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Detection of serum hepatitis C virus RNA in HCV antibody-seropositive volunteer blood donors.
Hepatology 1993; 18(3):485-90Hep

Abstract

Approximately 90% of subjects with chronic hepatitis resulting from hepatitis C virus infection have hepatitis C virus RNA in serum. However, the prevalence of hepatitis C virus RNA in serum from subjects with hepatitis C virus antibody associated with persistent normal liver biochemical values is unclear. Do these subjects have resolved or continuing infection with hepatitis C virus? The aim of this study was to examine whether subjects with hepatitis C virus antibody but normal ALT levels had evidence of ongoing infection. Our study population was divided into four groups. Groups 1, 2 and 3 comprised hepatitis C virus antibody-positive volunteer blood donors. Group 1 was made up of subjects found to be hepatitis C virus antibody-positive on enzyme-linked immunosorbent assay with persistent abnormal ALT levels (59 donors: 53 positive on recombinant immunoblot assay and 6 indeterminate). Group 2 members were hepatitis C virus antibody positive, with persistent normal ALT levels (50 donors: 39 positive on recombinant immunoblot assay and 11 indeterminate). Group 3 members were hepatitis C virus seropositive but negative on second-generation recombinant immunoblot assay (n = 48). Twenty patients (not blood donors) with chronic liver disease who were anti-hepatitis C virus seronegative were used as controls (group 4). Serum samples from all four groups were assayed for hepatitis C virus RNA on reverse transcription and a 40-cycle polymerase chain reaction with a combination of primers from the highly conserved 5'-noncoding and less-conserved third and fourth nonstructural regions. All assays were confirmed on hybridization with an internal probe.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

A.W. Morrow Gastroenterology and Liver Centre, Royal Prince Alfred Hospital, Camperdown NSW, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7689526

Citation

McGuinness, P H., et al. "Detection of Serum Hepatitis C Virus RNA in HCV Antibody-seropositive Volunteer Blood Donors." Hepatology (Baltimore, Md.), vol. 18, no. 3, 1993, pp. 485-90.
McGuinness PH, Bishop GA, Lien A, et al. Detection of serum hepatitis C virus RNA in HCV antibody-seropositive volunteer blood donors. Hepatology. 1993;18(3):485-90.
McGuinness, P. H., Bishop, G. A., Lien, A., Wiley, B., Parsons, C., & McCaughan, G. W. (1993). Detection of serum hepatitis C virus RNA in HCV antibody-seropositive volunteer blood donors. Hepatology (Baltimore, Md.), 18(3), pp. 485-90.
McGuinness PH, et al. Detection of Serum Hepatitis C Virus RNA in HCV Antibody-seropositive Volunteer Blood Donors. Hepatology. 1993;18(3):485-90. PubMed PMID: 7689526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of serum hepatitis C virus RNA in HCV antibody-seropositive volunteer blood donors. AU - McGuinness,P H, AU - Bishop,G A, AU - Lien,A, AU - Wiley,B, AU - Parsons,C, AU - McCaughan,G W, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 485 EP - 90 JF - Hepatology (Baltimore, Md.) JO - Hepatology VL - 18 IS - 3 N2 - Approximately 90% of subjects with chronic hepatitis resulting from hepatitis C virus infection have hepatitis C virus RNA in serum. However, the prevalence of hepatitis C virus RNA in serum from subjects with hepatitis C virus antibody associated with persistent normal liver biochemical values is unclear. Do these subjects have resolved or continuing infection with hepatitis C virus? The aim of this study was to examine whether subjects with hepatitis C virus antibody but normal ALT levels had evidence of ongoing infection. Our study population was divided into four groups. Groups 1, 2 and 3 comprised hepatitis C virus antibody-positive volunteer blood donors. Group 1 was made up of subjects found to be hepatitis C virus antibody-positive on enzyme-linked immunosorbent assay with persistent abnormal ALT levels (59 donors: 53 positive on recombinant immunoblot assay and 6 indeterminate). Group 2 members were hepatitis C virus antibody positive, with persistent normal ALT levels (50 donors: 39 positive on recombinant immunoblot assay and 11 indeterminate). Group 3 members were hepatitis C virus seropositive but negative on second-generation recombinant immunoblot assay (n = 48). Twenty patients (not blood donors) with chronic liver disease who were anti-hepatitis C virus seronegative were used as controls (group 4). Serum samples from all four groups were assayed for hepatitis C virus RNA on reverse transcription and a 40-cycle polymerase chain reaction with a combination of primers from the highly conserved 5'-noncoding and less-conserved third and fourth nonstructural regions. All assays were confirmed on hybridization with an internal probe.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0270-9139 UR - https://www.unboundmedicine.com/medline/citation/7689526/Detection_of_serum_hepatitis_C_virus_RNA_in_HCV_antibody_seropositive_volunteer_blood_donors_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0270-9139&date=1993&volume=18&issue=3&spage=485 DB - PRIME DP - Unbound Medicine ER -