Tags

Type your tag names separated by a space and hit enter

Absence of gluten-specific T lymphocytes in the skin of patients with dermatitis herpetiformis.
J Autoimmun. 1995 Feb; 8(1):75-82.JA

Abstract

Dermatitis Herpetiformis (DH) is an immunobullous skin disease with an associated gluten-sensitive enteropathy. Withdrawal of gluten from the diet leads to the resolution of both the skin lesions and the enteropathy. A T cell-mediated immune response to gluten has been implicated in the damage to the gut; the possible gluten specificity of the T cell infiltrate in DH skin lesions has not, however, been investigated. T cell lines (TCL) were therefore established from the skin lesions of eight patients with DH by culturing skin fragments for 11-17 days with a medium supplemented with 20 U/ml of IL-2. In three cases, gliadin (fraction of gluten toxic to the DH gut) and irradiated, autologous peripheral blood mononuclear cells were also added. The TCL were stained for CD3, CD4, CD8, TCR alpha beta and gamma delta expression by indirect immunofluorescence, and their proliferative responses to mitogens and gluten fraction III (a peptic-tryptic digest of gluten) investigated. Of the eight CD3+ TCL, four were predominantly CD4+ (82.1-98.8%), three predominantly CD8+ (92.6-98.6%) and one TCL contained both 87.6% CD4+ and 95.2% CD8+ T cells, a substantial proportion of which were presumably double-labelled CD4+, CD8+ T cells. All eight TCL, which were almost exclusively TCR alpha beta +, proliferated in response to PHA whilst six out of the eight were stimulated by Concanavalin A. None of the TCL proliferated to gluten fraction III alone; however, two TCL showed increased proliferation to the antigen in the presence of exogenous IL-2 or IL-4 (10 U/ml) compared to cytokine alone.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Dermatology, St Mary's Hospital, Paddington, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7734038

Citation

Baker, B S., et al. "Absence of Gluten-specific T Lymphocytes in the Skin of Patients With Dermatitis Herpetiformis." Journal of Autoimmunity, vol. 8, no. 1, 1995, pp. 75-82.
Baker BS, Garioch JJ, Bokth S, et al. Absence of gluten-specific T lymphocytes in the skin of patients with dermatitis herpetiformis. J Autoimmun. 1995;8(1):75-82.
Baker, B. S., Garioch, J. J., Bokth, S., Leonard, J., & Fry, L. (1995). Absence of gluten-specific T lymphocytes in the skin of patients with dermatitis herpetiformis. Journal of Autoimmunity, 8(1), 75-82.
Baker BS, et al. Absence of Gluten-specific T Lymphocytes in the Skin of Patients With Dermatitis Herpetiformis. J Autoimmun. 1995;8(1):75-82. PubMed PMID: 7734038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of gluten-specific T lymphocytes in the skin of patients with dermatitis herpetiformis. AU - Baker,B S, AU - Garioch,J J, AU - Bokth,S, AU - Leonard,J, AU - Fry,L, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 75 EP - 82 JF - Journal of autoimmunity JO - J Autoimmun VL - 8 IS - 1 N2 - Dermatitis Herpetiformis (DH) is an immunobullous skin disease with an associated gluten-sensitive enteropathy. Withdrawal of gluten from the diet leads to the resolution of both the skin lesions and the enteropathy. A T cell-mediated immune response to gluten has been implicated in the damage to the gut; the possible gluten specificity of the T cell infiltrate in DH skin lesions has not, however, been investigated. T cell lines (TCL) were therefore established from the skin lesions of eight patients with DH by culturing skin fragments for 11-17 days with a medium supplemented with 20 U/ml of IL-2. In three cases, gliadin (fraction of gluten toxic to the DH gut) and irradiated, autologous peripheral blood mononuclear cells were also added. The TCL were stained for CD3, CD4, CD8, TCR alpha beta and gamma delta expression by indirect immunofluorescence, and their proliferative responses to mitogens and gluten fraction III (a peptic-tryptic digest of gluten) investigated. Of the eight CD3+ TCL, four were predominantly CD4+ (82.1-98.8%), three predominantly CD8+ (92.6-98.6%) and one TCL contained both 87.6% CD4+ and 95.2% CD8+ T cells, a substantial proportion of which were presumably double-labelled CD4+, CD8+ T cells. All eight TCL, which were almost exclusively TCR alpha beta +, proliferated in response to PHA whilst six out of the eight were stimulated by Concanavalin A. None of the TCL proliferated to gluten fraction III alone; however, two TCL showed increased proliferation to the antigen in the presence of exogenous IL-2 or IL-4 (10 U/ml) compared to cytokine alone.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0896-8411 UR - https://www.unboundmedicine.com/medline/citation/7734038/Absence_of_gluten_specific_T_lymphocytes_in_the_skin_of_patients_with_dermatitis_herpetiformis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0896-8411(85)70006-6 DB - PRIME DP - Unbound Medicine ER -