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A monoclonal antibody Fab fragment crystallized with and without a peptide epitope from HIV.
Proteins. 1993 Aug; 16(4):437-9.P

Abstract

The Fab fragment of CB 4-1, a monoclonal murine antibody against HIV protein p24, has been produced. It forms a complex with a synthetic antigen, an epitope of p24 made up of 11 amino acids, with the binding constant Kd = 3.6 x 10(-9) M. Crystals of hexagonal and orthorhombic space group has been obtained by cocrystallization of the Fab with the epitope and crystallization without the epitope, respectively. In either case, the crystals are suitable for X-ray structural analysis. Crystals of the Fab fragment cocrystallized with the peptide have the space group P 6(3)22 with cell dimensions of a = b = 105 A, c = 297 A. Fab crystals without the epitope are in space group C 222 with cell dimensions a = 110.1 A, b = 110.2 A, c = 150.1 A.

Authors+Show Affiliations

Pfeffer-Hennig S
Institute of Biochemistry, Humboldt University, Berlin, Germany.
Wessner H
No affiliation info available
Hennig M
No affiliation info available
Dauter Z
No affiliation info available
Hausdorf G
No affiliation info available
Höhne WE
No affiliation info available

MeSH

AnimalsAntibodies, MonoclonalAntigen-Antibody ComplexCrystallizationEpitopesHIVHIV AntibodiesHIV Core Protein p24Immunoglobulin Fab FragmentsMiceX-Ray Diffraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7689218

Citation

Pfeffer-Hennig, S, et al. "A Monoclonal Antibody Fab Fragment Crystallized With and Without a Peptide Epitope From HIV." Proteins, vol. 16, no. 4, 1993, pp. 437-9.
Pfeffer-Hennig S, Wessner H, Hennig M, et al. A monoclonal antibody Fab fragment crystallized with and without a peptide epitope from HIV. Proteins. 1993;16(4):437-9.
Pfeffer-Hennig, S., Wessner, H., Hennig, M., Dauter, Z., Hausdorf, G., & Höhne, W. E. (1993). A monoclonal antibody Fab fragment crystallized with and without a peptide epitope from HIV. Proteins, 16(4), 437-9.
Pfeffer-Hennig S, et al. A Monoclonal Antibody Fab Fragment Crystallized With and Without a Peptide Epitope From HIV. Proteins. 1993;16(4):437-9. PubMed PMID: 7689218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A monoclonal antibody Fab fragment crystallized with and without a peptide epitope from HIV. AU - Pfeffer-Hennig,S, AU - Wessner,H, AU - Hennig,M, AU - Dauter,Z, AU - Hausdorf,G, AU - Höhne,W E, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 437 EP - 9 JF - Proteins JO - Proteins VL - 16 IS - 4 N2 - The Fab fragment of CB 4-1, a monoclonal murine antibody against HIV protein p24, has been produced. It forms a complex with a synthetic antigen, an epitope of p24 made up of 11 amino acids, with the binding constant Kd = 3.6 x 10(-9) M. Crystals of hexagonal and orthorhombic space group has been obtained by cocrystallization of the Fab with the epitope and crystallization without the epitope, respectively. In either case, the crystals are suitable for X-ray structural analysis. Crystals of the Fab fragment cocrystallized with the peptide have the space group P 6(3)22 with cell dimensions of a = b = 105 A, c = 297 A. Fab crystals without the epitope are in space group C 222 with cell dimensions a = 110.1 A, b = 110.2 A, c = 150.1 A. SN - 0887-3585 UR - https://www.unboundmedicine.com/medline/citation/7689218/A_monoclonal_antibody_Fab_fragment_crystallized_with_and_without_a_peptide_epitope_from_HIV_ L2 - https://doi.org/10.1002/prot.340160411 DB - PRIME DP - Unbound Medicine ER -