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Comparative assessment of viability of M.leprae by mouse foot-pad and fluorescent staining techniques.
Indian J Lepr. 1994 Oct-Dec; 66(4):455-62.IJ

Abstract

Morphological characteristics have been used as a parameter to assess the viability of M.leprae in leprosy patients. However, with the advent of the mouse foot-pad technique, viability of M.leprae is determined by growing the bacilli in the mouse foot-pad. In recent years, a fluorescent staining technique using fluorescent diacetate-ethidium bromide (FDA-EB) has been used to assess the viability of cultivable mycobacteria as well as M.leprae. The purpose of this study was to compare the viability of M.leprae by both mouse foot-pad and fluorescent staining techniques. M.leprae strains from both untreated and treated patients as well as mouse passaged strains of M.leprae were used for the comparison. Percentage of green-stained bacilli in the inoculum was compared with that of multiplication of M.leprae in the mouse foot-pad. It was observed that there was no correlation between the estimates of viable M.leprae by fluorescent staining and by mouse foot-pad inoculation. FDA-EB staining appears to reflect only trends as absence of green staining cells had overall general correlation with loss of infectivity to mouse foot-pad but, the converse was not found to be true.

Authors+Show Affiliations

CJIL Field Unit (ICMR), Madras.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7714355

Citation

Sreevatsa, , and V M. Katoch. "Comparative Assessment of Viability of M.leprae By Mouse Foot-pad and Fluorescent Staining Techniques." Indian Journal of Leprosy, vol. 66, no. 4, 1994, pp. 455-62.
Sreevatsa , Katoch VM. Comparative assessment of viability of M.leprae by mouse foot-pad and fluorescent staining techniques. Indian J Lepr. 1994;66(4):455-62.
Sreevatsa, ., & Katoch, V. M. (1994). Comparative assessment of viability of M.leprae by mouse foot-pad and fluorescent staining techniques. Indian Journal of Leprosy, 66(4), 455-62.
Sreevatsa , Katoch VM. Comparative Assessment of Viability of M.leprae By Mouse Foot-pad and Fluorescent Staining Techniques. Indian J Lepr. 1994 Oct-Dec;66(4):455-62. PubMed PMID: 7714355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative assessment of viability of M.leprae by mouse foot-pad and fluorescent staining techniques. AU - Sreevatsa,, AU - Katoch,V M, PY - 1994/10/1/pubmed PY - 1994/10/1/medline PY - 1994/10/1/entrez SP - 455 EP - 62 JF - Indian journal of leprosy JO - Indian J Lepr VL - 66 IS - 4 N2 - Morphological characteristics have been used as a parameter to assess the viability of M.leprae in leprosy patients. However, with the advent of the mouse foot-pad technique, viability of M.leprae is determined by growing the bacilli in the mouse foot-pad. In recent years, a fluorescent staining technique using fluorescent diacetate-ethidium bromide (FDA-EB) has been used to assess the viability of cultivable mycobacteria as well as M.leprae. The purpose of this study was to compare the viability of M.leprae by both mouse foot-pad and fluorescent staining techniques. M.leprae strains from both untreated and treated patients as well as mouse passaged strains of M.leprae were used for the comparison. Percentage of green-stained bacilli in the inoculum was compared with that of multiplication of M.leprae in the mouse foot-pad. It was observed that there was no correlation between the estimates of viable M.leprae by fluorescent staining and by mouse foot-pad inoculation. FDA-EB staining appears to reflect only trends as absence of green staining cells had overall general correlation with loss of infectivity to mouse foot-pad but, the converse was not found to be true. SN - 0254-9395 UR - https://www.unboundmedicine.com/medline/citation/7714355/Comparative_assessment_of_viability_of_M_leprae_by_mouse_foot_pad_and_fluorescent_staining_techniques_ DB - PRIME DP - Unbound Medicine ER -
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