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Small volume bronchoalveolar lavage used in diagnosing Pneumocystis carinii pneumonia in HIV-infected patients.
Chest. 1995 Apr; 107(4):1013-7.Chest

Abstract

To determine the volume of bronchoalveolar lavage (BAL) fluid necessary to diagnose Pneumocystis carinii pneumonia (PCP) in immunocompromised patients, specimens from 25 patients were evaluated. Twenty-one patients were HIV infected. BAL was performed using three to four 60-mL aliquots of room temperature, sterile, saline solution. Each syringe of BAL effluent was numbered and its volume was measured. Immunofluorescent stains were performed on about 8-mL aliquots of the initial, final, and aggregate BAL specimens, and a modified Giemsa stain was also performed on a 0.4-mL aliquot of the aggregate specimen. Of 25 patients, Pneumocystis carinii organisms were identified in 9 with HIV infection, in whom all BAL specimens were positive with both immunofluorescence and Giemsa stains. In 16 patients, BAL specimens were negative for P carinii on both immunofluorescent and modified Giemsa testing. Both staining methods were 100% specific (95% confidence interval [CI], 83 to 100%) and 100% sensitive (95% CI, 72 to 100%). The volume of BAL effluent in the initial specimens positive for P carinii ranged from 15 to 25 mL. We conclude that in this small group of patients, PCP was accurately diagnosed from a single 60-mL BAL specimen stained with immunofluorescence methods.

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Authors+Show Affiliations

Torrington KG
Pulmonary and Critical Care Medicine Service, Walter Reed Army Medical Center, Washington, DC, 20307, USA.
Finelli MR
No affiliation info available

MeSH

AIDS-Related Opportunistic InfectionsAdultAgedAzure StainsBronchoalveolar Lavage FluidFemaleFluorescent Antibody TechniqueHumansMaleMiddle AgedPneumonia, PneumocystisSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7705107

Citation

Torrington, K G., and M R. Finelli. "Small Volume Bronchoalveolar Lavage Used in Diagnosing Pneumocystis Carinii Pneumonia in HIV-infected Patients." Chest, vol. 107, no. 4, 1995, pp. 1013-7.
Torrington KG, Finelli MR. Small volume bronchoalveolar lavage used in diagnosing Pneumocystis carinii pneumonia in HIV-infected patients. Chest. 1995;107(4):1013-7.
Torrington, K. G., & Finelli, M. R. (1995). Small volume bronchoalveolar lavage used in diagnosing Pneumocystis carinii pneumonia in HIV-infected patients. Chest, 107(4), 1013-7.
Torrington KG, Finelli MR. Small Volume Bronchoalveolar Lavage Used in Diagnosing Pneumocystis Carinii Pneumonia in HIV-infected Patients. Chest. 1995;107(4):1013-7. PubMed PMID: 7705107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Small volume bronchoalveolar lavage used in diagnosing Pneumocystis carinii pneumonia in HIV-infected patients. AU - Torrington,K G, AU - Finelli,M R, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 1013 EP - 7 JF - Chest JO - Chest VL - 107 IS - 4 N2 - To determine the volume of bronchoalveolar lavage (BAL) fluid necessary to diagnose Pneumocystis carinii pneumonia (PCP) in immunocompromised patients, specimens from 25 patients were evaluated. Twenty-one patients were HIV infected. BAL was performed using three to four 60-mL aliquots of room temperature, sterile, saline solution. Each syringe of BAL effluent was numbered and its volume was measured. Immunofluorescent stains were performed on about 8-mL aliquots of the initial, final, and aggregate BAL specimens, and a modified Giemsa stain was also performed on a 0.4-mL aliquot of the aggregate specimen. Of 25 patients, Pneumocystis carinii organisms were identified in 9 with HIV infection, in whom all BAL specimens were positive with both immunofluorescence and Giemsa stains. In 16 patients, BAL specimens were negative for P carinii on both immunofluorescent and modified Giemsa testing. Both staining methods were 100% specific (95% confidence interval [CI], 83 to 100%) and 100% sensitive (95% CI, 72 to 100%). The volume of BAL effluent in the initial specimens positive for P carinii ranged from 15 to 25 mL. We conclude that in this small group of patients, PCP was accurately diagnosed from a single 60-mL BAL specimen stained with immunofluorescence methods. SN - 0012-3692 UR - https://www.unboundmedicine.com/medline/citation/7705107/Small_volume_bronchoalveolar_lavage_used_in_diagnosing_Pneumocystis_carinii_pneumonia_in_HIV_infected_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-3692(16)33218-4 DB - PRIME DP - Unbound Medicine ER -