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Tyrosine phosphorylation of CD19 in pre-B and mature B cells.
EMBO J. 1993 Jul; 12(7):2691-6.EJ

Abstract

Cross-linking of B cell surface immunoglobulins (sIg) results in activation of mature B cells and stimulates a molecular signaling mechanism for antigen-specific B cell expansion and differentiation. This signaling pathway is dependent on tyrosine (Tyr) phosphorylation and results in the activation of sIg-associated src family kinases and p72SYK. Rapid Tyr phosphorylation occurs on multiple protein substrates. Here we show that activation of B cells by cross-linking sIg results in an increase in Tyr phosphorylation of the lineage-restricted B cell surface antigen CD19, and show that it is a major substrate of activated Tyr kinase following sIg stimulation. Lower levels of constitutive CD19 Tyr phosphorylation occurred in most sIg+ mature B cell lines examined and in normal dense tonsillar B cells. We also find that when CD19 is Tyr-phosphorylated it becomes competent to interact with SH2 domains suggesting a mechanism whereby, following B cell activation, CD19 could be linked to intracellular signaling pathways. In sIg- pre-B cell lines, CD19 was expressed but was not constitutively phosphorylated on tyrosine. Upon CD19 cross-linking, Tyr phosphorylation of CD19 was induced in sIg- pre-B cell lines. CD19 cross-linking also directly induced Tyr phosphorylation of CD19 and other substrates in mature B cells. The ability of CD19 to signal in the absence of sIg expression may provide important stimulation in pre-B cell development.

Authors+Show Affiliations

Bristol-Myers Squibb, Pharmaceutical Research Institute, Seattle, WA 98121.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7687539

Citation

Chalupny, N J., et al. "Tyrosine Phosphorylation of CD19 in pre-B and Mature B Cells." The EMBO Journal, vol. 12, no. 7, 1993, pp. 2691-6.
Chalupny NJ, Kanner SB, Schieven GL, et al. Tyrosine phosphorylation of CD19 in pre-B and mature B cells. EMBO J. 1993;12(7):2691-6.
Chalupny, N. J., Kanner, S. B., Schieven, G. L., Wee, S. F., Gilliland, L. K., Aruffo, A., & Ledbetter, J. A. (1993). Tyrosine phosphorylation of CD19 in pre-B and mature B cells. The EMBO Journal, 12(7), 2691-6.
Chalupny NJ, et al. Tyrosine Phosphorylation of CD19 in pre-B and Mature B Cells. EMBO J. 1993;12(7):2691-6. PubMed PMID: 7687539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tyrosine phosphorylation of CD19 in pre-B and mature B cells. AU - Chalupny,N J, AU - Kanner,S B, AU - Schieven,G L, AU - Wee,S F, AU - Gilliland,L K, AU - Aruffo,A, AU - Ledbetter,J A, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 2691 EP - 6 JF - The EMBO journal JO - EMBO J VL - 12 IS - 7 N2 - Cross-linking of B cell surface immunoglobulins (sIg) results in activation of mature B cells and stimulates a molecular signaling mechanism for antigen-specific B cell expansion and differentiation. This signaling pathway is dependent on tyrosine (Tyr) phosphorylation and results in the activation of sIg-associated src family kinases and p72SYK. Rapid Tyr phosphorylation occurs on multiple protein substrates. Here we show that activation of B cells by cross-linking sIg results in an increase in Tyr phosphorylation of the lineage-restricted B cell surface antigen CD19, and show that it is a major substrate of activated Tyr kinase following sIg stimulation. Lower levels of constitutive CD19 Tyr phosphorylation occurred in most sIg+ mature B cell lines examined and in normal dense tonsillar B cells. We also find that when CD19 is Tyr-phosphorylated it becomes competent to interact with SH2 domains suggesting a mechanism whereby, following B cell activation, CD19 could be linked to intracellular signaling pathways. In sIg- pre-B cell lines, CD19 was expressed but was not constitutively phosphorylated on tyrosine. Upon CD19 cross-linking, Tyr phosphorylation of CD19 was induced in sIg- pre-B cell lines. CD19 cross-linking also directly induced Tyr phosphorylation of CD19 and other substrates in mature B cells. The ability of CD19 to signal in the absence of sIg expression may provide important stimulation in pre-B cell development. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/7687539/Tyrosine_phosphorylation_of_CD19_in_pre_B_and_mature_B_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1993&volume=12&issue=7&spage=2691 DB - PRIME DP - Unbound Medicine ER -