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Efficacy and limitations of QBC acridine orange staining as a routine diagnostic technique for malaria in developing countries.
J Trop Med Hyg. 1993 Aug; 96(4):245-8.JT

Abstract

The recently developed QBC technique has been reported to be rapid, sensitive and specific for the detection of malaria infection. We evaluated this technique in comparison with thin and thick Giemsa stained blood films for the diagnosis of malaria in acute fever and PUO cases. The technique was slightly more sensitive than thin blood film but equal to thick blood film examination. Identification of species of malaria posed some difficulties. Due to its high cost and requirement for a fluorescent microscope this technique, in spite of its speed and sensitivity, may not have the potential to be a substitute for the conventional blood film examination for the diagnosis of malaria, especially in developing countries.

Authors+Show Affiliations

Kumar BK
Department of Haematology, Sana'a Medical School, Republic of Yemen.
al Fadeel M
No affiliation info available
Sehgal SC
No affiliation info available

MeSH

Acridine OrangeAnimalsAzure StainsDeveloping CountriesEvaluation Studies as TopicHumansMalaria, FalciparumMalaria, VivaxMicroscopy, FluorescencePlasmodium falciparumPlasmodium vivaxReagent Kits, DiagnosticSensitivity and SpecificityStaining and LabelingYemen

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7688429

Citation

Kumar, B K., et al. "Efficacy and Limitations of QBC Acridine Orange Staining as a Routine Diagnostic Technique for Malaria in Developing Countries." The Journal of Tropical Medicine and Hygiene, vol. 96, no. 4, 1993, pp. 245-8.
Kumar BK, al Fadeel M, Sehgal SC. Efficacy and limitations of QBC acridine orange staining as a routine diagnostic technique for malaria in developing countries. J Trop Med Hyg. 1993;96(4):245-8.
Kumar, B. K., al Fadeel, M., & Sehgal, S. C. (1993). Efficacy and limitations of QBC acridine orange staining as a routine diagnostic technique for malaria in developing countries. The Journal of Tropical Medicine and Hygiene, 96(4), 245-8.
Kumar BK, al Fadeel M, Sehgal SC. Efficacy and Limitations of QBC Acridine Orange Staining as a Routine Diagnostic Technique for Malaria in Developing Countries. J Trop Med Hyg. 1993;96(4):245-8. PubMed PMID: 7688429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy and limitations of QBC acridine orange staining as a routine diagnostic technique for malaria in developing countries. AU - Kumar,B K, AU - al Fadeel,M, AU - Sehgal,S C, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 245 EP - 8 JF - The Journal of tropical medicine and hygiene JO - J Trop Med Hyg VL - 96 IS - 4 N2 - The recently developed QBC technique has been reported to be rapid, sensitive and specific for the detection of malaria infection. We evaluated this technique in comparison with thin and thick Giemsa stained blood films for the diagnosis of malaria in acute fever and PUO cases. The technique was slightly more sensitive than thin blood film but equal to thick blood film examination. Identification of species of malaria posed some difficulties. Due to its high cost and requirement for a fluorescent microscope this technique, in spite of its speed and sensitivity, may not have the potential to be a substitute for the conventional blood film examination for the diagnosis of malaria, especially in developing countries. SN - 0022-5304 UR - https://www.unboundmedicine.com/medline/citation/7688429/Efficacy_and_limitations_of_QBC_acridine_orange_staining_as_a_routine_diagnostic_technique_for_malaria_in_developing_countries_ L2 - http://www.diseaseinfosearch.org/result/4415 DB - PRIME DP - Unbound Medicine ER -