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Apoptosis of human erythroid colony-forming cells is decreased by stem cell factor and insulin-like growth factor I as well as erythropoietin.
J Cell Physiol. 1993 Aug; 156(2):264-71.JC

Abstract

To clarify the manner by which erythropoietin (EP), stem cell factor (SCF), or insulin-like growth factor I (IGF-I) regulate erythropoiesis, apoptosis of human erythroid progenitor cells was investigated. Human burst-forming units-erythroid (BFU-E) partially purified from peripheral blood were cultured for 6 days to generate erythroid colony-forming cells (ECFC), which consist mainly of colony-forming units-erythroid (CFU-E). The cells were labeled with [3H]thymidine, incubated in serum-free liquid media, at 37 degrees C, for 16 h, and the pattern of DNA breakdown was analyzed by agarose gel electrophoresis. When these cells were incubated without EP, 70% of the total cellular DNA was broken down into DNA fragments of less than 5 kilobases and nuclear condensation and fragmentation, characteristic of apoptosis, were evident. While EP greatly reduced the amount of DNA breakdown to 23%, SCF and IGF-I each reduced the amount of DNA breakdown to 38-46% and, when added together, to 24%. Dose-response experiments with SCF and IGF-I showed a dose-dependent reduction in DNA fragmentation at concentrations that stimulate colony formation in serum-free semisolid cultures. Finally, assays of ECFC performed by the plasma clot method, after serum-free liquid culture, at 37 degrees C, for 16 h, demonstrated marked protection of erythroid colony-forming capacity by SCF or IGF-I in the absence of EP, as well as by EP itself. These data indicate that human erythroid progenitor cells undergo apoptosis which is reduced by SCF and IGF-I as well as EP and suggest that the control of apoptosis by each of these factors has a prominent role in the regulation of erythropoiesis.

Authors+Show Affiliations

Department of Medicine, Nashville Department of Veterans Affairs Medical Center, Tennessee.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7688370

Citation

Muta, K, and S B. Krantz. "Apoptosis of Human Erythroid Colony-forming Cells Is Decreased By Stem Cell Factor and Insulin-like Growth Factor I as Well as Erythropoietin." Journal of Cellular Physiology, vol. 156, no. 2, 1993, pp. 264-71.
Muta K, Krantz SB. Apoptosis of human erythroid colony-forming cells is decreased by stem cell factor and insulin-like growth factor I as well as erythropoietin. J Cell Physiol. 1993;156(2):264-71.
Muta, K., & Krantz, S. B. (1993). Apoptosis of human erythroid colony-forming cells is decreased by stem cell factor and insulin-like growth factor I as well as erythropoietin. Journal of Cellular Physiology, 156(2), 264-71.
Muta K, Krantz SB. Apoptosis of Human Erythroid Colony-forming Cells Is Decreased By Stem Cell Factor and Insulin-like Growth Factor I as Well as Erythropoietin. J Cell Physiol. 1993;156(2):264-71. PubMed PMID: 7688370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis of human erythroid colony-forming cells is decreased by stem cell factor and insulin-like growth factor I as well as erythropoietin. AU - Muta,K, AU - Krantz,S B, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 264 EP - 71 JF - Journal of cellular physiology JO - J Cell Physiol VL - 156 IS - 2 N2 - To clarify the manner by which erythropoietin (EP), stem cell factor (SCF), or insulin-like growth factor I (IGF-I) regulate erythropoiesis, apoptosis of human erythroid progenitor cells was investigated. Human burst-forming units-erythroid (BFU-E) partially purified from peripheral blood were cultured for 6 days to generate erythroid colony-forming cells (ECFC), which consist mainly of colony-forming units-erythroid (CFU-E). The cells were labeled with [3H]thymidine, incubated in serum-free liquid media, at 37 degrees C, for 16 h, and the pattern of DNA breakdown was analyzed by agarose gel electrophoresis. When these cells were incubated without EP, 70% of the total cellular DNA was broken down into DNA fragments of less than 5 kilobases and nuclear condensation and fragmentation, characteristic of apoptosis, were evident. While EP greatly reduced the amount of DNA breakdown to 23%, SCF and IGF-I each reduced the amount of DNA breakdown to 38-46% and, when added together, to 24%. Dose-response experiments with SCF and IGF-I showed a dose-dependent reduction in DNA fragmentation at concentrations that stimulate colony formation in serum-free semisolid cultures. Finally, assays of ECFC performed by the plasma clot method, after serum-free liquid culture, at 37 degrees C, for 16 h, demonstrated marked protection of erythroid colony-forming capacity by SCF or IGF-I in the absence of EP, as well as by EP itself. These data indicate that human erythroid progenitor cells undergo apoptosis which is reduced by SCF and IGF-I as well as EP and suggest that the control of apoptosis by each of these factors has a prominent role in the regulation of erythropoiesis. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/7688370/Apoptosis_of_human_erythroid_colony_forming_cells_is_decreased_by_stem_cell_factor_and_insulin_like_growth_factor_I_as_well_as_erythropoietin_ L2 - https://doi.org/10.1002/jcp.1041560207 DB - PRIME DP - Unbound Medicine ER -