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Factors affecting the ability of a high beta-galactosidase yogurt to enhance lactose absorption.
J Dairy Sci 1994; 77(12):3538-44JD

Abstract

Lactose in yogurt is better absorbed by lactase-deficient subjects than is an equivalent quantity of lactose in milk, presumably because of the microbial activity of the beta-galactosidase present in yogurt. In this study, we describe a process that increases the beta-galactosidase of yogurt 5- to 6-fold and the ability of this high lactase yogurt to enhance lactose absorption in lactase-deficient subjects. These subjects ingested the yogurt meals after a 12-h fast, and lactose malabsorption was determined by measuring breath hydrogen. Breath hydrogen was reduced 39% following ingestion of high lactase yogurt from that after consumption of conventional yogurt, indicating that the high lactase yogurt enhanced lactose absorption. However, the reduction after high lactase yogurt was less than expected, given the 5- to 6-fold increment in beta-galactosidase measured in vitro. In vivo activity of beta-galactosidase requires that the enzyme resist acid denaturation in the stomach. The beta-galactosidase in high lactase yogurt was much less acid resistant than was the beta-galactosidase in conventional yogurt, and the relative inability of high lactase yogurt to enhance lactose absorption was likely due to the destruction of the beta-galactosidase in the stomach.

Authors+Show Affiliations

University of Minnesota, Department of Food Science and Nutrition, St. Paul 55108.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7699133

Citation

Kotz, C M., et al. "Factors Affecting the Ability of a High Beta-galactosidase Yogurt to Enhance Lactose Absorption." Journal of Dairy Science, vol. 77, no. 12, 1994, pp. 3538-44.
Kotz CM, Furne JK, Savaiano DA, et al. Factors affecting the ability of a high beta-galactosidase yogurt to enhance lactose absorption. J Dairy Sci. 1994;77(12):3538-44.
Kotz, C. M., Furne, J. K., Savaiano, D. A., & Levitt, M. D. (1994). Factors affecting the ability of a high beta-galactosidase yogurt to enhance lactose absorption. Journal of Dairy Science, 77(12), pp. 3538-44.
Kotz CM, et al. Factors Affecting the Ability of a High Beta-galactosidase Yogurt to Enhance Lactose Absorption. J Dairy Sci. 1994;77(12):3538-44. PubMed PMID: 7699133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Factors affecting the ability of a high beta-galactosidase yogurt to enhance lactose absorption. AU - Kotz,C M, AU - Furne,J K, AU - Savaiano,D A, AU - Levitt,M D, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 3538 EP - 44 JF - Journal of dairy science JO - J. Dairy Sci. VL - 77 IS - 12 N2 - Lactose in yogurt is better absorbed by lactase-deficient subjects than is an equivalent quantity of lactose in milk, presumably because of the microbial activity of the beta-galactosidase present in yogurt. In this study, we describe a process that increases the beta-galactosidase of yogurt 5- to 6-fold and the ability of this high lactase yogurt to enhance lactose absorption in lactase-deficient subjects. These subjects ingested the yogurt meals after a 12-h fast, and lactose malabsorption was determined by measuring breath hydrogen. Breath hydrogen was reduced 39% following ingestion of high lactase yogurt from that after consumption of conventional yogurt, indicating that the high lactase yogurt enhanced lactose absorption. However, the reduction after high lactase yogurt was less than expected, given the 5- to 6-fold increment in beta-galactosidase measured in vitro. In vivo activity of beta-galactosidase requires that the enzyme resist acid denaturation in the stomach. The beta-galactosidase in high lactase yogurt was much less acid resistant than was the beta-galactosidase in conventional yogurt, and the relative inability of high lactase yogurt to enhance lactose absorption was likely due to the destruction of the beta-galactosidase in the stomach. SN - 0022-0302 UR - https://www.unboundmedicine.com/medline/citation/7699133/Factors_affecting_the_ability_of_a_high_beta_galactosidase_yogurt_to_enhance_lactose_absorption_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(94)77296-9 DB - PRIME DP - Unbound Medicine ER -