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Influence of bile on beta-galactosidase activity of component species of yogurt starter cultures.
J Dairy Sci. 1994 Dec; 77(12):3532-7.JD

Abstract

The influence of bile on beta-galactosidase activity and the cellular integrity of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus were tested. Two strains (OS 1 and OS 2) of L. delbrueckii ssp. bulgaricus and three strains (HC 15, 17, and 18) of S. salivarius ssp. thermophilus were studied. In the presence of .15% oxgall, beta-galactosidase activity of whole cells of both species was significantly increased. However, at a higher concentration (.3% oxgall), the beta-galactosidase activity was significantly less than in the presence of .15% oxgall. The presence of oxgall did not promote the cell lysis of any of the strains of either species. Additionally, the presence of oxgall did not cause the leakage of the enzyme from the cells. Thus, the presence of oxgall increased the cellular permeability to allow more substrate to enter the cells, thereby increasing beta-galactosidase activity, and, at higher concentrations, was inhibitory to beta-galactosidase activity of both species.

Authors+Show Affiliations

Department of Animal Science, Oklahoma State University, Stillwater 74078.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7699132

Citation

Noh, D O., and S E. Gilliland. "Influence of Bile On Beta-galactosidase Activity of Component Species of Yogurt Starter Cultures." Journal of Dairy Science, vol. 77, no. 12, 1994, pp. 3532-7.
Noh DO, Gilliland SE. Influence of bile on beta-galactosidase activity of component species of yogurt starter cultures. J Dairy Sci. 1994;77(12):3532-7.
Noh, D. O., & Gilliland, S. E. (1994). Influence of bile on beta-galactosidase activity of component species of yogurt starter cultures. Journal of Dairy Science, 77(12), 3532-7.
Noh DO, Gilliland SE. Influence of Bile On Beta-galactosidase Activity of Component Species of Yogurt Starter Cultures. J Dairy Sci. 1994;77(12):3532-7. PubMed PMID: 7699132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of bile on beta-galactosidase activity of component species of yogurt starter cultures. AU - Noh,D O, AU - Gilliland,S E, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 3532 EP - 7 JF - Journal of dairy science JO - J Dairy Sci VL - 77 IS - 12 N2 - The influence of bile on beta-galactosidase activity and the cellular integrity of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus were tested. Two strains (OS 1 and OS 2) of L. delbrueckii ssp. bulgaricus and three strains (HC 15, 17, and 18) of S. salivarius ssp. thermophilus were studied. In the presence of .15% oxgall, beta-galactosidase activity of whole cells of both species was significantly increased. However, at a higher concentration (.3% oxgall), the beta-galactosidase activity was significantly less than in the presence of .15% oxgall. The presence of oxgall did not promote the cell lysis of any of the strains of either species. Additionally, the presence of oxgall did not cause the leakage of the enzyme from the cells. Thus, the presence of oxgall increased the cellular permeability to allow more substrate to enter the cells, thereby increasing beta-galactosidase activity, and, at higher concentrations, was inhibitory to beta-galactosidase activity of both species. SN - 0022-0302 UR - https://www.unboundmedicine.com/medline/citation/7699132/Influence_of_bile_on_beta_galactosidase_activity_of_component_species_of_yogurt_starter_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(94)77295-7 DB - PRIME DP - Unbound Medicine ER -