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Identification of phagocytosed Pneumocystis carinii in human pulmonary alveolar macrophages.
Cytopathology. 1993; 4(4):225-9.C

Abstract

Examination of Papanicolaou-stained bronchoalveolar lavage samples from cases with Pneumocystis carinii pneumonitis under ultra-violet light reveals alveolar macrophages packed with fluorescent inclusions. Immunoenzymatic staining of the alveolar macrophages with a monoclonal antibody specific for P. carinii (3F6) showed that these inclusions contain intact pneumocysts or their degradation products. Fluorescence microscopy of Papanicolaou-stained smears is advocated as a sensitive and specific method of diagnosing P. carinii infection.

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Authors+Show Affiliations

Wehle K
Department of Cytopathology, Heinrich Heine University, Düsseldorf, Germany.
Küpper T
No affiliation info available
Marzahn S
No affiliation info available
Pfitzer P
No affiliation info available

MeSH

Bronchoalveolar Lavage FluidHumansMacrophages, AlveolarMicroscopy, FluorescencePhagocytosisPneumocystisPneumonia, PneumocystisStaining and Labeling

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7691203

Citation

Wehle, K, et al. "Identification of Phagocytosed Pneumocystis Carinii in Human Pulmonary Alveolar Macrophages." Cytopathology : Official Journal of the British Society for Clinical Cytology, vol. 4, no. 4, 1993, pp. 225-9.
Wehle K, Küpper T, Marzahn S, et al. Identification of phagocytosed Pneumocystis carinii in human pulmonary alveolar macrophages. Cytopathology. 1993;4(4):225-9.
Wehle, K., Küpper, T., Marzahn, S., & Pfitzer, P. (1993). Identification of phagocytosed Pneumocystis carinii in human pulmonary alveolar macrophages. Cytopathology : Official Journal of the British Society for Clinical Cytology, 4(4), 225-9.
Wehle K, et al. Identification of Phagocytosed Pneumocystis Carinii in Human Pulmonary Alveolar Macrophages. Cytopathology. 1993;4(4):225-9. PubMed PMID: 7691203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of phagocytosed Pneumocystis carinii in human pulmonary alveolar macrophages. AU - Wehle,K, AU - Küpper,T, AU - Marzahn,S, AU - Pfitzer,P, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 225 EP - 9 JF - Cytopathology : official journal of the British Society for Clinical Cytology JO - Cytopathology VL - 4 IS - 4 N2 - Examination of Papanicolaou-stained bronchoalveolar lavage samples from cases with Pneumocystis carinii pneumonitis under ultra-violet light reveals alveolar macrophages packed with fluorescent inclusions. Immunoenzymatic staining of the alveolar macrophages with a monoclonal antibody specific for P. carinii (3F6) showed that these inclusions contain intact pneumocysts or their degradation products. Fluorescence microscopy of Papanicolaou-stained smears is advocated as a sensitive and specific method of diagnosing P. carinii infection. SN - 0956-5507 UR - https://www.unboundmedicine.com/medline/citation/7691203/Identification_of_phagocytosed_Pneumocystis_carinii_in_human_pulmonary_alveolar_macrophages_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0956-5507&date=1993&volume=4&issue=4&spage=225 DB - PRIME DP - Unbound Medicine ER -