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The susceptibility of low-density lipoprotein to in vitro oxidation is increased in hypercholesterolemic patients.
Nutrition 1994 Nov-Dec; 10(6):527-31N

Abstract

It has been suggested that the oxidative modification of low-density lipoprotein (LDL) plays a major role in atherogenesis. We evaluated the oxidative resistance to copper-induced oxidative changes of LDL derived from patients affected by type IIa hyperlipoproteinemia compared with healthy subjects and faced the question of the importance of the antioxidants and polyunsaturated fatty acids (PUFAs) contained in LDL in determining its variability. LDL isolated from the plasmas of 25 subjects affected by familial hypercholesterolemia and 15 control subjects was oxidatively modified with Cu2+ in vitro, and the differences in LDL susceptibilities (lag and propagation phases) to lipid peroxidation were studied by measuring the changes in fluorescence intensity. LDL alpha-tocopherol and PUFAs were also measured. The lag phase was significantly lower and the propagation phase significantly higher in the type IIa patients than in control subjects (p < 0.01). The linoleic and arachidonic acids, expressed as percentage of total LDL fatty acids, were significantly higher in type IIa patients than in the control subjects (p < 0.01). There was a positive significant correlation between the LDL cholesterol and the linoleic and arachidonic acids as percentage of total LDL fatty acids (p < 0.01). Both linoleic and arachidonic acids turned out to be negatively correlated with the lag phase and positively with the propagation phase (p < 0.01). The concentration of LDL alpha-tocopherol was similar in the two groups. Therefore, type IIa patients have a greater susceptibility to LDL oxidation than control subjects. This may be due to a relative higher concentration of linoleic and arachidonic acids in LDL derived from patients with familial hypercholesterolemia.

Authors+Show Affiliations

Istituto di Semeiotica e Nefrologia Medica, Università di Verona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

7703599

Citation

Cominacini, L, et al. "The Susceptibility of Low-density Lipoprotein to in Vitro Oxidation Is Increased in Hypercholesterolemic Patients." Nutrition (Burbank, Los Angeles County, Calif.), vol. 10, no. 6, 1994, pp. 527-31.
Cominacini L, Pastorino AM, Garbin U, et al. The susceptibility of low-density lipoprotein to in vitro oxidation is increased in hypercholesterolemic patients. Nutrition. 1994;10(6):527-31.
Cominacini, L., Pastorino, A. M., Garbin, U., Campagnola, M., de Santis, A., Davoli, A., ... Pasini, A. F. (1994). The susceptibility of low-density lipoprotein to in vitro oxidation is increased in hypercholesterolemic patients. Nutrition (Burbank, Los Angeles County, Calif.), 10(6), pp. 527-31.
Cominacini L, et al. The Susceptibility of Low-density Lipoprotein to in Vitro Oxidation Is Increased in Hypercholesterolemic Patients. Nutrition. 1994;10(6):527-31. PubMed PMID: 7703599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The susceptibility of low-density lipoprotein to in vitro oxidation is increased in hypercholesterolemic patients. A1 - Cominacini,L, AU - Pastorino,A M, AU - Garbin,U, AU - Campagnola,M, AU - de Santis,A, AU - Davoli,A, AU - Faccini,G, AU - Bertozzo,L, AU - Pasini,F, AU - Pasini,A F, PY - 1994/11/1/pubmed PY - 1994/11/1/medline PY - 1994/11/1/entrez SP - 527 EP - 31 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 10 IS - 6 N2 - It has been suggested that the oxidative modification of low-density lipoprotein (LDL) plays a major role in atherogenesis. We evaluated the oxidative resistance to copper-induced oxidative changes of LDL derived from patients affected by type IIa hyperlipoproteinemia compared with healthy subjects and faced the question of the importance of the antioxidants and polyunsaturated fatty acids (PUFAs) contained in LDL in determining its variability. LDL isolated from the plasmas of 25 subjects affected by familial hypercholesterolemia and 15 control subjects was oxidatively modified with Cu2+ in vitro, and the differences in LDL susceptibilities (lag and propagation phases) to lipid peroxidation were studied by measuring the changes in fluorescence intensity. LDL alpha-tocopherol and PUFAs were also measured. The lag phase was significantly lower and the propagation phase significantly higher in the type IIa patients than in control subjects (p < 0.01). The linoleic and arachidonic acids, expressed as percentage of total LDL fatty acids, were significantly higher in type IIa patients than in the control subjects (p < 0.01). There was a positive significant correlation between the LDL cholesterol and the linoleic and arachidonic acids as percentage of total LDL fatty acids (p < 0.01). Both linoleic and arachidonic acids turned out to be negatively correlated with the lag phase and positively with the propagation phase (p < 0.01). The concentration of LDL alpha-tocopherol was similar in the two groups. Therefore, type IIa patients have a greater susceptibility to LDL oxidation than control subjects. This may be due to a relative higher concentration of linoleic and arachidonic acids in LDL derived from patients with familial hypercholesterolemia. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/7703599/The_susceptibility_of_low_density_lipoprotein_to_in_vitro_oxidation_is_increased_in_hypercholesterolemic_patients_ L2 - https://medlineplus.gov/cholesterollevelswhatyouneedtoknow.html DB - PRIME DP - Unbound Medicine ER -