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Combinatorial signals from the neural tube, floor plate and notochord induce myogenic bHLH gene expression in the somite.
Development. 1995 Mar; 121(3):651-60.D

Abstract

The neural tube, floor plate and notochord are axial tissues in the vertebrate embryo which have been demonstrated to play a role in somite morphogenesis. Using in vitro coculture of tissue explants, we have monitored inductive interactions of these axial tissues with the adjacent somitic mesoderm in chick embryos. We have found that signals from the neural tube and floor plate/notochord are necessary for expression of the myogenic bHLH regulators MyoD, Myf5 and myogenin in the somite. Eventually somitic expression of the myogenic bHLH genes is maintained in the absence of the axial tissues. In organ culture, at early developmental stages (HH 11-), induction of myogenesis in the three most recently formed somites can be mediated by the neural tube together with the floor plate/notochord, while in more rostral somites (stages IV-IX) the neural tube without the floor plate/notochord is sufficient. By recombining somites and neural tubes from different axial levels of the embryo, we have found that a second signal is necessary to promote competence of the somite to respond to inducing signals from the neural tube. Thus, we propose that at least two signals from axial tissues work in combination to induce myogenic bHLH gene expression; one signal derives from the floor plate/notochord and the other signal derives from regions of the neural tube other than the floor plate.

Authors+Show Affiliations

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7720573

Citation

Münsterberg, A E., and A B. Lassar. "Combinatorial Signals From the Neural Tube, Floor Plate and Notochord Induce Myogenic bHLH Gene Expression in the Somite." Development (Cambridge, England), vol. 121, no. 3, 1995, pp. 651-60.
Münsterberg AE, Lassar AB. Combinatorial signals from the neural tube, floor plate and notochord induce myogenic bHLH gene expression in the somite. Development. 1995;121(3):651-60.
Münsterberg, A. E., & Lassar, A. B. (1995). Combinatorial signals from the neural tube, floor plate and notochord induce myogenic bHLH gene expression in the somite. Development (Cambridge, England), 121(3), 651-60.
Münsterberg AE, Lassar AB. Combinatorial Signals From the Neural Tube, Floor Plate and Notochord Induce Myogenic bHLH Gene Expression in the Somite. Development. 1995;121(3):651-60. PubMed PMID: 7720573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combinatorial signals from the neural tube, floor plate and notochord induce myogenic bHLH gene expression in the somite. AU - Münsterberg,A E, AU - Lassar,A B, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 651 EP - 60 JF - Development (Cambridge, England) JO - Development VL - 121 IS - 3 N2 - The neural tube, floor plate and notochord are axial tissues in the vertebrate embryo which have been demonstrated to play a role in somite morphogenesis. Using in vitro coculture of tissue explants, we have monitored inductive interactions of these axial tissues with the adjacent somitic mesoderm in chick embryos. We have found that signals from the neural tube and floor plate/notochord are necessary for expression of the myogenic bHLH regulators MyoD, Myf5 and myogenin in the somite. Eventually somitic expression of the myogenic bHLH genes is maintained in the absence of the axial tissues. In organ culture, at early developmental stages (HH 11-), induction of myogenesis in the three most recently formed somites can be mediated by the neural tube together with the floor plate/notochord, while in more rostral somites (stages IV-IX) the neural tube without the floor plate/notochord is sufficient. By recombining somites and neural tubes from different axial levels of the embryo, we have found that a second signal is necessary to promote competence of the somite to respond to inducing signals from the neural tube. Thus, we propose that at least two signals from axial tissues work in combination to induce myogenic bHLH gene expression; one signal derives from the floor plate/notochord and the other signal derives from regions of the neural tube other than the floor plate. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/7720573/Combinatorial_signals_from_the_neural_tube_floor_plate_and_notochord_induce_myogenic_bHLH_gene_expression_in_the_somite_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=7720573 DB - PRIME DP - Unbound Medicine ER -