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A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies.
Biotechniques. 1993 Jun; 14(6):925-30.B

Abstract

Proteins stained with Coomassie Brilliant Blue I were transferred effectively by blotting from polyacrylamide gel electrophoresis (PAGE) gels to transparencies of the type used in plain-paper copiers. The details of the original electropherogram were retained on transfer and did not fade over a period of three years. Both the protein and the associated dye transfer; however, protein does not transfer in the absence of dye. Protein patterns present in several types of gels--sodium dodecyl sulfate (SDS)-PAGE, non-denaturing PAGE, isoelectric focusing PAGE and SDS-agarose--all transferred successfully after staining with dye 1. Proteins visualized with other organic dyes such as Fast Green FCF 2, Uniblue A 3 and Procion Blue MX-R 4 also transferred, but proteins stained with Stains-all 5 or silver did not. This transfer provides a simple, economical way to preserve data from slab gel electrophoresis and a convenient method to display data using an overhead projector. The blotted transparencies are also excellent substrates for use with gel scanning densitometers.

Authors+Show Affiliations

Department of Chemistry, Harvard University, Cambridge, MA 02138.No affiliation info available

Pub Type(s)

Research Support, U.S. Gov't, Non-P.H.S.
Technical Report

Language

eng

PubMed ID

7687447

Citation

Chu, Y H., and G M. Whitesides. "A Convenient Procedure for Transfer Blotting of Coomassie Blue Stained Proteins From PAGE Gels to Transparencies." BioTechniques, vol. 14, no. 6, 1993, pp. 925-30.
Chu YH, Whitesides GM. A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies. Biotechniques. 1993;14(6):925-30.
Chu, Y. H., & Whitesides, G. M. (1993). A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies. BioTechniques, 14(6), 925-30.
Chu YH, Whitesides GM. A Convenient Procedure for Transfer Blotting of Coomassie Blue Stained Proteins From PAGE Gels to Transparencies. Biotechniques. 1993;14(6):925-30. PubMed PMID: 7687447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies. AU - Chu,Y H, AU - Whitesides,G M, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 925 EP - 30 JF - BioTechniques JO - Biotechniques VL - 14 IS - 6 N2 - Proteins stained with Coomassie Brilliant Blue I were transferred effectively by blotting from polyacrylamide gel electrophoresis (PAGE) gels to transparencies of the type used in plain-paper copiers. The details of the original electropherogram were retained on transfer and did not fade over a period of three years. Both the protein and the associated dye transfer; however, protein does not transfer in the absence of dye. Protein patterns present in several types of gels--sodium dodecyl sulfate (SDS)-PAGE, non-denaturing PAGE, isoelectric focusing PAGE and SDS-agarose--all transferred successfully after staining with dye 1. Proteins visualized with other organic dyes such as Fast Green FCF 2, Uniblue A 3 and Procion Blue MX-R 4 also transferred, but proteins stained with Stains-all 5 or silver did not. This transfer provides a simple, economical way to preserve data from slab gel electrophoresis and a convenient method to display data using an overhead projector. The blotted transparencies are also excellent substrates for use with gel scanning densitometers. SN - 0736-6205 UR - https://www.unboundmedicine.com/medline/citation/7687447/A_convenient_procedure_for_transfer_blotting_of_coomassie_blue_stained_proteins_from_PAGE_gels_to_transparencies_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:7687447 DB - PRIME DP - Unbound Medicine ER -