Hartland, R P., et al. "The Linkage of (1-3)-beta-glucan to Chitin During Cell Wall Assembly in Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 10, no. 12, 1994, pp. 1591-9.
Hartland RP, Vermeulen CA, Klis FM, et al. The linkage of (1-3)-beta-glucan to chitin during cell wall assembly in Saccharomyces cerevisiae. Yeast. 1994;10(12):1591-9.
Hartland, R. P., Vermeulen, C. A., Klis, F. M., Sietsma, J. H., & Wessels, J. G. (1994). The linkage of (1-3)-beta-glucan to chitin during cell wall assembly in Saccharomyces cerevisiae. Yeast (Chichester, England), 10(12), 1591-9.
Hartland RP, et al. The Linkage of (1-3)-beta-glucan to Chitin During Cell Wall Assembly in Saccharomyces Cerevisiae. Yeast. 1994;10(12):1591-9. PubMed PMID: 7725794.
TY - JOUR
T1 - The linkage of (1-3)-beta-glucan to chitin during cell wall assembly in Saccharomyces cerevisiae.
AU - Hartland,R P,
AU - Vermeulen,C A,
AU - Klis,F M,
AU - Sietsma,J H,
AU - Wessels,J G,
PY - 1994/12/1/pubmed
PY - 1994/12/1/medline
PY - 1994/12/1/entrez
SP - 1591
EP - 9
JF - Yeast (Chichester, England)
JO - Yeast
VL - 10
IS - 12
N2 - Pulse-chase experiments with [14C]glucose demonstrated that in the cell wall of wild-type Saccharomyces cerevisiae alkali-soluble (1-3)-beta-glucan serves as a precursor for alkali-insoluble (1-3)-beta-glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali-insoluble glucan. These cells, unable to form a glucan-chitin complex, were highly vulnerable to hypo-osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall. Conversion of alkali-soluble glucan into alkali-insoluble glucan occurred both early and late during budding and also in the ts-mutant cdc24-1 in the absence of bud formation.
SN - 0749-503X
UR - https://www.unboundmedicine.com/medline/citation/7725794/The_linkage_of__1_3__beta_glucan_to_chitin_during_cell_wall_assembly_in_Saccharomyces_cerevisiae_
L2 - https://doi.org/10.1002/yea.320101208
DB - PRIME
DP - Unbound Medicine