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Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study.
Wien Klin Wochenschr 1995; 107(4):133-6WK

Abstract

The microparticle enzyme immunoassay (MEIA) for detection of Toxoplasma specific IgG antibodies was compared to the Sabin-Feldman dye test (DT) as reference in 843 serum samples from a cohort of 757 pregnant women. The overall correlation for individual measurements was highly significant (R = 0.9446, p < 0.0001). DT and specific IgM combined allowed definition of 3 groups of patients: group 1 (no infection), group 2 (latent infection), group 3 (acute infection). A significant difference was found between the groups for the corresponding IgG values, as determined by the MEIA method, which allowed the following cut-off points to be laid down: group 1: 0-5.2 IU/ml, group 2: 5.3-187.5, and group 3: 187.6 IU/ml and higher. The validity of the cut-off points was tested in a subgroup of 57 patients who underwent serological follow-up during pregnancy. All 15 acutely- and 14 non-infected women, as well as 25 out of 28 latent infections were identified correctly. 3 latent infection were allocated falsely as acute. The threshold values presented in this report need to be confirmed in a large prospective study.

Authors+Show Affiliations

Department of Pediatrics, University of Vienna.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7709628

Citation

Hayde, M, et al. "Microparticle Enzyme Immunoassay (MEIA) for Toxoplasma Specific Immunoglobulin G in Comparison to the Sabin-Feldman Dye Test. a Pilot Study." Wiener Klinische Wochenschrift, vol. 107, no. 4, 1995, pp. 133-6.
Hayde M, Salzer HR, Gittler G, et al. Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study. Wien Klin Wochenschr. 1995;107(4):133-6.
Hayde, M., Salzer, H. R., Gittler, G., Aspöck, H., & Pollak, A. (1995). Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study. Wiener Klinische Wochenschrift, 107(4), pp. 133-6.
Hayde M, et al. Microparticle Enzyme Immunoassay (MEIA) for Toxoplasma Specific Immunoglobulin G in Comparison to the Sabin-Feldman Dye Test. a Pilot Study. Wien Klin Wochenschr. 1995;107(4):133-6. PubMed PMID: 7709628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study. AU - Hayde,M, AU - Salzer,H R, AU - Gittler,G, AU - Aspöck,H, AU - Pollak,A, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 133 EP - 6 JF - Wiener klinische Wochenschrift JO - Wien. Klin. Wochenschr. VL - 107 IS - 4 N2 - The microparticle enzyme immunoassay (MEIA) for detection of Toxoplasma specific IgG antibodies was compared to the Sabin-Feldman dye test (DT) as reference in 843 serum samples from a cohort of 757 pregnant women. The overall correlation for individual measurements was highly significant (R = 0.9446, p < 0.0001). DT and specific IgM combined allowed definition of 3 groups of patients: group 1 (no infection), group 2 (latent infection), group 3 (acute infection). A significant difference was found between the groups for the corresponding IgG values, as determined by the MEIA method, which allowed the following cut-off points to be laid down: group 1: 0-5.2 IU/ml, group 2: 5.3-187.5, and group 3: 187.6 IU/ml and higher. The validity of the cut-off points was tested in a subgroup of 57 patients who underwent serological follow-up during pregnancy. All 15 acutely- and 14 non-infected women, as well as 25 out of 28 latent infections were identified correctly. 3 latent infection were allocated falsely as acute. The threshold values presented in this report need to be confirmed in a large prospective study. SN - 0043-5325 UR - https://www.unboundmedicine.com/medline/citation/7709628/Microparticle_enzyme_immunoassay__MEIA__for_toxoplasma_specific_immunoglobulin_G_in_comparison_to_the_Sabin_Feldman_dye_test__A_pilot_study_ L2 - https://medlineplus.gov/toxoplasmosis.html DB - PRIME DP - Unbound Medicine ER -