TY - JOUR T1 - The linkage of (1-3)-beta-glucan to chitin during cell wall assembly in Saccharomyces cerevisiae. AU - Hartland,R P, AU - Vermeulen,C A, AU - Klis,F M, AU - Sietsma,J H, AU - Wessels,J G, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 1591 EP - 9 JF - Yeast (Chichester, England) JO - Yeast VL - 10 IS - 12 N2 - Pulse-chase experiments with [14C]glucose demonstrated that in the cell wall of wild-type Saccharomyces cerevisiae alkali-soluble (1-3)-beta-glucan serves as a precursor for alkali-insoluble (1-3)-beta-glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali-insoluble glucan. These cells, unable to form a glucan-chitin complex, were highly vulnerable to hypo-osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall. Conversion of alkali-soluble glucan into alkali-insoluble glucan occurred both early and late during budding and also in the ts-mutant cdc24-1 in the absence of bud formation. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/7725794/The_linkage_of__1_3__beta_glucan_to_chitin_during_cell_wall_assembly_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1002/yea.320101208 DB - PRIME DP - Unbound Medicine ER -