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Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein.
J Clin Invest 1993; 92(3):1529-33JCI

Abstract

Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of core-gene sequences by polymerase chain reaction with type-specific primers. HCV genotypes were compared with various HCV-associated antibodies detectable by the first-generation ELISA (ELISA-1) with C100-3 protein and a second-generation immunoblot assay with four recombinant HCV proteins. Antibodies to C100-3 protein and those to its subsequence (5-1-1) were detected in 13 (93%) and 12 (86%), respectively, of 14 sera with genotype I HCV; 56 (79%) and 58 (82%) of 71 sera with genotype II; 13 (34%) and 6 (16%) of 38 sera with genotype III; and 11 (34%) and 4 (13%) of 32 sera with genotype IV. Amino acid sequences of C100-3 of genotype I HCV are conserved by approximately 90% in genotype II, but only by approximately 75% in genotypes III and IV. The sensitivity of ELISA-1, therefore, would be influenced by heterogeneity in C100-3 sequences of different genotypes.

Authors+Show Affiliations

First Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7690782

Citation

Nagayama, R, et al. "Genotype Dependence of Hepatitis C Virus Antibodies Detectable By the First-generation Enzyme-linked Immunosorbent Assay With C100-3 Protein." The Journal of Clinical Investigation, vol. 92, no. 3, 1993, pp. 1529-33.
Nagayama R, Tsuda F, Okamoto H, et al. Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein. J Clin Invest. 1993;92(3):1529-33.
Nagayama, R., Tsuda, F., Okamoto, H., Wang, Y., Mitsui, T., Tanaka, T., ... Mayumi, M. (1993). Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein. The Journal of Clinical Investigation, 92(3), pp. 1529-33.
Nagayama R, et al. Genotype Dependence of Hepatitis C Virus Antibodies Detectable By the First-generation Enzyme-linked Immunosorbent Assay With C100-3 Protein. J Clin Invest. 1993;92(3):1529-33. PubMed PMID: 7690782.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein. AU - Nagayama,R, AU - Tsuda,F, AU - Okamoto,H, AU - Wang,Y, AU - Mitsui,T, AU - Tanaka,T, AU - Miyakawa,Y, AU - Mayumi,M, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 1529 EP - 33 JF - The Journal of clinical investigation JO - J. Clin. Invest. VL - 92 IS - 3 N2 - Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of core-gene sequences by polymerase chain reaction with type-specific primers. HCV genotypes were compared with various HCV-associated antibodies detectable by the first-generation ELISA (ELISA-1) with C100-3 protein and a second-generation immunoblot assay with four recombinant HCV proteins. Antibodies to C100-3 protein and those to its subsequence (5-1-1) were detected in 13 (93%) and 12 (86%), respectively, of 14 sera with genotype I HCV; 56 (79%) and 58 (82%) of 71 sera with genotype II; 13 (34%) and 6 (16%) of 38 sera with genotype III; and 11 (34%) and 4 (13%) of 32 sera with genotype IV. Amino acid sequences of C100-3 of genotype I HCV are conserved by approximately 90% in genotype II, but only by approximately 75% in genotypes III and IV. The sensitivity of ELISA-1, therefore, would be influenced by heterogeneity in C100-3 sequences of different genotypes. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/7690782/Genotype_dependence_of_hepatitis_C_virus_antibodies_detectable_by_the_first_generation_enzyme_linked_immunosorbent_assay_with_C100_3_protein_ L2 - https://doi.org/10.1172/JCI116731 DB - PRIME DP - Unbound Medicine ER -