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Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand.
Mutat Res. 1993 Nov; 303(3):135-42.MR

Abstract

Three kinds of spices (caraway, coriander and black pepper seeds) and a medicinal plant called 'tong tak' in Thai (Baliospermum axillar, a species of the spurge family) were fractionated into hot water, methanol and hexane extracts. These extracts were not mutagenic for Salmonella typhimurium strains TA98 and TA100 by the Ames assay. However, when the extracts were treated with nitrite, samples of the water and methanol extracts were mutagenic for strain TA100 without metabolic activation. The mutagenicity of the nitrite-treated methanol and hot water extracts of black pepper was highest (8380 and 22,200 His+ per 0.1 g of spice powder, respectively), and that of the nitrite-treated hot water extracts of caraway and tong tak was moderate. The hot water extracts were examined for their antimutagenic activity against mutagenicity induced by various carcinogens by the Ames assay, using the preincubation technique. The tested samples (equivalent to 1-2 mg of spice powder) reduced the mutagenicity induced by 2.7 nmole (397 ng) of N-methyl-N'-nitro-N-nitrosoguanidine by more than 84%, and that induced by dimethylnitrosamine (1.48 mg) or ICR-170 (10 ng) by 30-60%. However, they did not inhibit the mutagenic activity of 1-nitropyrene, 3-nitrofluoranthene, AF-2, methyl methanesulfonate, N-ethyl-N'-nitro-N-nitrosoguanidine, 2-aminoanthracene, 2-acetylaminofluorene, benzo[a]pyrene or IQ.

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Authors+Show Affiliations

Higashimoto M
Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
Purintrapiban J
No affiliation info available
Kataoka K
No affiliation info available
Kinouchi T
No affiliation info available
Vinitketkumnuen U
No affiliation info available
Akimoto S
No affiliation info available
Matsumoto H
No affiliation info available
Ohnishi Y
No affiliation info available

MeSH

Antimutagenic AgentsMethylnitronitrosoguanidineMutagenicity TestsMutagensNitritesPlant ExtractsPlants, MedicinalSalmonella typhimuriumSpicesThailand

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7694130

Citation

Higashimoto, M, et al. "Mutagenicity and Antimutagenicity of Extracts of Three Spices and a Medicinal Plant in Thailand." Mutation Research, vol. 303, no. 3, 1993, pp. 135-42.
Higashimoto M, Purintrapiban J, Kataoka K, et al. Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand. Mutat Res. 1993;303(3):135-42.
Higashimoto, M., Purintrapiban, J., Kataoka, K., Kinouchi, T., Vinitketkumnuen, U., Akimoto, S., Matsumoto, H., & Ohnishi, Y. (1993). Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand. Mutation Research, 303(3), 135-42.
Higashimoto M, et al. Mutagenicity and Antimutagenicity of Extracts of Three Spices and a Medicinal Plant in Thailand. Mutat Res. 1993;303(3):135-42. PubMed PMID: 7694130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand. AU - Higashimoto,M, AU - Purintrapiban,J, AU - Kataoka,K, AU - Kinouchi,T, AU - Vinitketkumnuen,U, AU - Akimoto,S, AU - Matsumoto,H, AU - Ohnishi,Y, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 135 EP - 42 JF - Mutation research JO - Mutat Res VL - 303 IS - 3 N2 - Three kinds of spices (caraway, coriander and black pepper seeds) and a medicinal plant called 'tong tak' in Thai (Baliospermum axillar, a species of the spurge family) were fractionated into hot water, methanol and hexane extracts. These extracts were not mutagenic for Salmonella typhimurium strains TA98 and TA100 by the Ames assay. However, when the extracts were treated with nitrite, samples of the water and methanol extracts were mutagenic for strain TA100 without metabolic activation. The mutagenicity of the nitrite-treated methanol and hot water extracts of black pepper was highest (8380 and 22,200 His+ per 0.1 g of spice powder, respectively), and that of the nitrite-treated hot water extracts of caraway and tong tak was moderate. The hot water extracts were examined for their antimutagenic activity against mutagenicity induced by various carcinogens by the Ames assay, using the preincubation technique. The tested samples (equivalent to 1-2 mg of spice powder) reduced the mutagenicity induced by 2.7 nmole (397 ng) of N-methyl-N'-nitro-N-nitrosoguanidine by more than 84%, and that induced by dimethylnitrosamine (1.48 mg) or ICR-170 (10 ng) by 30-60%. However, they did not inhibit the mutagenic activity of 1-nitropyrene, 3-nitrofluoranthene, AF-2, methyl methanesulfonate, N-ethyl-N'-nitro-N-nitrosoguanidine, 2-aminoanthracene, 2-acetylaminofluorene, benzo[a]pyrene or IQ. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/7694130/Mutagenicity_and_antimutagenicity_of_extracts_of_three_spices_and_a_medicinal_plant_in_Thailand_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0165-7992(93)90026-R DB - PRIME DP - Unbound Medicine ER -