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Phenotypic characterization of the human fibrous histiocytoma giant cell tumor (GCT) cell line and its cytokine repertoire.
Exp Hematol. 1993 Sep; 21(10):1342-52.EH

Abstract

The pleiotropic nature of malignant fibrous histiocytomas (MFH) is manifested as mixed cellular infiltrates consisting of myofibroblasts, histiomonocytes, and neutrophils. We detail in this report the phenotypic characteristics of the human fibrous histiocytoma giant cell tumor (GCT) cell line that establish its mesenchymal origin. The latter is underscored by the ability of GCT cells to express mRNA for transforming growth factor beta (TGF-beta) as well as both A and B chains of platelet-derived growth factor (PDGF). GCT cells also support the binding of CD34+ cells, but less efficiently than do normal marrow stromal cells. Since cytokines elaborated by MFH may mediate in part the recruitment of monocytes and neutrophils into tumor-infiltrated tissues, we have determined the cytokine repertoire of the GCT cell line, already known for its ability to elaborate colony-stimulating factors (CSFs) and interleukin-1 (IL-1). GCT cells express IL-1 alpha, IL-1 beta, IL-6, macrophage colony-stimulating factor (M-CSF or CSF-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and IL-8. No detectable mRNA for IL-3, IL-4, IL-7, and tumor necrosis factor-alpha (TNF-alpha) was detected in GCT cells by polymerase chain reaction (PCR). Expression of cytokine mRNAs was responsive to agents such as dexamethasone (dex), 12-O-tetradecanoyl phorbol 13-acetate (phorbol diester or TPA), and TNF-alpha. Thus, this cell line provides a useful model for understanding the pathobiology of MFH and hematopoietic progenitor interactions with mesenchymal/stromal cells.

Authors+Show Affiliations

Department of Medicine, University of Rochester School of Medicine, NY 14642.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7689482

Citation

Liesveld, J L., et al. "Phenotypic Characterization of the Human Fibrous Histiocytoma Giant Cell Tumor (GCT) Cell Line and Its Cytokine Repertoire." Experimental Hematology, vol. 21, no. 10, 1993, pp. 1342-52.
Liesveld JL, Rush S, Kempski MC, et al. Phenotypic characterization of the human fibrous histiocytoma giant cell tumor (GCT) cell line and its cytokine repertoire. Exp Hematol. 1993;21(10):1342-52.
Liesveld, J. L., Rush, S., Kempski, M. C., Turner, A. R., Brennan, J. K., Gasson, J. C., & Abboud, C. N. (1993). Phenotypic characterization of the human fibrous histiocytoma giant cell tumor (GCT) cell line and its cytokine repertoire. Experimental Hematology, 21(10), 1342-52.
Liesveld JL, et al. Phenotypic Characterization of the Human Fibrous Histiocytoma Giant Cell Tumor (GCT) Cell Line and Its Cytokine Repertoire. Exp Hematol. 1993;21(10):1342-52. PubMed PMID: 7689482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic characterization of the human fibrous histiocytoma giant cell tumor (GCT) cell line and its cytokine repertoire. AU - Liesveld,J L, AU - Rush,S, AU - Kempski,M C, AU - Turner,A R, AU - Brennan,J K, AU - Gasson,J C, AU - Abboud,C N, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 1342 EP - 52 JF - Experimental hematology JO - Exp Hematol VL - 21 IS - 10 N2 - The pleiotropic nature of malignant fibrous histiocytomas (MFH) is manifested as mixed cellular infiltrates consisting of myofibroblasts, histiomonocytes, and neutrophils. We detail in this report the phenotypic characteristics of the human fibrous histiocytoma giant cell tumor (GCT) cell line that establish its mesenchymal origin. The latter is underscored by the ability of GCT cells to express mRNA for transforming growth factor beta (TGF-beta) as well as both A and B chains of platelet-derived growth factor (PDGF). GCT cells also support the binding of CD34+ cells, but less efficiently than do normal marrow stromal cells. Since cytokines elaborated by MFH may mediate in part the recruitment of monocytes and neutrophils into tumor-infiltrated tissues, we have determined the cytokine repertoire of the GCT cell line, already known for its ability to elaborate colony-stimulating factors (CSFs) and interleukin-1 (IL-1). GCT cells express IL-1 alpha, IL-1 beta, IL-6, macrophage colony-stimulating factor (M-CSF or CSF-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), and IL-8. No detectable mRNA for IL-3, IL-4, IL-7, and tumor necrosis factor-alpha (TNF-alpha) was detected in GCT cells by polymerase chain reaction (PCR). Expression of cytokine mRNAs was responsive to agents such as dexamethasone (dex), 12-O-tetradecanoyl phorbol 13-acetate (phorbol diester or TPA), and TNF-alpha. Thus, this cell line provides a useful model for understanding the pathobiology of MFH and hematopoietic progenitor interactions with mesenchymal/stromal cells. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/7689482/Phenotypic_characterization_of_the_human_fibrous_histiocytoma_giant_cell_tumor__GCT__cell_line_and_its_cytokine_repertoire_ L2 - https://web.expasy.org/cellosaurus/CVCL_1229 DB - PRIME DP - Unbound Medicine ER -