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Validity testing of the accuPINCH THC test.
J Anal Toxicol. 1995 Jan-Feb; 19(1):5-12.JA

Abstract

Self-contained drug-testing kits are currently being marketed for a variety of drugs of abuse. These tests are designed to provide rapid access to test results without the need for laboratory facilities. This report describes a validity study of the accuPINCH THC test, a self-contained test for cannabinoids in urine. Three healthy male volunteers with a history of marijuana use participated in the clinical study. Each subject smoked one, two, or four marijuana cigarettes (2.6% THC) on each test day. Urine samples were collected and incorporated into a specimen set consisting of 178 clinical samples, 72 urine samples containing known amounts of drug, and 50 drug-free urine samples. The specimen set was randomized and analyzed under blind conditions by the accuPINCH test and by gas chromatography-mass spectrometry (GC-MS) for 11-nor-9-carboxy-delta 9-tetrahydrocannabinol (THCCOOH). AccuPINCH results were interpreted independently by three readers as positive at either two calibration points (positive A < 100 ng/mL THCCOOH; positive B > or = 100 ng/mL THCCOOH) or negative. Concordance analysis was performed by comparison of the accuPINCH results with GC-MS. In addition, the effects of changes in sample turbidity, temperature, and assay reading time on test outcome were assessed. For the clinical samples, positive B results were associated exclusively with THCCOOH concentrations greater than or equal to 15 ng/mL, whereas positive A and negative results were obtained at all concentrations. All drug-free urine samples were interpreted as either negative or positive A. The test demonstrated relatively low cross-reactivity with THC and other cannabinoids.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Addiction Research Center, NIDA/NIH, Baltimore, MD 21224.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article

Language

eng

PubMed ID

7723303

Citation

Jenkins, A J., et al. "Validity Testing of the accuPINCH THC Test." Journal of Analytical Toxicology, vol. 19, no. 1, 1995, pp. 5-12.
Jenkins AJ, Darwin WD, Huestis MA, et al. Validity testing of the accuPINCH THC test. J Anal Toxicol. 1995;19(1):5-12.
Jenkins, A. J., Darwin, W. D., Huestis, M. A., Cone, E. J., & Mitchell, J. M. (1995). Validity testing of the accuPINCH THC test. Journal of Analytical Toxicology, 19(1), 5-12.
Jenkins AJ, et al. Validity Testing of the accuPINCH THC Test. J Anal Toxicol. 1995 Jan-Feb;19(1):5-12. PubMed PMID: 7723303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validity testing of the accuPINCH THC test. AU - Jenkins,A J, AU - Darwin,W D, AU - Huestis,M A, AU - Cone,E J, AU - Mitchell,J M, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 5 EP - 12 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 19 IS - 1 N2 - Self-contained drug-testing kits are currently being marketed for a variety of drugs of abuse. These tests are designed to provide rapid access to test results without the need for laboratory facilities. This report describes a validity study of the accuPINCH THC test, a self-contained test for cannabinoids in urine. Three healthy male volunteers with a history of marijuana use participated in the clinical study. Each subject smoked one, two, or four marijuana cigarettes (2.6% THC) on each test day. Urine samples were collected and incorporated into a specimen set consisting of 178 clinical samples, 72 urine samples containing known amounts of drug, and 50 drug-free urine samples. The specimen set was randomized and analyzed under blind conditions by the accuPINCH test and by gas chromatography-mass spectrometry (GC-MS) for 11-nor-9-carboxy-delta 9-tetrahydrocannabinol (THCCOOH). AccuPINCH results were interpreted independently by three readers as positive at either two calibration points (positive A < 100 ng/mL THCCOOH; positive B > or = 100 ng/mL THCCOOH) or negative. Concordance analysis was performed by comparison of the accuPINCH results with GC-MS. In addition, the effects of changes in sample turbidity, temperature, and assay reading time on test outcome were assessed. For the clinical samples, positive B results were associated exclusively with THCCOOH concentrations greater than or equal to 15 ng/mL, whereas positive A and negative results were obtained at all concentrations. All drug-free urine samples were interpreted as either negative or positive A. The test demonstrated relatively low cross-reactivity with THC and other cannabinoids.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/7723303/Validity_testing_of_the_accuPINCH_THC_test_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/19.1.5 DB - PRIME DP - Unbound Medicine ER -