analyses of Class I and Class II antigens were performed using microcytotoxicity techniques, as with conventional human leukocyte antigen (HLA) typing, and by immunofluorescence on a flow cytometer with monoclonal antibodies. Eighty-five percent of the chondrocytes had strong representation of Class I molecules, and individual specificities for HLA-A, HLA-B, and HLA-C could be detected. Class II antigens generally were not expressed on chondrocytes from noninflammatory states. In osteoarthrosis, some cells expressed HLA-DP and HLA-DQ, whereas in rheumatoid arthritis, there was an increase in the expression of all Class II antigens. Incubation of chondrocytes with gamma-interferon caused the strong induction of HLA-DR and HLA-DP, whereas HLA-DQ expression was largely unaffected. These results have broad implications for tissue transplantation and autoimmune disease.

Authors+Show Affiliations

Shriners Hospitals for Crippled Children, Honolulu Unit, Hawaii 96826.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7684959

Citation

Lance, E M., et al. "The Expression of Major Histocompatibility Antigens On Human Articular Chondrocytes." Clinical Orthopaedics and Related Research, 1993, pp. 266-82.
Lance EM, Kimura LH, Manibog CN. The expression of major histocompatibility antigens on human articular chondrocytes. Clin Orthop Relat Res. 1993.
Lance, E. M., Kimura, L. H., & Manibog, C. N. (1993). The expression of major histocompatibility antigens on human articular chondrocytes. Clinical Orthopaedics and Related Research, (291), 266-82.
Lance EM, Kimura LH, Manibog CN. The Expression of Major Histocompatibility Antigens On Human Articular Chondrocytes. Clin Orthop Relat Res. 1993;(291)266-82. PubMed PMID: 7684959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The expression of major histocompatibility antigens on human articular chondrocytes. AU - Lance,E M, AU - Kimura,L H, AU - Manibog,C N, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 266 EP - 82 JF - Clinical orthopaedics and related research JO - Clin Orthop Relat Res IS - 291 N2 - Human articular chondrocytes were analyzed for major histocompatibility (MHC) antigens. The cells were obtained by enzymatic digestion of surgical specimens from patients with osteoarthrosis and rheumatoid arthritis having joint replacement. The analyses of Class I and Class II antigens were performed using microcytotoxicity techniques, as with conventional human leukocyte antigen (HLA) typing, and by immunofluorescence on a flow cytometer with monoclonal antibodies. Eighty-five percent of the chondrocytes had strong representation of Class I molecules, and individual specificities for HLA-A, HLA-B, and HLA-C could be detected. Class II antigens generally were not expressed on chondrocytes from noninflammatory states. In osteoarthrosis, some cells expressed HLA-DP and HLA-DQ, whereas in rheumatoid arthritis, there was an increase in the expression of all Class II antigens. Incubation of chondrocytes with gamma-interferon caused the strong induction of HLA-DR and HLA-DP, whereas HLA-DQ expression was largely unaffected. These results have broad implications for tissue transplantation and autoimmune disease. SN - 0009-921X UR - https://www.unboundmedicine.com/medline/citation/7684959/The_expression_of_major_histocompatibility_antigens_on_human_articular_chondrocytes_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=7684959.ui DB - PRIME DP - Unbound Medicine ER -