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In vitro effect of stem cell factor on colony growth from acquired severe aplastic anemia.
Stem Cells. 1993 Jul; 11 Suppl 2:175-9.SC

Abstract

In this study we review our present understanding of the effect of stem cell factor (SCF) on the in vitro growth of hemopoietic progenitors from patients with acquired severe aplastic anemia (SAA). We have run three separate sets of experiments. First, we have tested the expression of receptor mRNAs for granulocyte-macrophage colony stimulating factor/interleukin 3 (GM-CSF/IL-3) and for c-kit protein on bone marrow (BM) cells from SAA patients. Molecular analysis revealed the presence of normal transcripts for alpha and beta chains of GM-CSF/IL-3 receptor and for c-kit protein by Northern blot analysis. Second, we have tested the in vitro response to SCF of BM cells derived from 11 SAA patients: SCF induced a significant enhancement of erythroid burst forming unit (BFU-E) growth (8 to 29, p = 0.01) and allowed the formation of granulocyte/erythroid/macrophage/megakaryocyte (GEMM) colonies which were not scored in baseline culture conditions (0 to 8, p = 0.01). Granulocyte-macrophage colony forming unit (CFU-GM) growth was also enhanced (4 to 20, p = 0.3). This was true for patients both at diagnosis and after antilymphocyte globulin (ALG) treatment. We concluded that SCF can promote the in vitro growth of hemopoietic progenitors in patients with acquired SAA. Third, we have tested the response to SCF of peripheral blood (PB) hemopoietic progenitors collected from patients receiving in vivo long-term treatment with granulocyte CSF (G-CSF). When PB cells were plated directly in the presence of GM-CSF there was no colony formation.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Divisione di Ematologia, Ospedale San Martino, Genova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7691324

Citation

Bacigalupo, A, et al. "In Vitro Effect of Stem Cell Factor On Colony Growth From Acquired Severe Aplastic Anemia." Stem Cells (Dayton, Ohio), vol. 11 Suppl 2, 1993, pp. 175-9.
Bacigalupo A, Piaggio G, Podestá M, et al. In vitro effect of stem cell factor on colony growth from acquired severe aplastic anemia. Stem Cells. 1993;11 Suppl 2:175-9.
Bacigalupo, A., Piaggio, G., Podestá, M., Raffo, M. R., Tedone, E., Sogno, G., Benvenuto, F., Figari, O., Grassia, L., & Bagnara, G. P. (1993). In vitro effect of stem cell factor on colony growth from acquired severe aplastic anemia. Stem Cells (Dayton, Ohio), 11 Suppl 2, 175-9.
Bacigalupo A, et al. In Vitro Effect of Stem Cell Factor On Colony Growth From Acquired Severe Aplastic Anemia. Stem Cells. 1993;11 Suppl 2:175-9. PubMed PMID: 7691324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro effect of stem cell factor on colony growth from acquired severe aplastic anemia. A1 - Bacigalupo,A, AU - Piaggio,G, AU - Podestá,M, AU - Raffo,M R, AU - Tedone,E, AU - Sogno,G, AU - Benvenuto,F, AU - Figari,O, AU - Grassia,L, AU - Bagnara,G P, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 175 EP - 9 JF - Stem cells (Dayton, Ohio) JO - Stem Cells VL - 11 Suppl 2 N2 - In this study we review our present understanding of the effect of stem cell factor (SCF) on the in vitro growth of hemopoietic progenitors from patients with acquired severe aplastic anemia (SAA). We have run three separate sets of experiments. First, we have tested the expression of receptor mRNAs for granulocyte-macrophage colony stimulating factor/interleukin 3 (GM-CSF/IL-3) and for c-kit protein on bone marrow (BM) cells from SAA patients. Molecular analysis revealed the presence of normal transcripts for alpha and beta chains of GM-CSF/IL-3 receptor and for c-kit protein by Northern blot analysis. Second, we have tested the in vitro response to SCF of BM cells derived from 11 SAA patients: SCF induced a significant enhancement of erythroid burst forming unit (BFU-E) growth (8 to 29, p = 0.01) and allowed the formation of granulocyte/erythroid/macrophage/megakaryocyte (GEMM) colonies which were not scored in baseline culture conditions (0 to 8, p = 0.01). Granulocyte-macrophage colony forming unit (CFU-GM) growth was also enhanced (4 to 20, p = 0.3). This was true for patients both at diagnosis and after antilymphocyte globulin (ALG) treatment. We concluded that SCF can promote the in vitro growth of hemopoietic progenitors in patients with acquired SAA. Third, we have tested the response to SCF of peripheral blood (PB) hemopoietic progenitors collected from patients receiving in vivo long-term treatment with granulocyte CSF (G-CSF). When PB cells were plated directly in the presence of GM-CSF there was no colony formation.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 1066-5099 UR - https://www.unboundmedicine.com/medline/citation/7691324/In_vitro_effect_of_stem_cell_factor_on_colony_growth_from_acquired_severe_aplastic_anemia_ L2 - https://doi.org/10.1002/stem.5530110828 DB - PRIME DP - Unbound Medicine ER -