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Epstein-Barr virus in B-cell non-Hodgkin's lymphomas: unexpected infection patterns and different infection incidence in low- and high-grade types.
J Pathol. 1995 Mar; 175(3):263-71.JP

Abstract

Two hundred and eight cases of B-cell non-Hodgkin's lymphoma (B-NHL) occurring in Europeans without any signs of HIV infection were investigated for their association with an Epstein-Barr virus (EBV) infection. The polymerase chain reaction (PCR) was applied for EBV-DNA detection, in situ hybridization (ISH) for the cellular localization of EBV-encoded small nuclear RNAs (EBER) and immediate-early RNAs (BHLF), and immunohistology (IH) for the detection of EBV-encoded latent membrane protein (LMP) and EBV nuclear antigen 2 (EBNA2) expression. PCR and EBER-ISH produced congruent results in those cases with amplifiable DNA. EBV was present overall in 26 per cent (54/208) of the B-NHL cases. Through EBER-ISH, the virus could be localized merely in rare non-neoplastic bystander lymphocytes in 27 and additionally in tumour cells of 27 cases. Unexpectedly, the proportion of EBV-infected tumour cells present in the different cases varied between 1 and 100 per cent. All but three of the cases with infected tumour cells were of high-grade malignancy. Correlation with the morphological and immunological tumour phenotype revealed that all cases with more than 80 per cent EBER-positive tumour cells were either B-anaplastic large cell lymphomas (B-ALCL), sporadic Burkitt's lymphomas, or B-NHLs with partial or full plasmacellular differentiation. LMP was consistently absent from Burkitt's lymphomas and constantly expressed in B-ALCLs with EBER-positive tumour cells, while in all other instances it varied greatly and was rarer than EBER expression.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Institute of Pathology, Klinikum Benjamin Franklin, Free University Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7745495

Citation

Hummel, M, et al. "Epstein-Barr Virus in B-cell non-Hodgkin's Lymphomas: Unexpected Infection Patterns and Different Infection Incidence in Low- and High-grade Types." The Journal of Pathology, vol. 175, no. 3, 1995, pp. 263-71.
Hummel M, Anagnostopoulos I, Korbjuhn P, et al. Epstein-Barr virus in B-cell non-Hodgkin's lymphomas: unexpected infection patterns and different infection incidence in low- and high-grade types. J Pathol. 1995;175(3):263-71.
Hummel, M., Anagnostopoulos, I., Korbjuhn, P., & Stein, H. (1995). Epstein-Barr virus in B-cell non-Hodgkin's lymphomas: unexpected infection patterns and different infection incidence in low- and high-grade types. The Journal of Pathology, 175(3), 263-71.
Hummel M, et al. Epstein-Barr Virus in B-cell non-Hodgkin's Lymphomas: Unexpected Infection Patterns and Different Infection Incidence in Low- and High-grade Types. J Pathol. 1995;175(3):263-71. PubMed PMID: 7745495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus in B-cell non-Hodgkin's lymphomas: unexpected infection patterns and different infection incidence in low- and high-grade types. AU - Hummel,M, AU - Anagnostopoulos,I, AU - Korbjuhn,P, AU - Stein,H, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 263 EP - 71 JF - The Journal of pathology JO - J. Pathol. VL - 175 IS - 3 N2 - Two hundred and eight cases of B-cell non-Hodgkin's lymphoma (B-NHL) occurring in Europeans without any signs of HIV infection were investigated for their association with an Epstein-Barr virus (EBV) infection. The polymerase chain reaction (PCR) was applied for EBV-DNA detection, in situ hybridization (ISH) for the cellular localization of EBV-encoded small nuclear RNAs (EBER) and immediate-early RNAs (BHLF), and immunohistology (IH) for the detection of EBV-encoded latent membrane protein (LMP) and EBV nuclear antigen 2 (EBNA2) expression. PCR and EBER-ISH produced congruent results in those cases with amplifiable DNA. EBV was present overall in 26 per cent (54/208) of the B-NHL cases. Through EBER-ISH, the virus could be localized merely in rare non-neoplastic bystander lymphocytes in 27 and additionally in tumour cells of 27 cases. Unexpectedly, the proportion of EBV-infected tumour cells present in the different cases varied between 1 and 100 per cent. All but three of the cases with infected tumour cells were of high-grade malignancy. Correlation with the morphological and immunological tumour phenotype revealed that all cases with more than 80 per cent EBER-positive tumour cells were either B-anaplastic large cell lymphomas (B-ALCL), sporadic Burkitt's lymphomas, or B-NHLs with partial or full plasmacellular differentiation. LMP was consistently absent from Burkitt's lymphomas and constantly expressed in B-ALCLs with EBER-positive tumour cells, while in all other instances it varied greatly and was rarer than EBER expression.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/7745495/Epstein_Barr_virus_in_B_cell_non_Hodgkin's_lymphomas:_unexpected_infection_patterns_and_different_infection_incidence_in_low__and_high_grade_types_ L2 - https://doi.org/10.1002/path.1711750303 DB - PRIME DP - Unbound Medicine ER -