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Structure and polarity of mouse brain synaptic plasma membrane: effects of ethanol in vitro and in vivo.
Biochemistry. 1995 May 02; 34(17):5945-59.B

Abstract

Structural and dielectric alteration by ethanol in vitro and chronic ethanol consumption were examined in synaptic plasma membranes (SPM) using diphenylhexatriene and charged diphenylhexatriene derivatives. These fluorophores, in combination with multifrequency phase and modulation fluorometry, allowed the examination of the surface and interior core of SPM. Limiting anisotropy and rotational relaxation time demonstrated that the synaptosomal plasma membrane surface domain was more rigid than the interior core domain. Ethanol in vitro fluidized the interior core and surface domains in SPM of the control, but not chronic ethanol-treated mice. Although the latter membranes were more rigid than control membranes, the intrinsic rigidity of the interior core of the synaptosomal plasma membrane did not strictly correlate with effects of ethanol in vitro. SPM of irradiated membranes were more rigid, but ethanol fluidized those membranes. Diphenylhexatriene lifetime and photoreactivity were sensitive to the range of dielectric constants in the SPM interior core. Ethanol in vitro increased both the surface and interior core range of dielectric constants of SPM from control but not chronic ethanol-treated animals. Thus, ethanol in vitro altered not only the fluidity but also the range of dielectric constants in both the surface and interior core domains in SPM of control but not chronic ethanol-treated mice.

Authors+Show Affiliations

Veteran's Administration Medical Center, Minneapolis, Minnesota, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7727452

Citation

Colles, S, et al. "Structure and Polarity of Mouse Brain Synaptic Plasma Membrane: Effects of Ethanol in Vitro and in Vivo." Biochemistry, vol. 34, no. 17, 1995, pp. 5945-59.
Colles S, Wood WG, Myers-Payne SC, et al. Structure and polarity of mouse brain synaptic plasma membrane: effects of ethanol in vitro and in vivo. Biochemistry. 1995;34(17):5945-59.
Colles, S., Wood, W. G., Myers-Payne, S. C., Igbavboa, U., Avdulov, N. A., Joseph, J., & Schroeder, F. (1995). Structure and polarity of mouse brain synaptic plasma membrane: effects of ethanol in vitro and in vivo. Biochemistry, 34(17), 5945-59.
Colles S, et al. Structure and Polarity of Mouse Brain Synaptic Plasma Membrane: Effects of Ethanol in Vitro and in Vivo. Biochemistry. 1995 May 2;34(17):5945-59. PubMed PMID: 7727452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and polarity of mouse brain synaptic plasma membrane: effects of ethanol in vitro and in vivo. AU - Colles,S, AU - Wood,W G, AU - Myers-Payne,S C, AU - Igbavboa,U, AU - Avdulov,N A, AU - Joseph,J, AU - Schroeder,F, PY - 1995/5/2/pubmed PY - 1995/5/2/medline PY - 1995/5/2/entrez SP - 5945 EP - 59 JF - Biochemistry JO - Biochemistry VL - 34 IS - 17 N2 - Structural and dielectric alteration by ethanol in vitro and chronic ethanol consumption were examined in synaptic plasma membranes (SPM) using diphenylhexatriene and charged diphenylhexatriene derivatives. These fluorophores, in combination with multifrequency phase and modulation fluorometry, allowed the examination of the surface and interior core of SPM. Limiting anisotropy and rotational relaxation time demonstrated that the synaptosomal plasma membrane surface domain was more rigid than the interior core domain. Ethanol in vitro fluidized the interior core and surface domains in SPM of the control, but not chronic ethanol-treated mice. Although the latter membranes were more rigid than control membranes, the intrinsic rigidity of the interior core of the synaptosomal plasma membrane did not strictly correlate with effects of ethanol in vitro. SPM of irradiated membranes were more rigid, but ethanol fluidized those membranes. Diphenylhexatriene lifetime and photoreactivity were sensitive to the range of dielectric constants in the SPM interior core. Ethanol in vitro increased both the surface and interior core range of dielectric constants of SPM from control but not chronic ethanol-treated animals. Thus, ethanol in vitro altered not only the fluidity but also the range of dielectric constants in both the surface and interior core domains in SPM of control but not chronic ethanol-treated mice. SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/7727452/Structure_and_polarity_of_mouse_brain_synaptic_plasma_membrane:_effects_of_ethanol_in_vitro_and_in_vivo_ DB - PRIME DP - Unbound Medicine ER -